Methods of producing recombinant heme-binding proteins and uses thereof

ABSTRACT

The present invention is directed to methods of producing recombinant functional heme-binding proteins with complete heme incorporation and purified preparations of the same. The present invention is further directed to methods of identifying agents that modulate the activity of heme-binding proteins.

This application claims the benefit of U.S. Provisional Patent Application Ser. No. 61/347,193, filed May 21, 2010, which is hereby incorporated by reference in its entirety.

This invention was made with government support under grant number NCHE-0749997 awarded by the National Science Foundation and grant number R01GM079679 awarded by the National Institutes of Health. The government has certain rights in this invention.

FIELD OF THE INVENTION

The present invention is directed to methods of producing recombinant heme-binding proteins with complete heme incorporation and methods of using the same.

BACKGROUND OF THE INVENTION

Heme proteins encompass a wide range of functions that include electron transfer, transport and storage of oxygen, production and sensing of nitric oxide, decomposition of reactive oxygen species, catalytic oxidation of substrates, signal transduction and control of gene expression (Gray et al., “Electron Transfer in Engineered Heme Enzymes,” Faseb. J. 11:A781-A781 (1997); Sono et al., “Heme-Containing Oxygenases,” Chem. Rev. 96:2841-2888 (1996); Alderton et al., “Nitric Oxide Synthases Structure, Function and Inhibition,” Biochem. J. 357:593-615 (2001); and Perutz et al., “A Haemoglobin that Acts as an Oxygen Sensor: Signalling Mechanism and Structural Basis of its Homology with PAS Domains,” Chem. Biol. 6:R291-R297 (1999)). Under incorporation of heme into recombinant proteins can adversely influence their characterization. Evaluation of enzymatic activity is systematically underestimated if a significant proportion of the recombinant sample does not contain heme. Furthermore, proteins devoid of heme may bind another non-native cofactor with a contaminating activity of its own. A pure protein sample is usually essential for structural characterization by techniques such as X-ray crystallography, as this method often requires a single, well-folded species for crystallization. Other methods of heme protein characterization are less sensitive to the level of heme incorporation, particularly if those methods directly detect the metallocofactor (e.g., UV/Vis, EPR, and Mossbauer spectroscopy) or rely on enzymatic activity. This is both good and bad in that under incorporation may not greatly affect the analysis, but heterogeneity in the sample may go undetected.

Incomplete heme incorporation into recombinant proteins has been a frequently encountered problem (Varadarajan et al., “Cloning, Expression in Escherichia-Coli, and Reconstitution of Human Myoglobin,” Pro. Nat. Acad. Sci. U.S.A. 82:5681-5684 (1985); Ishikawa et al., “Expression of Rat Heme Oxygenase in Escherichia-Coli as a Catalytically Active, Full-Length Form that Binds to Bacterial-Membranes,” Eur. J. Biochem. 202:161-165 (1991); Smith et al., “Expression of a Synthetic Gene for Horseradish Peroxidase-C in Escherichia-Coli and Folding and Activation of the Recombinant Enzyme with Ca-2+ and Heme,” J. Biol. Chem. 265:13335-13343 (1990); Kery et al., “Delta-Aminolevulinate Increases Heme Saturation and Yield of Human Cystathionine Beta-Synthase Expressed in Escherichia-Coli,” Archives Biochem. Biophys. 31:624-29 (1995); Varnado et al., “Properties of a Novel Periplasmic Catalase-Peroxidase from Escherichia Coli O157: H7,” Archives Biochem. Biophys. 42:1166-174 (2004); and Graves et al., “Enhancing Stability and Expression of Recombinant Human Hemoglobin in E-Coli: Progress in the Development of a Recombinant HBOC Source,” Biochimica Et Biophysica Acta-Proteins And Proteomics 1784:1471-1479 (2008)) and thus, techniques have been developed to improve heme loading (Kery et al., “Delta-Aminolevulinate Increases Heme Saturation and Yield of Human Cystathionine Beta-Synthase Expressed in Escherichia-Coli,” Archives Biochem. Biophys. 31:624′29 (1995); Varnado et al., “Properties of a Novel Periplasmic Catalase-Peroxidase from Escherichia Coli O157: H7,” Archives Biochem. Biophys. 42:1166-174 (2004); Graves et al., “Enhancing Stability and Expression of Recombinant Human Hemoglobin in E-Coli: Progress in the Development of a Recombinant HBOC Source,” Biochimica Et Biophysica Acta-Proteins And Proteomics 1784:1471-1479 (2008); Weickert et al., “Optimization of Heterologous Protein Production in Escherichia Coli,” Curr. Opinion In Biotechnol. 7:494-499 (1996); Shen et al., “Production of Unmodified Human Adult Hemoglobin in Escherichia-Coli,” Pro. Nat, Acad. Sci. U.S.A. 90:8108-8112 (1993); Varnado et al., “System for the Expression of Recombinant Hemoproteins in Escherichia Coli,” Prot. Exp. Pur. 35:76-83 (2004); and Vaniado et al., “Expression of Recombinant Hemoproteins in E. Coli Using a Heme Protein Expression System,” Biophys. J. 384A-384A (2007)). During induction of recombinant protein expression from highly active vectors, such as those that employ the T7-polymerase, a population of protein will fold without the heme co-factor under conditions where folding outpaces heme delivery (Weickert et al., “Blackmore, Stabilization of Apoglobin by Low Temperature Increases Yield of Soluble Recombinant Hemoglobin in Escherichia Coli,” App. Environ. Microbiol. 63:4313-4320 (1997)). Supplementing the growth media with δ-amino levulinic acid (d-ALA), a precursor in the C5 heme biosynthesis pathway, increases levels of heme biosynthesis and thereby heme incorporation into the target protein (Kery et al., “Delta-Aminolevulinate Increases Heme Saturation and Yield of Human Cystathionine Beta-Synthase Expressed in Escherichia-Coli,” Archives Biochem. Biophys. 31:624-29 (1995); Pcsce ct al., “The 109 Residue Nerve Tissue Minihemoglobin from Cerebratulus Lacteus Highlights Striking Structural Plasticity of the Alpha-Helical Globin Fold,” Structure 10:725-735 (2002); and Summerford et al., “Bacterial Expression of Scapharca Dimeric Hemoglobin—A Simple-Model System for Investigating Protein Cooperativity,” Prot. Engineer. 8:593-599 (1995)). Increased heme biosynthesis rates through d-ALA supplementation does not achieve complete heme incorporation into all heme-binding proteins (Weickert et al., “Optimization of Heterologous Protein Production in Escherichia Coli,” Curr. Opinion In Biotechnol. 7:494-499 (1996); Shen et al., “Production of Unmodified Human Adult Hemoglobin in Escherichia-Coli,” Pro. Nat. Acad. Sci. U.S.A. 90:8108-8112 (1993); Weickert et al., “High-Fidelity Translation of Recombinant Human Hemoglobin in Escherichia Coli,” Appl. Environ. Microbiol. 64:589-1593 (1998); and Wcickert et al., “A Mutation that Improves Soluble Recombinant Hemoglobin Accumulation in Escherichia Coli in Heme Excess,” App. Environ. Microbiol. 65:640-647 (1999)).

Another technique for increasing home incorporation into recombinant proteins involves supplying the bacteria with hemin in the growth media. However, most E. coli strains do not possess an efficient heme transport system, and thus uptake of hemin relies on diffusion through the cell membrane. As a result, hemin feeding is much more effective with strains that co-express heme transport genes from other gram-negative bacteria, along with the heme-protein of interest (Graves et al., “Enhancing Stability and Expression of Recombinant Human Hemoglobin in E-Coli: Progress in the Development of a Recombinant HBOC Source,” Biochimica Et Biophysica Acta—Proteins And Proteomics 1784:1471-1479 (2008); Varnado et al., “System for the Expression of Recombinant Hemoproteins in Escherichia Coli,” Prot. Exp. Pur. 35:76-83 (2004); and Varnado et al., “Expression of Recombinant Hemoproteins in E. Coli Using a Heme Protein Expression System,” Biophys. J. 384A-384A (2007)). For example, co-expression of the heme transport system from P. shigelloides, which consists of the proteins Hug A/B/C/D, TonB, and Exb B/D, while also supplementing the growth media with hemin, results in higher amounts of the target holo-protein (in this case hemoglobin) (Graves et al., “Enhancing Stability and Expression of Recombinant Human Hemoglobin in E-Coli: Progress in the Development of a Recombinant HBOC Source,” Biochimica Et Biophysica Acta—Proteins And Proteomics 1784:1471-1479 (2008)). A similar method involves the co-expression of the heme receptor ChuA from E. coli. strain O157:H7 to enhance hemin (Varnado et al., “System for the Expression of Recombinant Hemoproteins in Escherichia Coli,” Prot. Exp. Pur. 35:76-83 (2004)). This latter method also shows a significant increase in the amount of heme-loaded protein generated, although in both cases, the ratio of holoprotein:apoprotein was not evaluated. Another approach utilizes the heme-permeability of E. coli strain RP523, which has the hem B, porphobilinogen synthase gene disrupted to prevent native heme synthesis. All heme and/or heme analogs are procured by the cells from the growth media and incorporation is nearly stoichiometric for proteins expressed in the cytoplasm (0.8-1.0 heme/heme analog per protein) (Woodward et al., “An Escherichia Coli Expression-Based Method for Heme Substitution,” Nat. Methods 4:43-45 (2007)).

Full incorporation of heme in recombinant proteins is also important for commercial applications. For example, the feasibility of employing recombinant human hemoglobin as an oxygen delivery pharmaceutical is limited by the yield of holoprotein that can be made in E. coli (Graves et al. “Enhancing Stability and Expression of Recombinant Human Hemoglobin in E-Coli: Progress in the Development of a Recombinant HBOC Source,” Biochimica Et Biophysica Acta—Proteins And Proteomics 1784:1471-1479 (2008)). Some of the methods discussed above, while effective, require co-expression of several heme transport proteins, which could limit yields, and/or require addition of the heme cofactor.

The present invention is directed to overcoming these and other deficiencies in the art.

SUMMARY OF THE INVENTION

A first aspect of the present invention relates to a method of producing a functional recombinant heme-binding protein. This method involves co-expressing a recombinant heme-binding protein and recombinant ferrochelatase protein, or polypeptide thereof, under conditions effective for complete heme incorporation into the recombinantly produced heme-binding protein, thereby producing a functional heme-binding protein.

A second aspect of the present invention relates to a system for producing functional heme-binding proteins. This system comprises an expression system and one or more expression constructs encoding a recombinant heme-binding protein and a recombinant ferrochelatase.

A third aspect of the present invention relates to a purified preparation of recombinant functional heme-binding protein.

A fourth aspect of the present invention relates to a method of identifying an agent that modulates activity of a heme-binding protein. This method involves providing a candidate agent and providing a recombinant functional heme-binding protein. This method further involves contacting the candidate agent with the recombinant functional heme-binding protein under conditions at which the functional heme-binding protein is active and comparing the activity of the functional heme-binding protein as a result of said contacting to the activity of the heme-binding protein alone, both under said conditions at which the heme-binding protein is active. A candidate agent that modulates the activity of a heme-binding protein is identified based on said comparing.

Another aspect of the present invention relates to a method of evaluating the metabolism of an agent by a heme-binding protein. This method involves providing a candidate agent and providing a recombinant functional heme-binding protein. This method further involves contacting the candidate agent with the recombinant functional home-binding protein under conditions at which the functional heme-binding protein is active and comparing the activity of the functional heme-binding protein as a result of said contacting to the activity of the heme-binding protein alone, both under said conditions at which the heme-binding protein is active. The metabolism of the candidate agent by a heme-binding protein is evaluated based on said comparing.

The present invention is a straightforward and inexpensive method for high fidelity incorporation of heme into recombinantly overexpressed heme proteins. Co-expression of just one native protein, ferrochelatase (FC), in the presence of δ-ALA is sufficient to achieve 100% heme incorporation into three unrelated home proteins derived from different organisms. Since pre-existing methods of recombinant heme-binding protein production result in sub-optimal heme incorporation and varying amounts of protein production depending on the protein of interest, the ability to achieve complete heme incorporation as described herein, has important implications for heme-binding protein biochemical characterization, spectroscopy, structural studies, and for the production of homogeneous commercial heme-binding proteins with high activity.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the UV-Vis spectra of Geobacillus stearothermophilus nitric oxide synthase (gsNOS) expressed alone (thin line) and gsNOS expressed with ferrochelatase (FC) (thick line). Co-expression of FC results in a substantial increase in home content of gsNOS as measured by the Abs₄₀₃/Abs₂₈₀ ratio. This value saturates at 0.6, which indicates nearly complete heme incorporation. The inset of FIG. 1 is an immunoblot showing that GsNOS expressed by itself results in two bands on SDS-PAGE (lane A), both of which shift on His-tag cleavage (lane B), GsNOS co-expressed with FC results in just one band (lane C), that shifts on His-tag cleavage (lane D) as expected.

FIGS. 2A-2B are Resonance Raman and fluorescence spectra of recombinantly expressed gsNOS. The Resonance Raman spectra from gsNOS co-expressed with FC is shown as FIG. 2A(i), and gsNOS expressed by itself is depicted in FIGS. 2A(ii) and 2A(iii). The spectrum in FIG. 2A(ii) is obtained with 3 mW of 413.1 nm laser excitation with an acquisition time of 5 min. The spectrum in FIG. 2A(iii) is from the same sample as FIG. 2A(ii), but obtained after prolonged irradiation with 42 mW of 413.1 nm laser for two hours. FIG. 2A(iv) shows the difference spectrum (i.e., the spectrum of FIG. 2A(ii)—the spectrum of FIG. 2A(iii)), which highlights spectral lines resulting from contamination by photosensitive protoporphyrin IX, The fluorescence spectrum shown in FIG. 2B obtained after a 397 nm excitation is representative of that of protoporphyrin IX bound to protein (see infra), and is from the same sample as FIG. 2B.

FIG. 3 shows the UV-Vis spectra of BP450 expressed by itself (thin line) and BP450 expressed with FC (thick line). Co-expression of FC results in a substantial increase in heme content of BP450 (Abs_(Soret)/Abs₂₈₀). The inset of FIG. 3 is an immunoblot showing that BP450 expressed alone also results in two bands (lane A), both of which shift on His-tag cleavage (lane B). BP450 co-expressed with FC results in one band (lane C), which shifts on His-tag cleavage as expected (lane D).

FIG. 4 shows the UV-Vis spectra of a Heme Binding PAS domain (HBPAS; His-ligated). When expressed by itself (thin line), the UV-Vis spectra of HBPAS shows four Q-hand absorption peaks (FIG. 4 inset). Co-expression with FC (thick line) increases the heme content and results in only two Q-bands (inset).

FIG. 5 shows the UV-Vis spectra of full-length NOS containing a reductase domain (>100 KdA). Under normal conditions of expression the Soret band indicative of heme incorporation is barely visible above the flavin absorption of the reductase component (gray trace), however, upon co-expression with ferrochelatase a strong Soret absorption peak (at ˜416 nm), indicative of heme incorporation becomes apparent (black trace), Total yields of full-length NOS also increase in the presence of ferrochelatase.

DETAILED DESCRIPTION OF THE INVENTION

A first aspect of the present invention relates to a method of producing functional recombinant heme-binding proteins. This method involves co-expressing a recombinant heme-binding protein and recombinant ferrochelatase protein, or polypeptide thereof, under conditions effective for complete heme incorporation into the recombinantly produced heme-binding protein, thereby producing a functional heme-binding protein.

In accordance with this aspect of the invention, a heme-binding protein encompasses any protein that contains a heme prosthetic group either covalently or noncovalently bound to itself. Heme-binding proteins have diverse biological functions including, oxygen transport, catalysis, active membrane transport, electron transport, and sensory. The various classes of heme-binding proteins that are encompassed by the methods of the present invention include, without limitation, globins (e.g., hemoglobin, myoglobin, neuroglobin, cytoglobin, leghemoglobin), cytochromes (e.g., a-, b-, and c-types, cd1-nitrite reductase, cytochrome oxidase), transferrins (e.g., lactotransferrin, serotransferrin, melanotransferrin), bacterioferririns, hydroxylamine oxidoreductase, nitrophorins, peroxidases (e.g., lignin peroxidase), cyclooxygenases (e.g., COX-1, COX-2, COX-3, prostaglandin H synthase), catalases, cytochrome P-450s, chloroperoxidases, PAS-domain heme sensors, H-NOX heme sensors (e.g., soluble guanylate cyclase, FixL, DOS, HemAT, and CooA), heme-oxygenases, and nitric oxide synthases. The recombinant heme-binding protein produced using the methods of the present invention can be prokaryotic or eukaryotic. For example, in one embodiment of the present invention the recombinant heme-binding protein is mammalian, preferably human. In another embodiment of the present invention the recombinant heme-binding protein is bacterial. In yet another embodiment of the present invention, the recombinant heme-binding protein is fungal, preferably yeast.

Table 1 provides a non-exhaustive list of exemplary human heme-binding proteins that are suitable for production using the methods of the present invention. Table 1 identifies each heme-binding protein by its Universal Protein Resource Knowledgebase (UniProtKB)/Swiss Prot accession number, which provides the amino acid sequence of the identified protein, and the EMBL Nucleotide Database accession number, which provides the nucleotide sequence encoding the heme-binding protein. The UniProtKB/Swiss Prot and EMBL accession numbers, along with the corresponding amino acid and nucleotide sequence information for each entry in Table 1 is hereby incorporated by reference. Table 1 further identifies the UniProtKB/Swiss Prot entry name, protein names and gene names for each identified heme-binding protein.

Table 2 provides a list of lignin peroxidases, which are heme-binding proteins also suitable for production using the methods of the present invention. As described herein, lignin peroxidases metabolism the lignin of plant cell walls which facilitates the breakdown of cell wall polysaccharides to simple sugars and the subsequent conversion of these sugars to usable bio-fuel. Table 2 identifies each lignin peroxidase by its UniProtKB/Swiss Prot accession number, which provides the amino acid sequence of the protein, and the EMBL Nucleotide Database accession number, which provides the encoding nucleotide sequence. The UniProtKB/Swiss Prot and EMBL accession numbers, along with the corresponding amino acid and nucleotide sequence information for each entry in Table 2 is hereby incorporated by reference. Table 2 further identifies the UniProtKB/Swiss Prot entry name, protein names, protein family, gene names, and organism for each entry.

Traditional methods of producing recombinant heme-binding proteins generate recombinant proteins having incomplete heme incorporation. Under incorporation of heme into recombinant proteins can adversely influence their function. In contrast to these traditional methods, the methods of the present invention generate recombinant heme-binding proteins that have complete heme incorporation. Therefore, these proteins are completely functional and are more suitable for research, clinical, and commercial applications. Incomplete heme incorporation into a recombinant protein can be detected by the presence of free base porphyrin using fluorescence spectroscopy

TABLE 1 Human Heme-Binding Proteins Heme- UniProtKB/ binding Swiss-Prot Protein Accession Class No. Entry name Protein names Gene names EMBL Accession No. Globins Q8WWM9 CYGB_HUMAN Cytoglobin (Histoglobin) CYGB STAP AJ315162; AB057769; AK098057; (HGb) (Stellate cell CH471099; BC029798; activation-associated protein) P09105 HBAT_HUMAN Hemoglobin subunit theta-1 HBQ1 X06482; M33022; DQ431198; AE006462; (Hemoglobin theta-1 BC056686; M91453; chain) (Theta-1-globin) P02008 HBAZ_HUMAN Hemoglobin subunit zeta HBZ HBZ2 J00182; M24173; Z84721; CR456848; (HBAZ) (Hemoglobin zeta AE006462; CH471112; BC027892; chain) (Zeta-globin) P69905 HBA_HUMAN Hemoglobin subunit alpha HBA1; HBA2 J00153; J00153; V00491; V00493; (Alpha-globin) (Hemoglobin V00488; V00516; AF230076; AF525460; alpha chain) DQ431198; DQ431198; AF097635; AF105974; AF349571; AF536204; DQ499017; DQ499018; AK223392; AE006462; AE006462; Z84721; Z84721; BC005931; BC008572; BC032122; BC050661; BC101846; BC101848; P68871 HBB_HUMAN Hemoglobin subunit beta HBB M25079; V00499; DQ126270; (Beta-globin) (Hemoglobin DQ126271; DQ126272; DQ126273; beta chain) [Cleaved into: DQ126274; DQ126275; DQ126276; LVV-hemorphin-7] DQ126277; DQ126278; DQ126279; DQ126280; DQ126281; DQ126282; DQ126283; DQ126284; DQ126285; DQ126286; DQ126287; DQ126288; DQ126289; DQ126290; DQ126291; DQ126292; DQ126293; DQ126294; DQ126295; DQ126296; DQ126297; DQ126298; DQ126299; DQ126300; DQ126301; DQ126302; DQ126303; DQ126304; DQ126305; DQ126306; DQ126307; DQ126308; DQ126309; DQ126310; DQ126311; DQ126312; DQ126313; DQ126314; DQ126315; DQ126316; DQ126317; DQ126318; DQ126319; DQ126320; DQ126321; DQ126322; DQ126323; DQ126324; DQ126325; AF007546; AF083883; AF117710; AF181989; AF349114; AF527577; AY136510; AY163866; AY260740; AY509193; EF450778; EU694432; AK311825; CR536530; CR541913; CH471064; BC007075; U01317; V00497; V00500; L26462; L26463; L26464; L26465; L26466; L26467; L26468; L26469; L26470; L26471; L26472; L26473; L26 P02042 HBD_HUMAN Hemoglobin subunit delta HBD U01317; V00505; AF339401; AF339402; (Delta-globin) (Hemoglobin AF339403; AF339404; AF339405; delta chain) AF339406; AF339407; AF339408; AF339409; AF339410; AF339411; AF339412; AF339413; AF339414; AF339415; AF339416; AF339417; AY034468; DQ157442; BC069307; BC070282; P02100 HBE_HUMAN Hemoglobin subunit HBE1 HBE U01317; V00508; CR541912; epsilon (Epsilon-globin) CH471064; BC015537; (Hemoglobin epsilon chain) P69891 HBG1_HUMAN Hemoglobin subunit gamma- HBG1 PRO2979 M91036; M91037; V00513; V00514; 1 (Gamma-1-globin) (Hb J00176; U01317; AF130098; F Agamma) (Hemoglobin CH471064; BC010913; BC020719; gamma-1 chain) (Hemoglobin AF487523; gamma-A chain) P69892 HBG2_HUMAN Hemoglobin subunit gamma- HBG2 M91036; M91037; U01317; V00515; 2 (Gamma-2-globin) (Hb M15386; AY662983; AK290492; F Ggamma) (Hemoglobin BC010914; BC029387; BC130457; gamma-2 chain) BC130459; M11427; (Hemoglobin gamma-G chain) Q6B0K9 HBM_HUMAN Hemoglobin subunit mu HBM HBAP2 AY698022; DQ431198; BC035682; (Hemoglobin mu chain) (Mu-globin) P02144 MYG_HUMAN Myoglobin MB X00371; X00372; X00373; M14603; M10090; M14602; CR456516; CR541949; DQ003030; AL022334; AL049747; BC014547; Q9NPG2 NGB_HUMAN Neuroglobin NGB AJ245944; AJ245946; AF422796; AF422797; AC007375; AC007954; BC032509; Cyto- P15538 C11B1_HUMAN Cytochrome P450 11B1, CYP11B1 S11BH M32879; M32863; M32878; X55764; chrome mitochondrial (CYPXIB1) D16153; D16155; EU332839; P450s (Cytochrome P-450c11) CH471162; BC096287; M24667; D10169; (Cytochrome P450C11) (Steroid 11-beta- hydroxylase) (EC 1.14.15.4) P19099 C11B2_HUMAN Cytochrome P450 11B2, CYP11B2 M32881; M32864; M32880; X54741; mitochondrial D13752; EU326306; CH471162; (Aldosterone synthase) (ALDOS) (EC 1.14.15.4) (EC 1.14.15.5) (Aldosterone-synthesizing enzyme) (CYPXIB2) (Cytochrome P-450Aldo) (Cytochrome P-450C18) (Steroid 18-hydroxylase) A6NCC7 C21AL_HUMAN Putative cytochrome P450 21-like protein ENSP00000364438 Q4G0S4 C27C1_HUMAN Cytochrome P450 27C1 CYP27C1 AK131190; BC039307; (EC 1.14.—.—) Q6ZSU1 C2G1L_HUMAN Putative cytochrome P450 AK127151; 2G1-like protein P05108 CP11A_HUMAN Cholesterol side-chain CYP11A1 CYP11A M14565; X05367; X05368; X05369; cleavage enzyme, X05370; X05371; X05372; X05373; mitochondrial (EC X05374; AK292300; CH471136; 1.14.15.6) (CYPXIA1) BC032329; X14257; M28253; (Cholesterol desmolase) (Cytochrome P450 11A1) (Cytochrome P450(scc)) P05093 CP17A_HUMAN Steroid 17-alpha- CYP17A1 CYP17 M14564; M19489; M63871; M31153; hydroxylase/17,20 lyase S17AH M31146; M31147; M31148; M31149; (EC 1.14.99.9) (CYPXVII) M31150; M31151; M31152; BT020000; (Cytochrome P450 17A1) AL358790; BC062997; BC063388; (Cytochrome P450-C17) (Cytochrome P450c17) (Steroid 17-alpha- monooxygenase) P11511 CP19A_HUMAN Cytochrome P450 19A1 (EC CYP19A1 ARO1 M22246; X13589; M18856; J04127; 1.14.14.1) (Aromatase) CYAR CYP19 Y07508; M30804; M30796; M30797; (CYPXIX) (Cytochrome P- M30798; M30800; M30801; M30802; 450AROM) (Estrogen M30803; AY957953; BC107785; synthase) M28420; P04798 CP1A1_HUMAN Cytochrome P450 1A1 (EC CYP1A1 X02612; K03191; X04300; AF253322; 1.14.14.1) (CYPIA1) AK223113; BC023019; M12079; (Cytochrome P450 form 6) AF040259; (Cytochrome P450- C) (Cytochrome P450-P1) P05177 CP1A2_HUMAN Cytochrome P450 1A2 (EC CYP1A2 Z00036; L00389; L00384; L00385; 1.14.14.1) (CYPIA2) L00386; L00388; L00387; M31667; (Cytochrome P(3)450) M31664; M31665; M31666; M12078; (Cytochrome P450 4) AF182274; AF253322; DQ022432; (Cytochrome P450-P3) BC067424; BC067425; BC067426; BC067427; BC067428; M55053; Q16678 CP1B1_HUMAN Cytochrome P450 1B1 (EC CYP1B1 U03688; U56438; AF450132; 1.14.14.1) (CYPIB1) AF450131; BT019979; AY393998; BC012049; AF171066; Q6UW02 CP20A_HUMAN Cytochrome P450 20A1 (EC CYP20A1 AY359068; AC011737; BC020616; 1.14.—.—) UNQ667/PRO1301 BC033752; P08686 CP21A_HUMAN Steroid 21-hydroxylase (EC CYP21A2 CYP21 M12792; M13936; M26856; X58906; 1.14.99.10) (21-OHase) CYP21B BC125182; K02771; M19711; M17252; (Cytochrome P-450c21) (Cytochrome P450 21) (Cytochrome P450 XXI) (Cytochrome P450-C21) (Cytochrome P450-C21B) Q07973 CP24A_HUMAN 1,25-dihydroxyvitamin CYP24A1 CYP24 L13286; AL138805; U60669; S67623; D(3) 24-hydroxylase, mitochondrial (24-OHase) (Vitamin D(3) 24- hydroxylase) (EC 1.14.13.n4) (Cytochrome P450 24A1) (Cytochrome P450-CC24) O43174 CP26A_HUMAN Cytochrome P450 26A1 (EC CYP26A1 CYP26 AF005418; AL358613; CH471066; 1.14.—.—) (Cytochrome P450RAI1 P450 retinoic acid- inactivating 1) (Cytochrome P450RAI) (hP450RAI) (Retinoic acid 4-hydroxylase) (Retinoic acid-metabolizing cytochrome) Q9NR63 CP26B_HUMAN Cytochrome P450 26B1 (EC CYP26B1 CYP26A2 AF252297; AC007002; AK313433; 1.14.—.—) (Cytochrome P450 P450RAI2 BC069443; BC109205; 26A2) (Cytochrome P450 retinoic acid-inactivating 2) (Cytochrome P450RAI-2) (Retinoic acid-metabolizing cytochrome) Q8V0L0 CP26C_HUMAN Cytochrome P450 26C1 (EC CYP26C1 AY356349; AL358613; 1.14.—.—) Q02318 CP27A_HUMAN Sterol 26-hydroxylase, CYP27A1 CYP27 M62401; X59812; AY178622; mitochondrial (EC AK290418; CH471063; BC040430; 1.14.13.15) BC051851; S62709; (5-beta-cholestane-3- alpha,7-alpha,12-alpha- triol 27-hydroxylase) (Cytochrome P-450C27/25) (Cytochrome P450 27) (Sterol 27-hydroxylase) (Vitamin D(3) 25-hydroxylase) O15528 CP27B_HUMAN 25-hydroxyvitamin D-1 CYP27B1 AF027152; AB005038; AB005989; alpha hydroxylase, CYP1ALPHA AB005990; AB006987; AF020192; mitochondrial (EC CYP27B AF246895; AY288916; 1.14.13.13) (25-OHD-1 alpha-hydroxylase) (25- hydroxyvitamin D(3) 1- alpha-hydroxylase) (VD3 1A hydroxylase) (Calcidiol 1- monooxygenase) (Cytochrome P450 subfamily XXVIIB polypeptide 1) (Cytochrome P450C1 alpha) (Cytochrome P450VD1-alpha) (Cytochrome p450 27B1) P11509 CP2A6_HUMAN Cytochrome P450 2A6 (EC CYP2A6 CYP2A3 X13897; X13929; X13930; M33318; 1.14.14.1) (CYPIIA6) AF182275; AK312964; EU135979; (Coumarin 7-hydroxylase) FJ440681; CH471126; BC096253; (Cytochrome P450 IIA3) BC096254; BC096255; BC096256; (Cytochrome P45D(I)) AF326721; K03192; P20853 CP2A7_HUMAN Cytochrome P450 2A7 (EC CYP2A7 M33317; U22029; 1.14.14.1) (CYPIIA7) (Cytochrome P450 IIA4) Q16696 CP2AD_HUMAN Cytochrome P450 2A13 (EC CYP2A13 U22028; AF209774; AY513604; 1.14.14.1) (CYPIIA13) AY513605; AY513606; AY513608; AY513609; P20813 CP2B6_HUMAN Cytochrome P450 2B6 (EC CYP2B6 M29874; AF182277; DQ298753; 1.14.14.1) (CYPIIB6) AC023172; (Cytochrome P450 IIB1) P10632 CP2C8_HUMAN Cytochrome P450 2C8 (EC CYP2C8 M17397; M17398; Y00498; AK292753; 1.14.14.1) (CYPIIC8) AK315823; AY514490; AL359672; (Cytochrome P450 IIC2) CH471066; BC020596; X54807; (Cytochrome P450 MP-12) M21941; M21942; X51535; (Cytochrome P450 MP-20) (Cytochrome P450 form 1) (S-mephenytoin 4- hydroxylase) P11712 CP2C9_HUMAN Cytochrome P450 2C9 ((R)- CYP2C9 CYP2C10 AY341248; AY702706; D00173; limonene 6-monooxygenase) M15331; M21939; M21940; (EC 1.14.13.80) ((S)- S46963; limonene 6-monooxygenase) (EC 1.14.13.48) ((S)- limonene 7-monooxygenase) (EC 1.14.13.49) (CYPIIC9) (Cytochrome P-450MP) (Cytochrome P450 MP-4) (Cytochrome P450 MP-8) (Cytochrome P450 PB-1) (S- mephenytoin 4-hydroxylase) P33260 CP2CI_HUMAN Cytochrome P450 2C18 (EC CYP2C18 M61856; L16876; L16871; L16872; 1.14.14.1) (CYPIIC18) L16869; L16870; L16875; L16873; (Cytochrome P450-6b/29c) L16874; AK313403; AL583836; CH471066; BC069666; BC096257; BC096258; P33261 CP2CJ_HUMAN Cytochrome P450 2C19 ((R)- CYP2C19 M61854; M61858; L07093; AY796203; limonene 6-monooxygenase) AL583836; AL133513; AL133513; (EC 1.14.13.80) ((S)- AL583836; L39098; L39097; L39102; limonene 6-monooxygenase) L39099; L39100; L39101; (EC 1.14.13.48) ((S)- limonene 7-monooxygenase) (EC 1.14.13.49) (CYPIIC17) (CYPIIC19) (Cytochrome P450-11A) (Cytochrome P450-254C) (Mephenytoin 4- hydroxylase) P10635 CP2D6_HUMAN Cytochrome P450 2D6 (EC CYP2D6 CYP2DL1 M20403; X08006; M33388; AY545216; 1.14.14.1) (CYPIID6) DQ282144; DQ282145; DQ282146; (Cytochrome P450-DB1) DQ282151; DQ282154; DQ282155; (Debrisoquine 4- BC075023; BC075024; hydroxylase) P05181 CP2E1_HUMAN Cytochrome P450 2E1 (EC CYP2E1 CYP2E J02625; J02843; AF182276; DQ515958; 1.14.13.—) (4-nitrophenol AL161645; CH471211; AF084225; 2-hydroxylase) (EC D50111; 1.14.13.n7) (CYPIIE1) (Cytochrome P450-J) P24903 CP2F1_HUMAN Cytochrome P450 2F1 (EC CYP2F1 J02906; AF372573; AF372570; 1.14.14.1) (CYPIIF1) AF372571; AF372572; EF122241; EF122242; EF122243; EF122244; EF122245; P51589 CP2J2_HUMAN Cytochrome P450 2J2 (EC CYP2J2 U37143; AF272142; AY426985; 1.14.14.1) (Arachidonic BC032594; acid epoxygenase) (CYPIIJ2) Q6VVX0 CP2R1_HUMAN Vitamin D 25-hydroxylase CYP2R1 AY323817; BC104907; BC104909; (EC 1.14.13.15) AY800276; (Cytochrome P450 2R1) Q96SQ9 CP2S1_HUMAN Cytochrome P450 2S1 (EC CYP2S1 AF335278; AY358603; AK027605; 1.14.14.1) (CYPIIS1) UNQ891/PRO1906 BC033691; Q7Z449 CP2U1_HUMAN Cytochrome P450 2U1 (EC CYP2U1 AY343323; CH471057; BC012027; 1.14.14.1) BC132767; BC136483; Q8TAV3 CP2W1_HUMAN Cytochrome P450 2W1 (EC CYP2W1 BC025761; 1.14.14.—) (CYPIIW1) Q9HB55 CP343_HUMAN Cytochrome P450 3A43 (EC CYP3A43 AF319634; AF337813; AF280107; 1.14.14.1) AF280108; AF280109; AF280110; AF280111; AY390423; AY390424; AY390425; AY390426; Q9NYL5 CP39A_HUMAN 24-hydroxycholesterol 7- CYP39A1 AF237982; AK292283; AL591242; alpha-hydroxylase AL035670; AL035670; AL591242; (Oxysterol 7-alpha- BC010358; hydroxylase) (EC 1.14.13.99) (Cytochrome P450 39A1) (hCYP39A1) P08684 CP3A4_HUMAN Cytochrome P450 3A4 CYP3A4 CYP3A3 D00003; M13785; M18907; M14096; (Albendazole X12387; J04449; AF182273; AF280107; monooxygenase) (EC AF209389; 1.14.13.32) (Albendazole sulfoxidase) (CYPIIIA3) (CYPIIIA4) (Cytochrome P450 3A3) (Cytochrome P45C HLp) (Cytochrome P450 NF-25) (Cytochrome P450-PCN1) (Nifedipine oxidase) (Quinine 3- monooxygenase) (EC 1.14.13.67) (Taurochenodeoxycholate 6-alpha-hydroxylase) (EC 1.14.13.97) P20815 CP3A5_HUMAN Cytochrome P450 3A5 (EC CYP3A5 J04813; AC005020; CH236956; 1.14.14.1) (CYPIIIA5) CH471091; BC033862; AF280107; (Cytochrome P450 HLp2) L35912; S74699; S74700; (Cytochrome P450-PCN3) P24462 CP3A7_HUMAN Cytochrome P450 3A7 (EC CYP3A7 D00408; AF280107; CH236956; 1.14.14.1) (CYPIIIA7) CH471091; BC067436; (Cytochrome P450-HFLA) Q9Y6A2 CP46A_HUMAN Cholesterol 24-hydroxylase CYP46A1 CYP46 AF094480; BC022539; (CH24H) (EC 1.14.13.98) (Cytochrome P450 46A1) Q02928 CP4AB_HUMAN Cytochrome P450 4A11 (20- CYP4A11 CYP4A2 L04751; D26481; S67580; S67581; hydroxyeicosatetraenoic AF525488; AY369778; AL731892; acid synthase) (20-HETE AL731892; BC041158; X71480; synthase) (CYP4AII) (CYPIVA11) (Cytochrome P- 450HK-omega) (Cytochrome P450HL-omega) (Fatty acid omega-hydroxylase) (Lauric acid omega-hydroxylase) (EC 1.14.15.3) Q5TCH4 CP4AM_HUMAN Cytochrome P450 4A22 CYP4A22 AF208532; AY280371; AY280372; (CYPIVA22) (Fatty acid AL135960; AL135960; BC148248; omega-hydroxylase) (Lauric acid omega-hydroxylase) (EC 1.14.15.3) P13584 CP481_HUMAN Cytochrome P450 4B1 (EC CYP4B1 J02871; X16699; AF491285; AY064485; 1.14.14.1) (CYPIVB1) AY064486; AY151048; DQ518907; (Cytochrome P450-HP) AL593856; AL356793; AL593856; AL356793; AL356793; AL593856; AL356793; AL593856; BC017758; P78329 CP4F2_HUMAN Leukotriene-B(4) omega- CYP4F2 D26480; U02388; AB015306; AK290790; hydroxylase 1 (EC AF467894; AC005336; BC067437; 1.14.13.30) (CYPIVF2) BC067439; BC067440; AF221943; (Cytochrome P450 4F2) (Cytochrome P450-LTB- omega) (Leukotriene-B(4) 20-monooxygenase 1) Q08477 CP4F3_HUMAN Leukotriene-B(4) omega- CYP4F3 LTB4H D12620; D12621; AB002454; AB002461; hydroxylase 2 (EC AF054821; AY792513; 1.14.13.30) (CYPIVF3) (Cytochrome P450 4F3) (Cytochrome P450-LTB- omega) (Leukotriene-B(4) 20-monooxygenase 2) P98187 CP4F8_HUMAN Cytochrome P450 4F8 (EC CYP4F8 AF133298; 1.14.14.1) (CYPIVF8) Q9HBI6 CP4F8_HUMAN Cytochrome P450 4F11 (EC CYP4F11 AF236085; AC005336; BC016853; 1.14.14.1) (CYPIVF11) Q9HCS2 CP4FC_HUMAN Cytochrome P450 4F12 (EC CYP4F12 AY008841; AB035130; AB035131; 1.14.14.1) (CYPIVF12) UNQ568/PRO1129 AY358977; AC004523; CH471106; Q6NT55 CP4FN_HUMAN Cytochrome P450 4F22 (EC CYP4F22 AK096820; BC069351; BC093894; 1.14.14.—) BC093896; Q6ZWL3 CP4V2_HUMAN Cytochrome P450 4V2 (EC CYP4V2 AY422002; AK122600; AK126473; 1.14.—.—) FJ440682; BC060857; Q8N118 CP4X1_HUMAN Cytochrome P450 4X1 (EC CYP4X1 AY358537; AK098065; BC028102; 1.14.14.1) (CYPIVX1) UNQ1929/PRO4404 Q86W10 CP4Z1_HUMAN Cytochrome P450 4Z1 (EC CYP4Z1 AY262056; AY358631; AK292175; 1.14.14.1) (CYPIVZ1) UNQ3060/PRO9882 AL450996; AL135960; AL135960; AL450996; Q8N1L4 CP4Z2_HUMAN Putative cytochrome P450 CYP4Z2P AY696295; AK097373; family member 4Z2 Q16350 CP51A_HUMAN Lanosterol 14-alpha CYP51A1 CYP51 U23942; D55653; U51692; U51684; demethylase (LDM) (EC U51685; U51686; U51687; U51688; 1.14.13.70) (CYPLI) U51689; U51690; U51691; AK314205; (Cytochrome P450 51A1) AK295932; AC000120; CH236949; (Cytochrome P450-14DM) CH471091; CH471091; BC032322; (Cytochrome P45014DM) (Cytochrome P450LI) (Sterol 14-alpha demethylase) P22680 CP7A1_HUMAN Cholesterol 7-alpha- CYP7A1 CYP7 X56088; M93133; BC101777; BC112184; monooxygenase (EC L13460; M89647; 1.14.13.17) (CYPVII (Cholesterol 7-alpha- hydroxylase) (Cytochrome P450 7A1) O75881 CP7B1_HUMAN 25-hydroxycholesterol 7- CYP7B1 AF029403; AF127090; AF176805; alpha-hydroxylase AF176800; AF176801; AF176802; (Oxysterol 7-alpha- AF176803; AF176804; CH471068; hydroxylase) (EC BC136574; 1.14.13.100) (Cytochrome P450 7B1) Q9UNU6 CP8B1_HUMAN 7-alpha-hydroxycholest-4- CYP8B1 CYP12 AF090318; AF090320; AK315330; en-3-one 12-alpha- BC067434; BC067441; BC067442; hydroxylase (EC BC067444; 1.14.13.95) (7-alpha- hydroxy-4-cholesten-3-one 12-alpha-hydroxylase) (CYPVIIIB1) (Cytochrome P450 8B1) (Sterol 12- alpha-hydroxylase) Q16647 PTGIS_HUMAN Prostacyclin synthase (EC PTGIS CYP8 D38145; AF297048; AF297049; 5.3.99.4) (Prostaglandin CYP8A1 AF297050; AF297051; AF297052; I2 synthase) AL118525; BC101809; BC101811; P24557 THAS_HUMAN Thromboxane-A synthase TBXAS1 M80647; D34625; L36085; L36075; (TXA synthase) (TXS) (EC CYP5 CYP5A1 L36076; L36077; L36078; L36079; 5.3.99.5) (Cytochrome L36080; L36081; L36082; L36083; P450 5A1) L36084; AF233615; AF233616; AF233617; AF233618; AF233619; AF233620; AF233621; AF233622; AF233623; AF233624; AF233625; BC041157; M74055; Cyto- Q6P9G0 CB5D1_HUMAN Cytochrome b5 domain- CYB5D1 AK057061; AK289520; CH471108; chrome containing protein 1 CH471108; CH471108; BC060779; b5 O431B9 CYB5B_HUMAN Cytochrome b5 type B CYB5B CYB5M AB009282; AK291576; BC004373; (Cytochrome b5 outer OMB5 BC014431; mitochondrial membrane isoform) P00167 CYB5_HUMAN Cytochrome b5 (Microsomal CYB5A CYB5 M22865; M60174; L39945; L39792; cytochrome b5 type A) L39941; L39942; L39943; L39944; (MCB5) CR456990; CH471117; BC015182; Q9UMX5 NENF_HUMAN Neudesin (Cell NENF CIR2 SPUF AB126219; AF173937; AY762102; immortalization-related AK223135; CH471100; BC008823; protein 2) (Neuron-derived neurotrophic factor) (Secreted protein of unknown function) (SPUF protein) Q8WUJ1 NEUFC_HUMAN Neuferricin (Cytochrome b5 CYB5D2 AK172844; AK313088; CH471108; domain-containing protein CH471108; BC020263; BC051697; 2) O00264 PGRC1_HUMAN Membrane-associated PGRMC1 HPR6.6 Y12711; BC034238; AJ249131; progesterone receptor PGRMC component 1 (mPR) O15173 PGRC2_HUMAN Membrane-associated PGRMC2 DG6 PMBP AJ002030; DQ496105; BC016692; progesterone receptor BC092478; component 2 (Progesterone membrane-binding protein) (Steroid receptor protein DG6) D6RFH4 D6RFH4_HUMAN Uncharacterized protein CYB5B Cyto- P08574 CY1_HUMAN Cytochrome c1, heme CYC1 M16597; J04444; CR541674; BT019798; chrome c protein, mitochondrial DQ300360; BC001006; BC015616; (Complex III subunit 4) BC020566; X06994; (Complex III subunit IV) (Cytochrome b-c1 complex subunit 4) (Ubiquinol- cytochrome-c reductase complex cytochrome c1 subunit) (Cytochrome c-1) P99999 CYC_HUMAN Cytochrome c CYCS CYC M22877; BT006946; AK311836; AL713681; AC007487; CH236948; CH471073; BC005299; BC008475; BC008477; BC009578; BC009579; BC009582; BC009587; BC009602; BC009607; BC014359; BC014361; BC015130; BC016006; BC021994; BC022330; BC067222; BC068464; BC070156; BC070346; BC071761; Cyto- Q5JTJ3 CA031_HUMAN Uncharacterized C1orf31 AL355472; AL355472; AL355472; chrome c protein C1orf31 BC025793; BC116455; oxidases P00403 COX2_HUMAN Cytochrome c oxidase MT-CO2 COII V00662; J01415; X15759; M25171; subunit 2 (Cytochrome c COXII MTCO2 D38112; U12690; U12691; U12692; oxidase polypeptirie II) U12693; U12694; AF004339; AY339402; AY339403; AY339404; AY339405; AY339406; AY339407; AY339408; AY339409; AY339410; AY339411; AY339412; AY339413; AY339414; AY339415; AY339416; AY339417; AY339418; AY339419; AY339420; AY339421; AY339422; AY339423; AY339424; AY339425; AY339426; AY339427; AY339428; AY339429; AY339430; AY339431; AY339432; AY339433; AY339434; AY339435; AY339436; AY339437; AY339438; AY339439; AY339440; AY339441; AY339442; AY339443; AY339444; AY339445; AY339446; AY339447; AY339448; AY339449; AY339450; AY339451; AY339452; AY339453; AY339454; AY339455; AY339456; AY339457; AY339458; AY339459; AY339460; AY339461; AY339462; AY339463; AY339464; AY339465; AY339466; AY339467; AY339468; AY339469; AY339470; AY339471; AY339472; AY339473; AY339474; AY339475; AY339476; AY339477; AY339478; AY339479; AY339480; AY339481; AY339482; AY339483; AY339484; AY339485; AY339486; AY339487; AY339488; AY339489; AY339490; AY339492; AY339493; AY339494; AY339495; AY339496; AY339498; AY339499; AY339500; AY339501; AY339502; AY339503; AY339504; AY339505; AY339506; AY339507; AY339508; AY339509; AY339510; AY339511; AY339512; AY339513; AY339514. P00414 COX3_HUMAN Cytochrome c oxidase MT-CO3 COIII J01415; V00662; DQ654394; DQ654395; subunit 3 (Cytochrome c COXIII MTCO3 DQ654396; DQ654397; DQ654398; oxidase polypeptide III) DQ654399; DQ654400; DQ654401; DQ654402; DQ654403; DQ654404; DQ654405; DQ654406; DQ654407; DQ654408; DQ654409; DQ654410; DQ654411; DQ654412; DQ654413; DQ654414; DQ654415; DQ654416; DQ654417; DQ654418; DQ654419; DQ654420; DQ654421; DQ654422; DQ654423; DQ654424; DQ654425; DQ654426; DQ654427; DQ654428; DQ654429; DQ654430; DQ654431; DQ654432; DQ654433; DQ654434; DQ654435; DQ654436; DQ654437; DQ654438; DQ654439; DQ654440; DQ654441; DQ654442; DQ654443; AF004341; P13073 COX41_HUMAN Cytochrome c oxidase COX4I1 COX4 M21575; M34600; X54802; U90915; subunit 4 isoform 1, AF005889; AF017115; AF042746; mitochondrial (Cytochrome AF042744; AF042745; BT019825; c oxidase polypeptide IV) AK311847; CH471114; CH471114; (Cytochrome c oxidase BC008704; BC021236; BC062437; subunit IV isoform 1) (COX IV-1) Q96KJ9 COX42_HUMAN Cytochrome c oxidase COX4I2 COX4L2 AF257180; AL117381, BC057779; subunit 4 isoform 2, mitochondrial (Cytochrome c oxidase subunit IV isoform 2) (COX IV-2) P20674 COX5A_HUMAN Cytochrome c oxidase COX5A M22760; DQ987236; DQ987237; subunit 5A, mitochondrial CR407649; CH471136; BC024240; (Cytochrome c oxidase polypeptide Va) P10606 COX5B_HUWlAN Cytochrome c oxidase COX5B M19961; M59250; BC006229; U41284; subunit 5B, mitochondrial (Cytochrome c oxidase polypeptide Vb) P09669 COX6C_HUMAN Cytochrome c oxidase COX6C X13238; AF067637; AF067636; subunit 6C (Cytochrome BT007007; AK311791; CH471060; c oxidase polypeptide VIc) BC000187; P24311 COX7B_HUMAN Cytochrome c oxidase COX7B Z14244; BT009767; CR450332; subunit 7B, mitochondrial CR542124; AK311879; AL356235; (Cytochrome c oxidase CH471104; BC018386; polypeptide VIIb) P15954 COX7C_HUMAN Cytochrome c oxidase COX7C X16560; AF067639; AF067638; subunit 7C, mitochondrial BT007098; BC001005; BC007498; (Cytochrome c oxidase polypeptide VIIc) O14548 COX7R_HUMAN Cytochrome c oxidase COX7A2L COX7AR AB007618; AF127788; AY007643; subunit 7A-related COX7RP BT007371; BC005251; BC007095; protein, mitochondrial (COX7a-related protein) (Cytochrome c oxidase subunit VIIa-related protein) (EB1) O60397 COX7S_HUMAN Putative Cytochrome c COX7A2P2 COX7A3 AC004544; oxidase subunit 7A3, COX7AL2 COX7AP2 mitochondrial (Cytochrome c oxidase subunit VIIa 3) P10176 COX8A_HUMAN Cytochrome c oxidase COX8A COX8 J04823; BC063025; subunit 8A, mitochondrial COX8L (Cytochrome c oxidase polypeptide VIII- liver/heart) (Cytochrome c oxidase subunit 8-2) Q7Z4L0 COX8C_HUMAN Cytochrome c oxidase COX8C AY161004; BC101125; BC101126; subunit 8C, mitochondrial (Cytochrome c oxidase polypeptide 8 isoform 3) (Cytochrome c oxidase polypeptide VIII isoform 3) (COX VIII-3) (Cytochrome c oxidase subunit 8-3) P12074 CX6A1_HUMAN Cytochrome c oxidase COX6A1 COX6AL AK312009; AL021546; BC007723; subunit 6A1, mitochondrial BC070186; BC107861; X15341; (Cytochrome c oxidase polypeptidc VIa-liver) (Cytochrome c oxidase subunit VIA-liver) (COX VIa-L) Q02221 CX6A2_HUMAN Cytochrome c oxidase COX6A2 COX6A M83308; U66875; BC029818; subunit 6A2, mitochondrial COX6AH (Cytochrome c oxidase polypeptide VIa-heart) (COXVIAH) (Cytochrome c oxidase subunit VIA- muscle) (COX VIa-M) P14854 CX6B1_HUMAN Cytochrome c oxidase COX6B1 COX6B X13923; X54473; AK312140; BT006945; subunit 6B1 (Cytochrome c CR456789; CR542137; AC002115; oxidase subunit VIb BC001015; BC002478; X58139; isoform 1) (COX VIb-1) Q6YFQ2 CX6B2_HUMAN Cytochrome c oxidase COX6B2 AY152398; AK057427; BC026123; subunit 6B2 (Cancer/testis BC100899; BC100900; BC100901; antigen 59) (CT59) BC100902; (Cytochrome c oxidase subunit VIb isoform 2) (COX VIb-2) (Cytochrome c oxidase subunit VIb, testis-specific isoform) P24310 CX7A1_HUMAN Cytochrome c oxidase COX7A1 COX7AH M83186; U81524; AC002984; AF037372; subunit 7A1, mitochondrial AF127789; AD001527; BC002757; (Cytochrome c oxidase subunit VIIa-heart) (Cytochrome c oxidase subunit VIIa-H) (Cytochrome c oxidase subunit VIIa-muscle) (Cytochrome c oxidase subunit VIIa-M) P14406 CX7A2_HUMAN Cytochrome c oxidase COX7A2 COX7AL X15822; AF134406; CR407646; subunit 7A2, mitochondrial CR542125; AK312154; AL080250; (Cytochrome c oxidase BC101826; BC101828; subunit VIIa-liver/heart) (Cytochrome c oxidase subunit VIIa-L) (Cytochrome c oxidase subunit VIIaL) Q8TF08 CX7B2_HUMAN Cytochrome c oxidase COX7B2 AF125109; BC035923; BC107855; subunit 7B2, mitochondrial (Cytochrome c oxidase polypeptide VIIb2) Prosta- P23219 PGH1_HUMAN Prostaglandin G/H synthase PTGS1 COX1 M31822; M31812; M31813; M31814; glandin 1 (EC 1.14.99.1) M31815; M31816; M31817; M31818; G/H (Cyclooxygenase-1) (COX-1) M31819; M31820; M31821; M59979; synthase (Prostaglandin H2 S78220; S36219; S36271; AF440204; synthase 1) (PGH synthase AK290022; AY449688; AL162424; 1) (PGHS-1) (PHS 1) AL359636; AL162424; AL359636; (Prostaglandin- AL162424; AL359636; AL162424; endoperoxide synthase 1) AL359636; AL162424; CH471090; BC029840; P35354 PGH2_HUMAN Prostaglandin G/H synthase PTGS2 COX2 L15326; M90100; D28235; U04636; 2 (EC 1.14.99.1) AY462100; AY229989; AY382629; (Cyclooxygenase-2) (COX-2) AK292167; AL033533; CH471067; (PHS II) (Prostaglandin BC013734; H2 synthase 2) (PGH synthase 2) (PGHS-2) (Prostaglandin- endoperoxide synthase 2) Catalases P04040 CATA_HUMAN Catalase (EC 1.11.1.6) CAT X04085; X04086; X04087; X04088; X04089; X04090; X04091; X04092; X04093; X04094; X04095; X04096; X04076; AY028632; AK291585; AK315350; AY545477; AL035079; CH471064; CH471064; BC110398; BC112217; BC112219; L13609; K02400; B4DWK8 B4DWK8_HUMAN Catalase (EC 1.11.1.6) AK301577; Perox- Q9NRD9 DUOX1_HUMAN Dual oxidase 1 (EC DUOX1 DUOX AF230495; AF213465; AK172859; idases 1.11.1.—) (EC 1.6.3.1) LNOX1 THOX1 BC114628; (Large NOX 1) (Long NOX 1) (NADPH thyroid oxidase 1) (Thyroid oxidase 1) Q9NRD8 DUOX2_HUMAN Dual oxidase 2 (EC DUOX2 LNOX2 AF230496; AF267981; AF181972; 1.11.1.—) (EC 1.6.3.1) THOX2 (Large NOX 2) (Long NOX 2) (NADH/NADPH thyroid oxidase p138-tox) (NADPH oxidase/peroxidase DUOX2) (NADPH thyroid oxidase 2) (Thyroid oxidase 2) (p138 thyroid oxidase) P11678 PERE_HUMAN Eosinophil peroxidase EPX EPER EPO EPP M29913; M29904; M29905; M29906; (EPO) (EC 1.11.1.7) M29907; M29908; M29909; M29910; [Cleaved into: Eosinophil M29911; M29912; DQ054598; X14346; peroxidase light chain; Eosinophil peroxidase heavy chain] P22079 PERL_HUMAN Lactoperoxidase (LPO) (EC LPO SAPX U39573; AY324876; BC107166; 1.11.1.7) (Salivary BC107167; M58151; peroxidase) (SPO) P05164 PERM_HUMAN Myeloperoxidase (MPO) (EC MPO J02694; M17176; M17170; M17171; 1.11.1.7) [Cleaved into: M17172; M17173; M17174; M17175; 89 kDa myeloperoxidase; X04876; M19507; M19508; M19508; 84 kDa myeloperoxidase; X15377; S56200; DQ088846; CH471109; Myeloperoxidase light BC130476; D14466; chain; Myeloperoxidase heavy chain] P07202 PERT_HUMAN Thyroid peroxidase (TPO) TPO J02969; J02970; Y00406; M25715; (EC 1.11.1.8) M25702; M25703; M25704; M25705; M25706; M25707; M25708; M25709; M25710; M25711; M25712; M25713; M25714; X17358; M17755; AF439430; AF533528; AY136822; AF533529; AF533530; AF533531; M55702; M55702; A1KZ92 PXDNL_HUMAN Peroxidasin-like protein PXDNL VPO2 EU170240; AY877349; AK058200; (EC 1.11.1.7) (Cardiac AK131524; CH471068; peroxidase) (Vascular peroxidase 2) Q92626 PXDN_HUMAN Peroxidasin homolog (EC PXDN KIAA0230 AF200348; EF090903; D86983; 1.11.1.7) (Melanoma- MG50 PRG2 VPO CH471053; CH471053; BC098579; associated antigen MG50) VPO1 (Vascular peroxidase 1) (p53-responsive gene 2 protein) Trans- P02787 TRFE_HUMAN Serotransferrin TF PRO1400 M12530; M17611; M17610; M17614; ferrins (Transferrin) (Beta-1 M17612; M17613; S95936; AF288144; metal-binding AF294270; AF294271; AF288139; globulin) (Siderophilin) AF288140; AF288141; AF288142; AF288143; AY308797; DQ525716; CH471052; BC059367; M21569; M15673; M21570; X04600; AJ252279; M11372; M11361; M11362; M11363; M11364; M11365; M11366; M11367; M11368; M11369; M11370; M11371; AF118063; M12525; U88581; AF058327; M26641; P02788 TRFL_HUMAN Lactotransferrin LTF LF X53961; U07643; M93150; M83202; (Lactoferrin) (EC M83205; M73700; AF332168; 3.4.21.—) (Talalactoferrin) AY137470; AY165046; AY178998; [Cleaved into: Kaliocin-1; BC015822; BC015823; BC022347; Lactoferroxin-A; S52659; X52941; M18642; U95626; Lactoferroxin-B; Lactoferroxin-C] P08582 TRFM_HUMAN Melanotransferrin MFI2 MAP97 M12154; BC001875; BC002623; (Melanoma-associated BC007550; BC071910; antigen p97) (CD antigen CD228) Nitric P29475 NOS1_HUMAN Nitric oxide synthase, NOS1 U17327; U17326; U17299; U17300; Oxide brain (EC 1.14.13.39) U17301; U17302; U17303; U17304; Synthases (Constitutive NOS) (NC- U17305; U17307; U17308; U17309; NOS) (NOS type I) (Neuronal U17310; U17311; U17312; U17313; NOS) (N-NOS) (nNOS) U17314; U17315; U17316; U17317; (Peptidyl-cysteine S- U17318; U17319; U17320; U17321; nitrosylase NOS1) (bNOS) U17322; U17323; U17324; U17325; D16408; L02881; U31466; AY445095; P35228 NOS2_HUMAN Nitric oxide synthase, NOS2 NOS2A L24553; L09210; X73029; U05810; indudble (EC 1.14.13.39) U31511; D26525; U20141; AF068236; (Hepatocyte NOS) (HEP-NOS) AB022318; DQ060518; EU332854; (Inducible NO synthase) BC130283; BC144126; S75615; (Inducible NOS) (iNOS) (NOS type II) (Peptidyl- cysteine S-nitrosylase NOS2) P29474 NOS3_HUMAN Nitric oxide synthase, NOS3 M93718; M95296; L10709; L10693; endothelial (EC L10694; L10695; L10696; L10697; 1.14.13.39) (Constitutive L10698; L10699; L10700; L10701; NOS) (cNOS) (EC-NOS) L10702; L10703; L10704; L10705; (Endothelial NOS) (eNOS) L10706; L10707; L10708; L26914; (NOS type III) (NOSIII) X76303; X76304; X76305; X76306; X76307; X76308; X76309; X76310; X76311; X76312; X76313; X76314; X76315; X76316; D26607; AF400594; AK292928; AK315213; AK223636; AF519768; EU332855; CH471173; BC063294; BC069465; L23210; S80791; O75713 O75713_HUMAN Neuronal nitric-oxide nNOSmu AJ004918 synthase isoform mu (EC 1.14.13.39) (Fragment) Heme- P09601 HMOX1_HUMAN Heme oxygenase 1 (HO-1) HMOX1 HO HO1 X06985; CR456505; AY460337; Z82244; oxy- (EC 1.14.99.3) M23041; X14782; genases P30519 HMOX2_HUMAN Heme oxygenase 2 (HO-2) HMOX2 HO2 D21243; S34389; BT019788; AY771350; (EC 1.14.99.3) CH471112; CH471112; BC002396; AF051306;

TABLE 2 Lignin Peroxidases UniProtKB/ Swiss-Prot EMBL Accession Entry name Protein names Gene names Accession No. Protein family Organism P28313 PER_ARTRA Peroxidase (EC D63792; Peroxidase family, Arthromyces ramosus 1.11.1.7) Ligninase subfamily P28314 PER_COPCI Peroxidase (EC CIP1 X69457; X70789; Peroxidase family, Coprinopsis cinerea 1.11.1.7) Ligninase subfamily (Inky cap fungus) (Hormographiella aspergillata) A8NK72 PER_COPC7 Peroxidase (EC CIP1 AACS02000010; Peroxidase family, Coprinopsis cinerea 1.11.1.7) CC1G_02104 Ligninase subfamily (strain Okayama-7/130/ FGSC 9003) (Inky cap fungus) (Hormographiella aspergillata) P49012 LIG2_PHACH Ligninase LG2 (EC GLG2 LIP2 M74229; M92644; Peroxidase family, Phanerochaete 1.11.1.14) Ligninase subfamily chrysosporium (White- (Diarylpropane rot fungus) peroxidase) (Lignin (Sporotrichum peroxidase) pruinosum) P21764 LIG3_PHACH Ligninase LG3 (EC GLG3 LIP X51590; Peroxidase family, Phanerochaete 1.11.1.14) Ligninase subfamily chrysosporium (White- (Diarylpropane rot fungus) peroxidase) (Lignin (Sporotrichum peroxidase) pruinosum) P11542 LIG4_PHACH Ligninase H2 (EC GLG4 LIP2 X15599; M18743; Peroxidase family, Phanerochaete 1.11.1.14) Ligninase subfamily chrysosporium (White- (Diarylpropane rot fungus) peroxidase) (LG4) (Sporotrichum (Lignin peroxidase) pruinosum) P11543 LIG5_PHACH Ligninase LG5 (EC GLG5 LIP6 M18794; X55343; M63496; Peroxidase family, Phanerochaete 1.11.1.14) Ligninase subfamily chrysosporium (White- (Diarylpropane rot fungus) peroxidase) (Lignin (Sporotrichum peroxidase) pruinosum) P50622 LIG6_PHACH Ligninase LG6 (EC GLG6 M80213; Peroxidase family, Phanerochaete 1.11.1.14) Ligninase subfamily chrysosporium (White- (Diarylpropane rot fungus) peroxidase) (Lignin (Sporotrichum peroxidase) pruinosum) P06181 LIG8_PHACH Ligninase H8 (EC LPOA M37701; Y00262; M27401; Peroxidase family, Phanerochaete 1.11.1.14) M27884; Ligninase subfamily chrysosporium (White- (Diarylpropane rot fungus) peroxidase) (Lignin (Sporotrichum peroxidase) pruinosum) P31837 LIGA_PHACH Ligninase A (EC LIPA LPOB X54257; M37701; Peroxidase family, Phanerochaete 1.11.1.14) Ligninase subfamily chrysosporium (White- (Diarylpropane rot fungus) peroxidase) (Lignin (Sporotrichum peroxidase) pruinosum) P31838 LIGB_PHACH Ligninase B (EC LIPB X54257; Peroxidase family, Phanerochaete 1.11.1.14) Ligninase subfamily chrysosporium (White- (Diarylpropane rot fungus) peroxidase) (Lignin (Sporotrichum peroxidase) pruinosum) Q02567 PEM1_PHACH Manganese MNP1 M60672; M77513; J04624; Peroxidase family, Phanerochaete peroxidase 1 (MnP-1) Ligninase subfamily chrysosporium (White- (MnP1) rot fungus) (EC 1.11.1.13) (Sporotrichum (Manganese pruinosum) peroxidase isozyme 1) (Peroxidase manganese-dependent 1) (Peroxidase manganese-dependent I) P78733 PEM3_PHACH Manganese U10306; Peroxidase family, Phanerochaete peroxidase H3 (EC Ligninase subfamily chrysosporium (White- 1.11.1.13) (Peroxidase rot fungus) manganese-dependent (Sporotrichum H3) pruinosum) P19136 PEM4_PHACH Manganese J04980; Peroxidase family, Phanerochaete peroxidase H4 (EC Ligninase subfamily chrysosporium (White- 1.11.1.13) (MP-I) rot fungus) (Peroxidase (Sporotrichum manganese-dependent pruinosum) H4) Q9URB1 PEM5_PHACH Manganese Peroxidase family, Phanerochaete peroxidase H5 (EC Ligninase subfamily chrysosporium (White- 1.11.1.13) (Peroxidase rot fungus) manganese-dependent (Sporotrichum H5) (Fragment) pruinosum) P20010 LIG_PHLRA Ligninase-3 (EC Peroxidase family, Phlebia radiata (White- 1.11.1.14) Ligninase subfamily rot fungus) (Diarylpropane peroxidase) (Lignin peroxidase) (Ligninase III) Q70LM3 PEM2_PHLRA Manganese mnp2 AJ315701; AJ566199; Peroxidase family, Phlebia radiata (White- peroxidase 2 (MnP2) Ligninase subfamily rot fungus) (EC 1.11.1.13) (Manganese peroxidase isozyme 2) Q96TS6 PEM3_PHLRA Manganese mnp3 AJ310930; AJ566200; Peroxidase family, Phlebia radiata (White- peroxidase 3 (MnP3) Ligninase subfamily rot fungus) (EC 1.11.1.13) (Manganese peroxidase isozyme 3) Q9UR19 VPL1_PLEER Versatile peroxidase vpl1 AF007221; AF007223; Peroxidase family, Pleurotus eryngii VPL1 (EC 1.11.1.16) Ligninase subfamily (Boletus of the steppes) (Versatile liquid phase peroxidase 1) O94753 VPL2_PLEER Versatile peroxidase vpl2 AF007222; AF007224; Peroxidase family, Pleurotus eryngii VPL2 (EC 1.11.1.16) Ligninase subfamily (Boletus of the steppes) (Versatile liquid phase peroxidase 2) Q9UVP6 VPS1_PLEER Versatile peroxidase vps1 ps1 AF175710; Peroxidase family, Pleurotus eryngii VPS1 (EC 1.11.1.16) Ligninase subfamily (Boletus of the steppes) (Versatile solid phase peroxidase 1) C0IW58 LNP_TAICA Low-redox potential LnP EU289404; EU526903; Peroxidase family, Taiwanofungus peroxidase (EC Ligninase subfamily camphoratus (Poroid 1.11.1.7) (Putative brown-rot fungus) ligninolytic (Antrodia camphorata) peroxidase) P20011 LIGA_TRAVE Ligninase A (EC Peroxidase family, Trametes versicolor 1.11.1.14) Ligninase subfamily (White-rot fungus) (Diarylpropane (Coriolus versicolor) peroxidase) (Lignin peroxidase) (Fragment) P20012 LIGB_TRAVE Ligninase B (EC Peroxidase family, Trametes versicolor 1.11.1.14) Ligninase subfamily (White-rot fungus) (Diarylpropane (Coriolus versicolor) peroxidase) (Lignin peroxidase) (Fragment) P20013 LIGC_TRAVE Ligninase C (EC M64993; Peroxidase family, Trametes versicolor 1.11.1.14) Ligninase subfamily (White-rot fungus) (Diarylpropane (Coriolus versicolor) peroxidase) (Lignin peroxidase) (excitation at 397 nm) and resonance Raman spectroscopy. As demonstrated herein, recombinant heme-binding proteins generated using the methods of the present invention show no evidence of free base porphryin incorporation.

The recombinant ferrochelatase protein or polypeptide used in the methods of the present invention includes any recombinant ferrochelatase, or polypeptide thereof that is capable of catalyzing the insertion of ferrous iron into protoporphyrin IX to form protoheme (i.e., enzyme classification (EC) no. 4.99.1.1). Well over 400 ferrochelatases from both prokaryotes and eukaryotes have been characterized and are known in the art (see e.g., UniProtKB, (GenBank). Table 3 below provides a listing of 429 known ferrochelatase enzymes from the UniProtKB database identified by UniProtKB/Swiss-Pro Accession number, protein name, gene name, organism, EC number, and EMBL accession number. Each of the ferrochelatases listed in Table 3 is suitable for use in the present invention. The UniProtKB/Swiss Prot and EMBL accession numbers, along with the corresponding amino acid and nucleotide sequence information for each entry in Table 3 is hereby incorporated by reference. Selection of an appropriate ferrochelatase to use when carrying out the methods of the present invention is based on the recombinant heme-binding protein being produced and/or the chosen expression system.

It is to be understood that the present invention contemplates the use of any bacterial, archaeal, fungal, plant, and animal ferrochelatase known in the art. The present invention also contemplates the use of polypeptide fragments of ferrochelatase which retain iron insertion activity (i.e., suitable proteins or polypeptides have an enzyme classification of 4.99.1.1). Methods of analyzing and measuring the enzyme activity of ferrochelatase are well known in the art (see e.g., Miyamoto et al., “Overproduction, Purification, and Characterization of Ferrochelatase from Escherichia coli,” J. Biochem. 115:545-551 (1994) and Taketani et al., “Rat Liver Ferrochelatase,” J. Biol. Chem., 256(24):12748-53 (1981), which are hereby incorporated by reference in their entirety). Therefore, suitable ferrochelatase proteins or polypeptides not identified in Table 3 can be readily identified by one of skill in the art using these enzyme activity assays.

TABLE 3 Characterized Ferrochelatase Enzymes UniProKB/ SwissProt Accession No. Entry Name Gene Names Organism EC Number Protein Names EMBL Accession No. P42043 HEMH1_ARATH HEM15 At5g26030 Arabidopsis thaliana (Mouse-ear 4.99.1.1 Ferrochelatase-1, chloroplastic/mitochondrial (EC X73417; Y13382; AF149413; T1N24.17 cress) 4.99.1.1) (Ferrochelatase I) (Heme synthase 1) (Protoheme ferro-lyase 1) Q81U22 HEMH1_BACAN hemH1 hemH-1 Bacillus anthracis 4.99.1.1 Ferrochelatase 1 (EC 4.99.1.1) (Heme synthase 1) AE016879; AE017334; AE017225; BA_1071 GBAA_1071 (Protoheme ferro-lyase 1) BAS1000 Q73C98 HEMH1_BACC1 hemH1 BCE_1168 Bacillus cereus (strain ATCC 10987) 4.99.1.1 Ferrochelatase 1 (EC 4.99.1.1) (Heme synthase 1) AE017194; (Protoheme ferro-lyase 1) Q81GW5 HEMH1_BACCR hemH1 BC_1069 Bacillus cereus (strain ATCC 14579/ 4.99.1.1 Ferrochelatase 1 (EC 4.99.1.1) (Heme synthase 1) AE016877; DSM 31) (Protoheme ferro-lyase 1) Q63ES4 HEMH1_BACCZ hemH1 BCE33L0987 Bacillus cereus (strain ZK/E33L) 4.99.1.1 Ferrochelatase 1 (EC 4.99.1.1) (Heme synthase 1) CP000001; (Protoheme ferro-lyase 1) Q6HM97 HEMH1_BACHK hemH1 BT9727_0985 Bacillus thuringiensis subsp. 4.99.1.1 Ferrochelatase 1 (EC 4.99.1.1) (Heme synthase 1) AE017355; konkukian (Protoheme ferro-lyase 1) Q69TB1 HEMH1_ORYSJ Os09g0297000 Oryza sativa subsp. japonica (Rice) 4.99.1.1 Ferrochelatase-1, chloroplastic (EC 4.99.1.1) AP004756; AP008215; AK068174; LOC_Os09g12560 (Ferrochelatase I) (Heme synthase 1) (Protoheme P0592C05.24 ferro-lyase 1) Q8EFF4 HEMH1_SHEON hemH1 hemH-1 Shewanella oneidensis 4.99.1.1 Ferrochelatase 1 (EC 4.99.1.1) (Heme synthase 1) AE014299; SO_2019 (Protoheme ferro-lyase 1) O04921 HEMH2_ARATH At2g30390 T06B20.24 Arabidopsis thaliana (Mouse-ear 4.99.1.1 Ferrochelatase-2, chloroplastic (EC 4.99.1.1) Y13156; U93215; AC002338; T9D9.1 cress) (Ferrochelatase II) (Heme synthase 2) (Protoheme BT000465; BT008877; ferro-lyase 2) Q81TU9 HEMH2_BACAN hemH2 hemH-2 Bacillus anthracis 4.99.1.1 Ferrochelatase 2 (EC 4.99.1.1) (Heme synthase 2) AE016879; AE017334; AE017225; BA_1158 GBAA_1158 (Protoheme ferro-lyase 2) BAS1075 Q73C08 HEMH2_BACC1 hemH2 BCE_1260 Bacillus cereus (strain ATCC 10987) 4.99.1.1 Ferrochelatase 2 (EC 4.99.1.1) (Heme synthase 2) AE017194; (Protoheme ferro-lyase 2) Q81GN7 HEMH2_BACCR hemH2 BC_1154 Bacillus cereus (strain ATCC 14579/ 4.99.1.1 Ferrochelatase 2 (EC 4.99.1.1) (Heme synthase 2) AE016877; DSM 31) (Protoheme ferro-lyase 2) Q63EK7 HEMH2_BACCZ hemH2 BCE33L1054 Bacillus cereus (strain ZK/E33L) 4.99.1.1 Ferrochelatase 2 (EC 4.99.1.1) (Heme synthase 2) CP000001; (Protoheme ferro-lyase 2) Q6HM28 HEMH2_BACHK hemH2 BT9727_1056 Bacillus thuringiensis subsp. 4.99.1.1 Ferrochelatase 2 (EC 4.99.1.1) (Heme synthase 2) AE017355; konkukian (Protoheme ferro-lyase 2) Q0DIV0 HEMH2_ORYSJ Os05g0361200 Oryza sativa subsp. japonica (Rice) 4.99.1.1 Ferrochelatase-2, chloroplastic (EC 4.99.1.1) AC134348; AC135929; AP008211; LOC_Os05g29760 (Ferrochelatase II) (Heme synthase 2) (Protoheme AK073873; P0530H10.9 ferro-lyase 2) P0692D12.2 Q8EBZ7 HEMH2_SHEON hemH2 hemH-2 Shewanella oneidensis 4.99.1.1 Ferrochelatase 2 (EC 4.99.1.1) (Heme synthase 2) AE014299; SO_3348 (Protoheme ferro-lyase 2) Q6F7N0 HEMH_ACIAD hemH ACIAD3255 Acinetobacter sp. (strain ADP1) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CR543861; (Protoheme ferro-lyase) B7H0X1 HEMH_ACIB3 hemH ABBFA_003155 Acinetobacter baumannii (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001172; AB307-0294) (Protoheme ferro-lyase) B7I4A8 HEMH_ACIB5 hemH AB57_0460 Acinetobacter baumannii (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001182; AB0057) (Protoheme ferro-lyase) B2I2R9 HEMH_ACIBC hemH ACICU_00392 Acinetobacter baumannii (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000863; ACICU) (Protoheme ferro-lyase) B0VLE0 HEMH_ACIBS hemH ABSDF3128 Acinetobacter baumannii (strain SDF) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CU468230; (Protoheme ferro-lyase) A3M1P7 HEMH_ACIBT hemH A1S_0382 Acinetobacter baumannii (strain ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000521; 17978/NCDC KC 755) (Protoheme ferro-lyase) B0VEC9 HEMH_ACIBY hemH ABAYE3393 Acinetobacter baumannii (strain AYE) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CU459141; (Protoheme ferro-lyase) C1F1C7 HEMH_ACIC5 hemH ACP_2433 Acidobacterium capsulatum (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001472; ATCC 51196/DSM 11244/JCM (Protoheme ferro-lyase) 7670) A3N3M9 HEMH_ACTP2 hemH APL_1937 Actinobacillus pleuropneumoniae 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000569; serotype 5b (strain L20) (Protoheme ferro-lyase) B3H307 HEMH_ACTP7 hemH APP7_2025 Actinobacillus pleuropneumoniae 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001091; serotype 7 (strain AP76) (Protoheme ferro-lyase) B0BTL7 HEMH_ACTPJ hemH APJL_1983 Actinobacillus pleuropneumoniae 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000687; serotype 3 (strain JL03) (Protoheme ferro-lyase) A0KL51 HEMH_AERHH hemH AHA_2488 Aeromonas hydrophila subsp. 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000462; hydrophila (strain ATCC 7966/NCIB (Protoheme ferro-lyase) 9240) Q8U9F7 HEMH_AGRT5 hemH Atu3771 Agrobacterium tumefaciens (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE007870; AGR_L_2129 C58/ATCC 33970) (Protoheme ferro-lyase) B9JS40 HEMH_AGRVS hemH Avi_3712 Agrobacterium vitis (strain S4/ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000633; BAA-846) (Rhizobium vitis (strain S4)) (Protoheme ferro-lyase) Q0VSV6 HEMH_ALCBS hemH ABO_0294 Alcanivorax borkumensis (strain SK2/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AM286690; ATCC 700651/DSM 11573) (Protoheme ferro-lyase) B6EHK1 HEMH_ALISL hemH VSAL_I0816 Alilvibrio salmonicida (strain LFI1238) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) FM178379; (Vibrio salmonicida (strain LFI1238)) (Protoheme ferro-lyase) B4S1G4 HEMH_ALTMD hemH MADE_00654 Alteromonas macieodii (strain DSM 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001103; 17117/Deep ecotype) (Protoheme ferro-lyase) Q3MCT9 HEMH_ANAVT hemH Ava_1574 Anabaena variabilis (strain ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000117; 29413/PCC 7937) (Protoheme ferro-lyase) B7GF12 HEMH_ANOFW hemH Aflv_2279 Anoxybacillus flavithermus (strain DSM 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000922; 21510/WK1) (Protoheme ferro-lyase) O67083 HEMH_AQUAE hemH aq_948 Aquifex aeolicus 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE000657; (Protoheme ferro-lyase) Q5P1H1 HEMH_AROAE hemH AZOSEA27180 Aromatoleum aromaticum (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CR555306; ebA4802 EbN1) (Azoarcus sp. (strain EbN1)) (Protoheme ferro-lyase) A1K8P4 HEMH_AZOSB hemH azo2582 Azoarcus sp. (strain BH72) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AM406670; (Protoheme ferro-lyase) C1DEU1 HEMH_AZOVD hemH Avin_41370 Azotobacter vinelandii (strain DJ/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001157; ATCC BAA-1303) (Protoheme ferro-lyase) Q9KDK9 HEMH_BACHD hemH BH1203 Bacillus halodurans 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BA000004; (Protoheme ferro-lyase) A8FBM6 HEMH_BACP2 hemH BPUM_0959 Bacillus pumilus (strain SAFR-032) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000813; (Protoheme ferro-lyase) Q5WHT1 HEMH_BACSK hemH ABC1539 Bacillus clausii (strain KSM-K16) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AP006627; (Protoheme ferro-lyase) P32396 HEMH_BACSU hemH hemF BSU10130 Bacillus subtilis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) M97208; Y14083; AL009126; (Protoheme ferro-lyase) A9VI99 HEMH_BACWK hemH Bacillus weihenstephanensis (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000903; BcerKBAB4_0990 KBAB4) (Protoheme ferro-lyase) Q6MHT3 HEMH_BDEBA hemH Bd3456 Bdellovibrio bacteriovorus 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX842655; (Protoheme ferro-lyase) Q7WGI0 HEMH_BORBR hemH BB3938 Bordetella bronchiseptica (Alcaligenes 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX640449; bronchisepticus) (Protoheme ferro-lyase) Q7W515 HEMH_BORPA hemH BPP3489 Bordetella parapertussis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX640433; (Protoheme ferro-lyase) Q7VVX8 HEMH_BORPE hemH BP2503 Bordetella pertussis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX640418; (Protoheme ferro-lyase) P22600 HEMH_BOVIN FECH Bos taurus (Bovine) 4.99.1.1 Ferrochelatase, mitochondrial (EC 4.99.1.1) (Heme L34173; synthase) (Protoheme ferro-lyase) P28602 HEMH_BRAJA hemH bll7752 Bradyrhizobium japonicum 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) M92427; BA000040; (Protoheme ferro-lyase) A5EBP8 HEMH_BRASB hemH BBta_1357 Bradyrhizobium sp. (strain BTAi1/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000494; ATCC BAA-1182) (Protoheme ferro-lyase) B2SCP3 HEMH_BRUA1 hemH BAbS19_II00700 Brucella abortus (strain S19) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000888; (Protoheme ferro-lyase) Q2YIS9 HEMH_BRUA2 hemH BAB2_0075 Brucella abortus (strain 2308) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AM040265; (Protoheme ferro-lyase) P0A3D8 HEMH_BRUAB hemH BruAb2_0076 Brucella abortus 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AF358663; AY027659; AE017224; (Protoheme ferro-lyase) A9MDJ3 HEMH_BRUC2 hemH BCAN_B0079 Brucella canis (strain ATCC 23365/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000873; NCTC 10854) (Protoheme ferro-lyase) C0RK28 HEMH_BRUMB hemH BMEA_B0080 Brucella melitensis biotype 2 (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001489; ATCC 23457) (Protoheme ferro-lyase) P0A3D6 HEMH_BRUME hemH BMEII0018 Brucella melitensis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE008918; (Protoheme ferro-lyase) A5VTJ7 HEMH_BRUO2 hemH BOV_A0071 Brucella ovis (strain ATCC 25840/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000709; 63/290/NCTC 10512) (Protoheme ferro-lyase) A9WXE2 HEMH_BRUSI hemH BSUIS_B0081 Brucella suis (strain ATCC 23445/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000912; NCTC 10510) (Protoheme ferro-lyase) P0A3D7 HEMH_BRUSU hemH BRA0076 Brucella suis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE014292; (Protoheme ferro-lyase) B1YTJ5 HEMH_BURA4 hemH Burkholderia ambifaria (strain MC40-6) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001025; BamMC406_0664 (Protoheme ferro-lyase) Q1BYX9 HEMH_BURCA hemH Bcen_0262 Burkholderia cenocepacia (strain AU 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000378; 1054) (Protoheme ferro-lyase) B1JW14 HEMH_BURCC hemH Bcenmc03_0716 Burkholderia cenocepacia (strain MC0- 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000958; 3) (Protoheme ferro-lyase) A0K4S2 HEMH_BURCH hemH Bcen2424_0746 Burkholderia cenocepacia (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000458; HI2424) (Protoheme ferro-lyase) Q0BI24 HEMH_BURCM hemH Bamb_0640 Burkholderia ambifaria (strain ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000440; BAA-244/AMMD) (Burkholderia (Protoheme ferro-lyase) cepacia (strain AMMD)) A3MNA4 HEMH_BURM7 hemH BMA10247_2211 Burkholderia mallei (strain NCTC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000548; 10247) (Protoheme ferro-lyase) A2S570 HEMH_BURM9 hemH Burkholderia mallei (strain NCTC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000546; BMA10229_A1104 10229) (Protoheme ferro-lyase) Q62HD1 HEMH_BURMA hemH BMA2330 Burkholderia mallei (Pseudomonas 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000010; mallei) (Protoheme ferro-lyase) A1V0U1 HEMH_BURMS hemH Burkholderia mallei (strain SAVP1) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000526; BMASAVP1_A0495 (Protoheme ferro-lyase) A3NYY2 HEMH_BURP0 hemH Burkholderia pseudomallei (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000572; BURPS1106A_3316 1106a) (Protoheme ferro-lyase) Q3JP06 HEMH_BURP1 hemH Burkholderia pseudomallei (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000124; BURPS1710b_3326 1710b) (Protoheme ferro-lyase) A3ND73 HEMH_BURP6 hemH Burkholderia pseudomallei (strain 668) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000570; BURPS668_3283 (Protoheme ferro-lyase) B2JGE7 HEMH_BURP8 hemH Bphy_2502 Burkholderia phymatum (strain DSM 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001043; 17167/STM815) (Protoheme ferro-lyase) B2SXB8 HEMH_BURPP hemH Bphyt_0733 Burkholderia phytofirmans (strain DSM 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001052; 17436/PsJN) (Protoheme ferro-lyase) Q63R43 HEMH_BURPS hemH BPSL2831 Burkholderia pseudomallei 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX571965; (Pseudomonas pseudomallei) (Protoheme ferro-lyase) Q39JD2 HEMH_BURS3 hemH Burkholderia sp. (strain 383) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000151; Bcep18194_A3835 (Burkholderia cepacia (strain ATCC (Protoheme ferro-lyase) 17760/NCIB 9086/R18194)) Q2SYZ9 HEMH_BURTA hemH BTH_I1303 Burkholderia thailandensis (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000086; E264/ATCC 700388/DSM 13276/ (Protoheme ferro-lyase) CIP 106301) A4JBR6 HEMH_BURVG hemH Bcep1808_0707 Burkholderia vietnamiensis (strain G4/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000614; LMG 22486) (Burkholderia cepacia (Protoheme ferro-lyase) (strain R1808)) Q145F5 HEMH_BURXL hemH Bxeno_A0496 Burkholderia xenovorans (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000270; Bxe_A3965 LB400) (Protoheme ferro-lyase) A7ZE60 HEMH_CAMC1 hemH Ccon26_12120 Campylobacter concisus (strain 13826) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000792; CCC13826_0053 (Protoheme ferro-lyase) A0RNU4 HEMH_CAMFF hemH CFF8240_0703 Campylobacter fetus subsp. fetus 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000487; (strain 82-40) (Protoheme ferro-lyase) A8FKS9 HEMH_CAMJ8 hemH C8J_0467 Campylobacter jejuni subsp. jejuni 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000814; serotype O:6 (strain 81116/NCTC (Protoheme ferro-lyase) 11828) A7H4N6 HEMH_CAMJD hemH JJD26997_1433 Campylobacter jejuni subsp. doylei 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000768; (strain ATCC BAA-1458/RM4099/ (Protoheme ferro-lyase) 269.97) Q9PI08 HEMH_CAMJE hemH Cj0503c Campylobacter jejuni 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AL111168; (Protoheme ferro-lyase) A1VYL5 HEMH_CAMJJ hemH CJJ81176_0531 Campylobacter jejuni subsp. jejuni 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000538; serotype O:23/36 (strain 81-176) (Protoheme ferro-lyase) Q5HVR0 HEMH_CAMJR hemH CJE0610 Campylobacter jejuni (strain RM1221) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000025; (Protoheme ferro-lyase) B8GW40 HEMH_CAUCN hemH CCNA_03878 Caulobacter crescentus (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001340; NA1000/CB15N) (Protoheme ferro-lyase) P57777 HEMH_CAUCR hemH CC_3762 Caulobacter crescentus (Caulobacter 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AF184071; AE005673; vibrioides) (Protoheme ferro-lyase) O42479 HEMH_CHICK FECH Gallus gallus (Chicken) 4.99.1.1 Ferrochelatase, mitochondrial (EC 4.99.1.1) (Heme U68033; synthase) (Protoheme ferro-lyase) Q5L6X6 HEMH_CHLAB hemH CAB136 Chlamydophila abortus 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CR848038; (Protoheme ferro-lyase) Q824K8 HEMH_CHLCV hemH CCA_00137 Chlamydophila caviae 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE015925; (Protoheme ferro-lyase) Q252Z7 HEMH_CHLFF hemH CF0869 Chlamydophila felis (strain Fe/C-56) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AP006861; (Protoheme ferro-lyase) Q9PJQ6 HEMH_CHLMU hemH TC_0772 Chlamydia muridarum 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE002160; (Protoheme ferro-lyase) Q9Z7V1 HEMH_CHLPN hemH CPn_0603 Chlamydia pneumoniae 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE001363; AE002161; BA000008; CP_0144 CpB0627 (Chlamydophila pneumoniae) (Protoheme ferro-lyase) AE009440; B0B858 HEMH_CHLT2 hemH CTL0746 Chlamydia trachomatis serovar L2 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AM884176; (strain 434/Bu/ATCC VR-902B) (Protoheme ferro-lyase) Q3KLL2 HEMH_CHLTA hemH CTA_0532 Chlamydia trachomatis serovar A 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000051; (strain HAR-13/ATCC VR-571B) (Protoheme ferro-lyase) B0BCC3 HEMH_CHLTB hemH CTLon_0741 Chlamydia trachomatis serovar L2b 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AM884177; (strain UCH-1/proctitis) (Protoheme ferro-lyase) O84492 HEMH_CHLTR hemH CT_485 Chlamydia trachomatis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE001273; (Protoheme ferro-lyase) Q7NV65 HEMH_CHRVO hemH CV_2480 Chromobacterium violaceum 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE016825; (Protoheme ferro-lyase) Q6NH66 HEMH_CORDI hemH DIP1280 Corynebacterium diphtheriae 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX248357; (Protoheme ferro-lyase) Q8FTB1 HEMH_COREF hemH CE1658 Corynebacterium efficiens 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BA000035; (Protoheme ferro-lyase) A4QEC1 HEMH_CORGB hemH cgR_1595 Corynebacterium glutamicum (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AP009044; R) (Protoheme ferro-lyase) Q8NQA1 HEMH_CORGL hemH Cgl1537 cg1734 Corynebacterium glutamicum 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BA000036; BX927152; (Brevibacterium flavum) (Protoheme ferro-lyase) B6J5R6 HEMH_COXB1 hemH CbuK_1987 Coxiella bumetii (strain CbuK_Q154) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001020; (Coxiella bumetii (strain Q154)) (Protoheme ferro-lyase) B6J386 HEMH_COXB2 hemH CbuG_1969 Coxiella bumetii (strain CbuG_Q212) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001019; (Coxiella bumetii (strain Q212)) (Protoheme ferro-lyase) A9KGX9 HEMH_COXBN hemH CBUD_2060 Coxiella bumetii (strain Dugway 5J108- 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000733; 111) (Protoheme ferro-lyase) Q83FA4 HEMH_COXBU hemH CBU_0042 Coxiella burnetii 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE016828; (Protoheme ferro-lyase) P42044 HEMH_CUCSA HEMH Cucumis sativus (Cucumber) 4.99.1.1 Ferrochelatase-2, chloroplastic (EC 4.99.1.1) D26106; (Ferrochelatase II) (Heme synthase 2) (Protoheme ferro-lyase 2) Q0KCJ6 HEMH_CUPNH hemH H16_A1134 Cupriavidus necator (strain ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AM260479; 17699/H16/DSM 428/Stanier 337) (Protoheme ferro-lyase) (Ralstonia eutropha) Q473L7 HEMH_CUPPJ hemH Reut_A1037 Cupriavidus pinatubonensis (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000090; JMP134/LMG 1197) (Alcaligenes (Protoheme ferro-lyase) eutrophus) (Ralstonia eutropha) B3R447 HEMH_CUPTR hemH RALTA_A1114 Cupriavidus taiwanensis (strain R1/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CU633749; LMG 19424) (Ralstonia taiwanensis (Protoheme ferro-lyase) (strain LMG 19424)) B8HK77 HEMH_CYAP4 hemH Cyan7425_4517 Cyanothece sp. (strain PCC 7425/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001344; ATCC 29141) (Protoheme ferro-lyase) B7KGB9 HEMH_CYAP7 hemH PCC7424_2167 Cyanothece sp. (strain PCC 7424) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001291; (Synechococcus sp. (strain ATCC (Protoheme ferro-lyase) 29155)) B7K399 HEMH_CYAP8 hemH PCC8801_0320 Cyanothece sp. (strain PCC 8801) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001287; (Synechococcus sp. (strain PCC 8801/ (Protoheme ferro-lyase) RF-1)) Q47HJ6 HEMH_DECAR hemH Daro_0929 Dechloromonas aromatica (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP900089; RCB) (Protoheme ferro-lyase) Q9RV98 HEMH_DEIRA hemH DR_1131 Deinococcus radiodurans 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE000513; (Protoheme ferro-lyase) Q6APB0 HEMH_DESPS hemH DP1085 Desulfotalea psychrophila 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CR522870; (Protoheme ferro-lyase) Q54IA8 HEMH_DICDI hemH DD8_G0288891 Dictyostelium discoideum (Slime mold) 4.99.1.1 Ferrochelatase, mitochondrial (EC 4.99.1.1) (Heme AAFI02000126; synthase) (Protoheme ferro-lyase) Q9V9S8 HEMH_DROME Fech CG2098 Drosophila melanogaster (Fruit fly) 4.99.1.1 Ferrochelatase, mitochondrial (EC 4.99.1.1) (Heme AF076220; AE014297; AE014297; synthase) (Protoheme ferro-lyase) AE014297; AY058251; BT001392; BT001878; A7ZIN5 HEMH_ECO24 hemH Escherichia coli O139:H28 (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000800; EcE24377A_0515 E24377A/ETEC) (Protoheme ferro-lyase) B7UKF5 HEMH_ECO27 hemH E2348_C_0410 Escherichia coli O127:H6 (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) FM180568; E2348/69/EPEC) (Protoheme ferro-lyase) B7MDZ7 HEMH_ECO45 hemH ECS88_0472 Escherichia coil O45:K1 (strain S88/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CU928161; ExPEC) (Protoheme ferro-lyase) B7L7A0 HEMH_ECO55 hemH EC55989_0488 Escherichia coli (strain 55989/EAEC) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CU928145; (Protoheme ferro-lyase) Q8XD39 HEMH_ECO57 hemH Z0592 ECs0528 Escherichia coli O157:H7 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE005174; BA000007; (Protoheme ferro-lyase) B5Z3Y6 HEMH_ECO5E hemH ECH74115_0568 Escherichia coli O157:H7 (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001164; EC4115/EHEC) (Protoheme ferro-lyase) B7NIF5 HEMH_ECO71 hemH ECIAI39_0196 Escherichia coli O7:K1 (strain IAI39/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CU928164; ExPEC) (Protoheme ferro-lyase) B7MQJ0 HEMH_ECO81 hemH ECED1_0498 Escherichia coli O81 (strain ED1a) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CU928162; (Protoheme ferro-lyase) B7M3W7 HEMH_ECO8A hemH ECIAI1_0478 Escherichia coli O8 (strain IAI1) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CU928160; (Protoheme ferro-lyase) C4ZUS9 HEMH_ECOBW hemH BWG_0356 Escherichia coli (strain K12/MC4100/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001396; BW2952) (Protoheme ferro-lyase) B1XFR2 HEMH_ECODH hemH ECDH10B_0431 Escherichia coli (strain K12/DH10B) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000948; (Protoheme ferro-lyase) A7ZXD3 HEMH_ECOHS hemH EcHS_A0552 Escherichia coli O9:H4 (strain HS) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000802; (Protoheme ferro-lyase) A1A8E1 HEMH_ECOK1 hemH Ecok1_04370 Escherichia coli O1:K1/APEC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000468; APECO1_1540 (Protoheme ferro-lyase) Q0TKG6 HEMH_ECOL5 hemH ECP_0536 Escherichia coli O6:K15:H31 (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000247; 536/UPEC) (Protoheme ferro-lyase) Q8FK83 HEMH_ECOL6 hemH c0595 Escherichia coli O6 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE014075; (Protoheme ferro-lyase) B1IZB9 HEMH_ECOLC hemH EcolC_3141 Escherichia coli (strain ATCC 8739/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000946; DSM 1576/Crooks) (Protoheme ferro-lyase) P23871 HEMH_ECOLI hemH popA visA b0475 Escherichia coli (strain K12) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) D90259; U82664; U00096; JW0464 (Protoheme ferro-lyase) AP009048; B7N928 HEMH_ECOLU hemH ECUMN_0514 Escherichia coli O17:K52:H18 (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CU928163; UMN026/ExPEC) (Protoheme ferro-lyase) B6I0C7 HEMH_ECOSE hemH ECSE_0500 Escherichia coli (strain SE11) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AP009240; (Protoheme ferro-lyase) B1LJN3 HEMH_ECOSM hemH EcSMS35_0520 Escherichia coli (strain SMS-3-5/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000970; SECEC) (Protoheme ferro-lyase) Q1RF60 HEMH_ECOUT hemH UTI89_C0503 Escherichia coli (strain UTI89/UPEC) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000243; (Protoheme ferro-lyase) C5BD17 HEMH_EDWI9 hemH NT01EI_1124 Edwardsiella ictaluri (strain 93-146) 499.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001600; (Protoheme ferro-lyase) A4W7F9 HEMH_ENT38 hemH Ent638_0955 Enterobacter sp. (strain 638) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000653; (Protoheme ferro-lyase) Q833G5 HEMH_ENTFA hemH EF_1989 Enterococcus faecalis (Streptococcus 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE016830; faecalis) (Protoheme ferro-lyase) A7MNM8 HEMH_ENTS8 hemH ESA_02788 Enterobacter sakazakii (strain ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000783; BAA-894) (Protoheme ferro-lyase) Q6D7Z4 HEMH_ERWCT hemH ECA1181 Erwinia carotovora subsp. atroseptica 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX950851; (Pectobacterium atrosepticum) (Protoheme ferro-lyase) B2VHX1 HEMH_ERWT9 hemH ETA_24640 Erwinia tasmaniensis (strain DSM 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CU468135; 17950/Et1/99) (Protoheme ferro-lyase) B7LV14 HEMH_ESCF3 hemH EFER_0525 Escherichia fergusonii (strain ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CU928158; 35469/DSM 13698/CDC 0568-73) (Protoheme ferro-lyase) Q0RH75 HEMH_FRAAA hemH FRAAL4516 Frankia alni (strain ACN14a) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CT573213; (Protoheme ferro-lyase) Q14H85 HEMH_FRAT1 hemH FTF1138 Francisella tularensis subsp. tularensis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AM286280; (strain FSC 198) (Protoheme ferro-lyase) A7NBJ3 HEMH_FRATF hemH FTA_0870 Francisella tularensis subsp. holarctica 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000803; (strain FTNF002-00/FTA) (Protoheme ferro-lyase) Q2A406 HEMH_FRATH hemH FTL_0821 Francisella tularensis subsp. holarctica 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AM233362; (strain LVS) (Protoheme ferro-lyase) B2SDE2 HEMH_FRATM hemH FTM_1306 Francisella tularensis subsp. 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000915; mediasiatica (strain FSC147) (Protoheme ferro-lyase) A0Q6Z1 HEMH_FRATN hemH FTN_1120 Francisella tularensis subsp. novicida 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000439; (strain U112) (Protoheme ferro-lyase) Q0BME1 HEMH_FRATO hemH FTH_0813 Francisella tularensis subsp. holarctica 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000437; (strain OSU18) (Protoheme ferro-lyase) Q5NFT3 HEMH_FRATT hemH FTT_1138 Francisella tularensis subsp. tularensis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AJ749949; (Protoheme ferro-lyase) A4IYH5 HEMH_FRATW hemH FTW_1172 Francisella tularensis subsp. tularensis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000608; (strain WY96-3418) (Protoheme ferro-lyase) B5EJ44 HEMH_GEOBB hemH Gbem_0039 Geobacter bemidjiensis (strain Bem/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001124; ATCC BAA-1014/DSM 16622) (Protoheme ferro-lyase) Q5L283 HEMH_GEOKA hemH GK0662 Geobacillus kaustophilus 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BA000043; (Protoheme ferro-lyase) Q39ZQ5 HEMH_GEOMG hemH Gmet_0019 Geobacter metallireducens (strain GS- 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000148; 15/ATCC 53774/DSM 7210) (Protoheme ferro-lyase) B9M326 HEMH_GEOSF hemH Geob_1076 Geobacter sp. (strain FRC-32) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001390; (Protoheme ferro-lyase) Q747F5 HEMH_GEOSL hemH GSU3312 Geobacter sulfurreducens 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE017180; (Protoheme ferro-lyase) C6E7U2 HEMH_GEOSM hemH GM21_0038 Geobacter sp. (strain M21) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001661; (Protoheme ferro-lyase) C5D6M6 HEMH_GEOSW hemH GWCH70_0650 Geobacillus sp. (strain WCH70) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001638; (Protoheme ferro-lyase) A4IKU8 HEMH_GEOTN hemH GTNG_0570 Geobacillus thermodenitrificans (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000557; NG80-2) (Protoheme ferro-lyase) A5GDG7 HEMH_GEOUR hemH Gura_0173 Geobacter uraniireducens (strain Rf4) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000698; (Geobacter uraniumreducens) (Protoheme ferro-lyase) Q7NMC7 HEMH_GLOVI hemH gll0839 Gloeobacter violaceus 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BA000045; (Protoheme ferro-lyase) Q5FSJ9 HEMH_GLUOX hemH GOX0874 Gluconobacter oxydans 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000009; (Gluconobacter suboxydans) (Protoheme ferro-lyase) Q4QLD6 HEMH_HAEI8 hemH NTHI1329 Haemophilus influenzae (strain 86- 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000057; 028NP) (Protoheme ferro-lyase) A5UCU6 HEMH_HAEIE hemH CGSHiEE_06240 Haemophilus influenzae (strain PittEE) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000671; (Protoheme ferro-lyase) P43868 HEMH_HAEIN hemH visA HI_1160 Haemophilus influenzae (strain ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) L42023; 51907/DSM 11121/KW20/Rd) (Protoheme ferro-lyase) C4K332 HEMH_HAMD5 hemH HDEF_0207 Hamiltonella defensa subsp. 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001277; Acyrthosiphon pisum (strain 5AT) (Protoheme ferro-lyase) Q17YG0 HEMH_HELAH hemH Hac_0490 Helicobacter acinonychis (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AM260522; Sheeba) (Protoheme ferro-lyase) Q7VHH1 HEMH_HELHP hemH HH_0996 Helicobacter hepaticus 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE017125; (Protoheme ferro-lyase) B6JMR9 HEMH_HELP2 hemH HPP12_1045 Helicobacter pylori (strain P12) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001217; (Protoheme ferro-lyase) B5Z875 HEMH_HELPG hemH HPG27_1021 Helicobacter pylori (strain G27) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001173; (Protoheme ferro-lyase) Q1CSI9 HEMH_HELPH hemH HPAG1_1016 Helicobacter pylori (strain HPAG1) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000241; (Protoheme ferro-lyase) Q9ZKD4 HEMH_HELPJ hemH jhp_1005 Helicobacter pylori J99 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE001439; (Campylobacter pylori J99) (Protoheme ferro-lyase) B2UUI4 HEMH_HELPS hemH HPSH_05535 Helicobacter pylori (strain Shi470) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001072; (Protoheme ferro-lyase) P56107 HEMH_HELPY hemH HP_0376 Helicobacter pylori (Campylobacter 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE000511; pylori) (Protoheme ferro-lyase) A9B546 HEMH_HERA2 hemH Haur_3547 Herpetosiphon aurantiacus (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000875; ATCC 23779/DSM 785) (Protoheme ferro-lyase) A4G8D5 HEMH_HERAR hemH HEAR2650 Herminiimonas arsenicoxydans 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CU207211; (Protoheme ferro-lyase) P42045 HEMH_HORVU HEMH Hordeum vulgare (Barley) 4.99.1.1 Ferrochelatase-2, chloroplastic (EC 4.99.1.1) D26105; AF020791; (Ferrochelatase II) (Heme synthase 2) (Protoheme ferro-lyase 2) P22830 HEMH_HUMAN FECH Homo sapiens (Human) 4.99.1.1 Ferrochelatase, mitochondrial (EC 4.99.1.1) (Heme D00726; AJ250235; BT019958; synthase) (Protoheme ferro-lyase) AK292937; CH471096; BC039841; L36178; AF495859; Q0BWA5 HEMH_HYPNA hemH HNE_3567 Hyphomonas neptunium (strain ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000158; 15444) (Protoheme ferro-lyase) Q5QVZ8 HEMH_IDILO hemH IL2347 Idiomarina loihiensis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE017340; (Protoheme ferro-lyase) A6T228 HEMH_JANMA hemH mma_2885 Janthinobacterium sp. (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000269; Marseille) (Minibacterium massiliensis) (Protoheme ferro-lyase) B5Y0N2 HEMH_KLEP3 hemH KPK_4223 Klebsiella pneumoniae (strain 342) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000964; (Protoheme ferro-lyase) Q036X3 HEMH_LACC3 hemH LSEI_1991 Lactobacillus casei (strain ATCC 334) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000423; (Protoheme ferro-lyase) B3W963 HEMH_LACCB hemH LCABL_21650 Lactobacillus casei (strain BL23) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) FM177140; (Protoheme ferro-lyase) Q9CFB4 HEMH_LACLA hemH LL1567 L0194 Lactococcus lactis subsp. lactis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE005176; (Streptococcus lactis) (Protoheme ferro-lyase) A2RJS3 HEMH_LACLM hemH llmg_0934 Lactococcus lactis subsp. cremoris 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AM406671; (strain MG1363) (Protoheme ferro-lyase) Q02Y14 HEMH_LACLS hemH LACR_1661 Lactococcus lactis subsp. cremoris 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000425; (strain SK11) (Protoheme ferro-lyase) Q88XC3 HEMH_LACPL hemH lp_1296 Lactobacillus plantarum 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AL935255; (Protoheme ferro-lyase) A5VLS3 HEMH_LACRD hemH Lreu_1554 Lactobacillus reuteri (strain DSM 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000705; 20016) (Protoheme ferro-lyase) B2G944 HEMH_LACRJ hemH LAR_1460 Lactobacillus reuteri (strain JCM 1112) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AP007281; (Protoheme ferro-lyase) Q5X7W3 HEMH_LEGPA hemH lpp0492 Legionella pneumophila (strain Paris) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CR628336; (Protoheme ferro-lyase) A5IHG9 HEMH_LEGPC hemH LPC_2918 Legionella pneumophila (strain Corby) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000675; (Protoheme ferro-lyase) Q5ZYE7 HEMH_LEGPH hemH lpg0425 Legionella pneumophila subsp. 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE017354; pneumophila (strain Philadelphia 1/ (Protoheme ferro-lyase) ATCC 33152/DSM 7513) Q5WZB4 HEMH_LEGPL hemH lpl0468 Legionella pneumophila (strain Lens) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CR628337; (Protoheme ferro-lyase) Q6AHF2 HEMH_LEIXX hemH Lxx01090 Leifsonia xyli subsp. xyli 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE016822; (Protoheme ferro-lyase) B0SF60 HEMH_LEPBA hemH LBF_1123 Leptospira biflexa serovar Patoc (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000777; Patoc 1/Ames) (Protoheme ferro-lyase) Q8GCV0 HEMH_LEPBI hemH Leptospira biflexa 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AY164270; (Protoheme ferro-lyase) Q04NU7 HEMH_LEPBJ hemH LBJ_4018 Leptospira borgpetersenii serovar 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000351; Hardjo-bovis (strain JB197) (Protoheme ferro-lyase) Q04X34 HEMH_LEPBL hemH LBL_4018 Leptospira borgpetersenii serovar 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000349; Hardjo-bovis (strain L550) (Protoheme ferro-lyase) B0SNJ9 HEMH_LEPBP hemH LEPBI_I1164 Leptospira biflexa serovar Patoc (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000786; Patoc 1/ATCC 23582/Paris) (Protoheme ferro-lyase) Q03Z41 HEMH_LEUMM hemH LEUM_0412 Leuconostoc mesenteroides subsp. 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000414; mesenteroides (strain ATCC 8293/ (Protoheme ferro-lyase) NCDO 523) Q929G2 HEMH_LISIN hemH lin2314 Listeria innocua 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AL596171; (Protoheme ferro-lyase) Q71XF4 HEMH_LISMF hemH LMOf2365_2244 Listeria monocytogenes serotype 4b 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE017262; (strain F2365) (Protoheme ferro-lyase) Q8Y565 HEMH_LISMO hemH lmo2211 Listeria monocytogenes 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AL591982; (Protoheme ferro-lyase) Q65SV7 HEMH_MANSM hemH MS1346 Mannheimia succiniciproducens (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE016827; MBEL55E) (Protoheme ferro-lyase) A1U0R2 HEMH_MARAV hemH Maqu_1496 Marinobacter aquaeolei (strain ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000514; 700491/DSM 11845/VT8) (Protoheme ferro-lyase) (Marinobacter hydrocarbonoclasticus (strain DSM 11845)) A6VXF8 HEMH_MARMS hemH Mmwyl1_2215 Marinomonas sp. (strain MWYL1) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000749; (Protoheme ferro-lyase) Q11ES4 HEMH_MESSB hemH Meso_2724 Mesorhizobium sp. (strain BNC1) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000390; (Protoheme ferro-lyase) Q607T4 HEMH_METCA hemH MCA1671 Methylococcus capsulatus 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE017282; (Protoheme ferro-lyase) Q1H397 HEMH_METFK hemH Mfla_0772 Methylobacillus flagellatus (strain KT/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000284; ATCC 51484/DSM 6875) (Protoheme ferro-lyase) A2SIR1 HEMH_METPP hemH Mpe_A2495 Methylibium petroleiphilum (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000555; PM1) (Protoheme ferro-lyase) B8EJ84 HEMH_METSB hemH Msil_3692 Methylocella silvestris (strain BL2/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001280; DSM 15510/NCIMB 13906) (Protoheme ferro-lyase) B0JRN7 HEMH_MICAN hemH MAE_09650 Microcystis aeruginosa (strain NIES- 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AP009552; 843) (Protoheme ferro-lyase) P22315 HEMH_MOUSE Fech Mus musculus (Mouse) 4.99.1.1 Ferrochelatase, mitochondrial (EC 4.99.1.1) (Heme M61215; M59288; synthase) (Protoheme ferro-lyase) A0QHT7 HEMH_MYCA1 hemH MAV_3293 Mycobacterium avium (strain 104) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000479; (Protoheme ferro-lyase) B1MC29 HEMH_MYCA9 hemH MAB_2721c Mycobacterium abscessus (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CU458896; ATCC 19977/DSM 44196) (Protoheme ferro-lyase) O07401 HEMH_MYCAV hemH Mycobacterium avium 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AF002133; (Protoheme ferro-lyase) P0A577 HEMH_MYCBO hemH hemZ Mb1521 Mycobacterium bovis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX248339; (Protoheme ferro-lyase) A1KIS5 HEMH_MYCBP hemH BCG_1547 Mycobacterium bovis (strain BCG/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AM408590; Pasteur 1173P2) (Protoheme ferro-lyase) C1AND1 HEMH_MYCBT hemH JTY_1522 Mycobacterium bovis (strain BCG/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AP010918; Tokyo 172/ATCC 35737/TMC 1019) (Protoheme ferro-lyase) A4T9I1 HEMH_MYCGI hemH Mflv_3656 Mycobacterium gilvum (strain PYR- 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000656; GCK) (Mycobacterium flavescens (Protoheme ferro-lyase) (strain ATCC 700033/PYR-GCK)) B8ZS79 HEMH_MYCLB hemH MLBr01805 Mycobacterium leprae (strain Br4923) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) FM211192; (Protoheme ferro-lyase) Q9CBM2 HEMH_MYCLE hemH hemZ ML1805 Mycobacterium leprae 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AL583923; (Protoheme ferro-lyase) B2HPD6 HEMH_MYCMM hemH MMAR_2291 Mycobacterium marinum (strain ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000854; BAA-535/M) (Protoheme ferro-lyase) Q740Y1 HEMH_MYCPA hemH MAP_1211 Mycobacterium paratuberculosis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE016958; (Protoheme ferro-lyase) A0QX29 HEMH_MYCS2 hemH MSMEG_3152 Mycobacterium smegmatis (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000480; ATCC 700084/mc(2)155) (Protoheme ferro-lyase) A3PZF2 HEMH_MYCSJ hemH Mjls_2495 Mycobacterium sp. (strain JLS) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000580; (Protoheme ferro-lyase) A1UFU2 HEMH_MYCSK hemH Mkms_2503 Mycobacterium sp. (strain KMS) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000518; (Protoheme ferro-lyase) Q1B968 HEMH_MYCSS hemH Mmcs_2458 Mycobacterium sp. (strain MCS) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000384; (Protoheme ferro-lyase) A5U2I9 HEMH_MYCTA hemH MRA_1495 Mycobacterium tuberculosis (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000611; ATCC 25177/H37Ra) (Protoheme ferro-lyase) P0A576 HEMH_MYCTU hemH hemZ Rv1485 Mycobacterium tuberculosis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX842576; AE000516; MT1532 MTCY277.06 (Protoheme ferro-lyase) A0PNU6 HEMH_MYCUA hemH MUL_1493 Mycobacterium ulcerans (strain Agy99) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000325; (Protoheme ferro-lyase) A1T8R1 HEMH_MYCVP hemH Mvan_2754 Mycobacterium vanbaalenii (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000511; DSM 7251/PYR-1) (Protoheme ferro-lyase) Q5F9U6 HEMH_NEIG1 hemH NGO0293 Neisseria gonorrhoeae (strain ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE004969; 700825/FA 1090) (Protoheme ferro-lyase) Q9JVA5 HEMH_NEIMA hemH NMA0927 Neisseria meningitidis serogroup A 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AL157959; (Protoheme ferro-lyase) Q9K097 HEMH_NEIMB hemH NMB0718 Neisseria meningitidis serogroup B 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE002098; (Protoheme ferro-lyase) A1KSY0 HEMH_NEIMF hemH NMC0669 Neisseria meningitidis serogroup C/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AM421808; serotype 2a (strain ATCC 700532/ (Protoheme ferro-lyase) FAM18) Q82UK8 HEMH_NITEU hemH NE1476 Nitrosomonas europaea 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AL954747; (Protoheme ferro-lyase) Q1QI02 HEMH_NITHX hemH Nham_3415 Nitrobacter hamburgensis (strain X14/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000319; DSM 10229) (Protoheme ferro-lyase) Q3JAW7 HEMH_NITOC hemH Noc_1545 Nitrosococcus oceani (strain ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000127; 19707/NCIMB 11848) (Protoheme ferro-lyase) A6Q2Y9 HEMH_NITSB hemH NIS_0734 Nitratiruptor sp. (strain SB155-2) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AP009178; (Protoheme ferro-lyase) Q5YU18 HEMH_NOCFA hemH NFA_34750 Nocardia farcinica 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AP006618; (Protoheme ferro-lyase) B2J9P0 HEMH_NOSP7 hemH Npun_F2510 Nostoc punctiforme (strain ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001037; 29133/PCC 73102) (Protoheme ferro-lyase) Q8YQR8 HEMH_NOSS1 hemH alr3751 Nostoc sp. (strain PCC 7120/UTEX 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BA000019; 2576) (Protoheme ferro-lyase) Q8ERX9 HEMH_OCEIH hemH OB1168 Oceanobacillus iheyensis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BA000028; (Protoheme ferro-lyase) A6X6W2 HEMH_OCHA4 hemH Oant_4266 Ochrobactrum anthropi (strain ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000759; 49188/DSM 6882/NCTC 12168) (Protoheme ferro-lyase) B1ZSW3 HEMH_OPITP hemH Oter_2470 Opitutus terrae (strain DSM 11246/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001032; PB90-1) (Protoheme ferro-lyase) A2Y3Q5 HEMH_ORYSI HEMH Osi_018948 Oryza sativa subsp. indica (Rice) 4.99.1.1 Ferrochelatase-2, chloroplastic (EC 4.99.1.1) AB007120; (Ferrochelatase II) (Heme synthase 2) (Protoheme ferro-lyase 2) Q3YA36 HEMH_PANTR FECH Pan troglodytes (Chimpanzee) 4.99.1.1 Ferrochelatase, mitochondrial (EC 4.99.1.1) (Heme DQ149645; synthase) (Protoheme ferro-lyase) Q6MAW8 HEMH_PARUW hemH pc1557 Protochlamydia amoebophila (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX908798; UWE25) (Protoheme ferro-lyase) P57874 HEMH_PASMU hemH PM0789 Pasteurella multocida 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE004439; (Protoheme ferro-lyase) A1ASJ7 HEMH_PELPD hemH Ppro_2715 Pelobacter propionicus (strain DSM 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000482; 2379) (Protoheme ferro-lyase) Q4FNS1 HEMH_PELUB hemH SAR11_0346 Pelagibacter ubique 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000084; (Protoheme ferro-lyase) B4RD10 HEMH_PHEZH hemH PHZ_c3533 Phenylobacterium zucineum (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000747; HLK1) (Protoheme ferro-lyase) Q7N0P6 HEMH_PHOLL hemH plu3835 Photorhabdus luminescens subsp. 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX571871; laumondii (Protoheme ferro-lyase) Q6LTE0 HEMH_PHOPR hemH PBPRA1025 Photobacterium profundum 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CR378666; (Photobacterium sp. (strain SS9)) (Protoheme ferro-lyase) Q12BZ9 HEMH_POLSJ hemH Bpro_2013 Polaromonas sp. (strain JS666/ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000316; BAA-500) (Protoheme ferro-lyase) B2RHB4 HEMH_PORG3 hemH PGN_0240 Porphyromonas gingivalis (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AP009380; ATCC 33277/DSM 20709/JCM (Protoheme ferro-lyase) 12257) Q7MXP4 HEMH_PORGI hemH PG_0127 Porphyromonas gingivalis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE015924; (Bacteroides gingivalis) (Protoheme ferro-lyase) P72183 HEMH_PROFF hemH Propionibacterium freudenreichii 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) D85417; U51164; subsp. freudenreichii (Protoheme ferro-lyase) A3PBP9 HEMH_PROM0 hemH P9301_05511 Prochlorococcus marinus (strain MIT 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000576; 9301) (Protoheme ferro-lyase) A2C0Y4 HEMH_PROM1 hemH NATL1_05821 Prochlorococcus marinus (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000553; NATL1A) (Protoheme ferro-lyase) A8G3P0 HEMH_PROM2 hemH P9215_06061 Prochlorococcus marinus (strain MIT 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000825; 9215) (Protoheme ferro-lyase) A2C7Q7 HEMH_PROM3 hemH P9303_07661 Prochlorococcus marinus (strain MIT 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000554; 9303) (Protoheme ferro-lyase) A9BEE9 HEMH_PROM4 hemH P9211_05281 Prochlorococcus marinus (strain MIT 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000878; 9211) (Protoheme ferro-lyase) A2BVI7 HEMH_PROM5 hemH P9515_05891 Prochlorococcus marinus (strain MIT 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000552; 9515) (Protoheme ferro-lyase) Q31C09 HEMH_PROM9 hemH PMT9312_0525 Prochlorococcus marinus (strain MIT 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000111; 9312) (Protoheme ferro-lyase) Q7VD58 HEMH_PROMA hemH Pro_0525 Prochlorococcus marinus 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE017126; (Protoheme ferro-lyase) B4F1Q1 HEMH_PROMH hemH PMI2183 Proteus mirabilis (strain HI4320) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AM942759; (Protoheme ferro-lyase) Q7V6C6 HEMH_PROMM hemH PMT_1240 Prochlorococcus marinus (strain MIT 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX548175; 9313) (Protoheme ferro-lyase) Q7V2F5 HEMH_PROMP hemH PMM0525 Prochlorococcus marinus subsp. 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX548174; pastoris (strain CCMP1986/MED4) (Protoheme ferro-lyase) A2BQ06 HEMH_PROMS hemH A9601_05811 Prochlorococcus marinus (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000551; AS9601) (Protoheme ferro-lyase) Q46GQ1 HEMH_PROMT hemH PMN2A_1857 Prochlorococcus marinus (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000095; NATL2A) (Protoheme ferro-lyase) Q48MT5 HEMH_PSE14 hemH PSPPH_1016 Pseudomonas syringae pv. 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000058; phaseolicola (strain 1448A/Race 6) (Protoheme ferro-lyase) Q15NQ1 HEMH_PSEA6 hemH Patl_3987 Pseudoalteromonas atlantica (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000388; T6c/BAA-1087) (Protoheme ferro-lyase) A6VC51 HEMH_PSEA7 hemH PSPA7_5304 Pseudomonas aeruginosa (strain PA7) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000744; (Protoheme ferro-lyase) B7V0K1 HEMH_PSEA8 hemH PLES_50411 Pseudomonas aeruginosa (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) FM209186; LESB58) (Protoheme ferro-lyase) Q02G19 HEMH_PSEAB hemH PA14_61580 Pseudomonas aeruginosa (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000438; UCBPP-PA14) (Protoheme ferro-lyase) Q9HVD7 HEMH_PSEAE hemH PA4655 Pseudomonas aeruginosa 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE004091; (Protoheme ferro-lyase) Q1IEW1 HEMH_PSEE4 hemH PSEEN0886 Pseudomonas entomophila (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CT573326; L48) (Protoheme ferro-lyase) Q4K6B3 HEMH_PSEF5 hemH PFL_5141 Pseudomonas fluorescens (strain Pf-5/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000076; ATCC BAA-477) (Protoheme ferro-lyase) P57778 HEMH_PSEFC hemH Pseudomonas fluorescens biotype C 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AF314196; (Protoheme ferro-lyase) C3KDN7 HEMH_PSEFS hemH PFLU_0756 Pseudomonas fluorescens (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AM181176; SBW25) (Protoheme ferro-lyase) A4XR78 HEMH_PSEMY hemH Pmen_1077 Pseudomonas mendocina (strain ymp) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000680; (Protoheme ferro-lyase) A5VYH6 HEMH_PSEP1 hemH Pput_0772 Pseudomonas putida (strain F1/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000712; ATCC 700007) (Protoheme ferro-lyase) Q3K6Y7 HEMH_PSEPF hemH Pfl01_4730 Pseudomonas fluorescens (strain Pf0- 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000094; 1) (Protoheme ferro-lyase) B0KNF5 HEMH_PSEPG hemH PputGB1_0785 Pseudomonas putida (strain GB-1) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000926; (Protoheme ferro-lyase) Q88PV4 HEMH_PSEPK hemH PP_0744 Pseudomonas putida (strain KT2440) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE015451; (Protoheme ferro-lyase) B1JER5 HEMH_PSEPW hemH PputW619_4445 Pseudomonas putida (strain W619) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000949; (Protoheme ferro-lyase) Q888A2 HEMH_PSESM hemH PSPTO_1128 Pseudomonas syringae pv. tomato 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE016853; (Protoheme ferro-lyase) Q4ZXU9 HEMH_PSEU2 hemH Psyr_0967 Pseudomonas syringae pv. syringae 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000075; (strain B728a) (Protoheme ferro-lyase) A4VKZ1 HEMH_PSEU5 hemH PST_1968 Pseudomonas stutzeri (strain A1501) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000304; (Protoheme ferro-lyase) Q4FUR3 HEMH_PSYA2 hemH Psyc_0376 Psychrobacter arcticus (strain DSM 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000082; 17307/273-4) (Protoheme ferro-lyase) Q1QDQ5 HEMH_PSYCK hemH Pcryo_0415 Psychrobacter cryohalolentis (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000323; K5) (Protoheme ferro-lyase) A5WH56 HEMH_PSYWF hemH PsycPRwf_2057 Psychrobacter sp. (strain PRwf-1) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000713; (Protoheme ferro-lyase) Q1LPN9 HEMH_RALME hemH Rmet_1001 Ralstonia metallidurans (strain CH34/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000352; ATCC 43123/DSM 2839) (Protoheme ferro-lyase) B2UBQ0 HEMH_RALPJ hemH Rpic_2877 Ralstonia pickettii (strain 12J) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001068; (Protoheme ferro-lyase) Q8XW32 HEMH_RALSO hemH RSc2643 Ralstonia solanacearum 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AL646052; RS04573 (Pseudomonas solanacearum) (Protoheme ferro-lyase) B3PZU8 HEMH_RHIE6 hemH Rhizobium etli (strain CIAT 652) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001074; RHECIAT_CH0003810 (Protoheme ferro-lyase) Q2K4C4 HEMH_RHIEC hemH RHE_CH03556 Rhizobium etli (strain CFN 42/ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000133; 51251) (Protoheme ferro-lyase) Q1MBW8 HEMH_RHIL3 hemH RL4076 Rhizobium leguminosarum bv. viciae 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AM236080; (strain 3841) (Protoheme ferro-lyase) Q98H61 HEMH_RHILO hemH mir3019 Rhizobium loti (Mesorhizobium loti) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BA000012; (Protoheme ferro-lyase) B5ZPG3 HEMH_RHILW hemH Rleg2_3304 Rhizobium leguminosarum bv. trifolii 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001191; (strain WSM2304) (Protoheme ferro-lyase) Q92M52 HEMH_RHIME hemH R02803 Rhizobium meliloti (Sinorhizobium 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AL591688; AF461430; SMc04019 meliloti) (Protoheme ferro-lyase) Q7UFZ7 HEMH_RHOBA hemH RB8233 Rhodopirellula baltica 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX294147; (Protoheme ferro-lyase) Q59735 HEMH_RHOCA hemH Rhodobacter capsulatus 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) U34391; (Rhodopseudomonas capsulata) (Protoheme ferro-lyase) Q2IRD5 HEMH_RHOP2 hemH RPB_4542 Rhodopseudomonas palustris (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000250; HaA2) (Protoheme ferro-lyase) Q07H58 HEMH_RHOP5 hemH RPE_4807 Rhodopseudomonas palustris (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000463; BisA53) (Protoheme ferro-lyase) Q6NBF3 HEMH_RHOPA hemH RPA0875 Rhodopseudomonas palustris 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX572595; (Protoheme ferro-lyase) Q13CU0 HEMH_RHOPS hemH RPD_0861 Rhodopseudomonas palustris (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000283; BisB5) (Protoheme ferro-lyase) B3QF66 HEMH_RHOPT hemH Rpal_0943 Rhodopseudomonas palustris (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001096; TIE-1) (Protoheme ferro-lyase) A8GYD7 HEMH_RICB8 hemH A1I_07945 Rickettsia bellii (strain OSU 85-389) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000849; (Protoheme ferro-lyase) Q1RGK5 HEMH_RICBR hemH RBE_1428 Rickettsia bellii (strain RML369-C) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000087; (Protoheme ferro-lyase) A8F0B2 HEMH_RICCK hemH A1E_05670 Rickettsia canadensis (strain McKiel) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000409; (Protoheme ferro-lyase) Q92FV4 HEMH_RICCN hemH RC1373 Rickettsia conorii 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE006914; (Protoheme ferro-lyase) Q4UJN9 HEMH_RICFE hemH RF_1399 Rickettsia felis (Rickettsia azadi) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000053; (Protoheme ferro-lyase) Q9ZC84 HEMH_RICPR hemH RP884 Rickettsia prowazekii 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AJ235273; (Protoheme ferro-lyase) Q68VM9 HEMH_RICTY hemH RT0876 Rickettsia typhi 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE017197; (Protoheme ferro-lyase) B5EXN2 HEMH_SALA4 hemH SeAg_B0535 Salmonella agona (strain SL483) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001138; (Protoheme ferro-lyase) A9MLY4 HEMH_SALAR hemH SARI_02445 Salmonella arizonae (strain ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000880; BAA-731/CDC346-86/RSK2980) (Protoheme ferro-lyase) Q57S74 HEMH_SALCH hemH SCH_0531 Salmonella choleraesuis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE017220; (Protoheme ferro-lyase) B5FLJ9 HEMH_SALDC hemH SeD_A0536 Salmonella dublin (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001144; CT_02021853) (Protoheme ferro-lyase) B5QU78 HEMH_SALEP hemH SEN0470 Salmonella enteritidis PT4 (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AM933172; P125109) (Protoheme ferro-lyase) B5R613 HEMH_SALG2 hemH SG0500 Salmonella gallinarum (strain 287/91/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AM933173; NCTC 13346) (Protoheme ferro-lyase) B4T9I3 HEMH_SALHS hemH SeHA_C0595 Salmonella heidelberg (strain SL476) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001120; (Protoheme ferro-lyase) B4SWY3 HEMH_SALNS hemH SNSL254_A0541 Salmonella newport (strain SL254) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001113; (Protoheme ferro-lyase) Q5PFJ1 HEMH_SALPA hemH SPA2233 Salmonella paratyphi A 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000026; (Protoheme ferro-lyase) A9MW82 HEMH_SALPB hemH SPAB_03079 Salmonella paratyphi B (strain ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000886; BAA-1250/SPB7) (Protoheme ferro-lyase) C0PVE1 HEMH_SALPC hemH SPC_0504 Salmonella paratyphi C (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000857; RKS4594) (Protoheme ferro-lyase) B5BD43 HEMH_SALPK hemH SSPA2076 Salmonella paratyphi A (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) FM200053; AKU_12601) (Protoheme ferro-lyase) B4TMG7 HEMH_SALSV hemH SeSA_A0551 Salmonella schwarzengrund (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001127; CVM19633) (Protoheme ferro-lyase) Q8Z8T2 HEMH_SALTI hemH STY0533 t2371 Salmonella typhi 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AL627267; AE014613; (Protoheme ferro-lyase) P37408 HEMH_SALTY hemH visA STM0489 Salmonella typhimurium 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE006468; L26246; (Protoheme ferro-lyase) O59786 HEMH_SCHPO SPCC320.09 Schizosaccharomyces pombe (strain 4.99.1.1 Ferrochelatase, mitochondrial (EC 4.99.1.1) (Heme CU329672; ATCC 38366/972) (Fission yeast) synthase) (Protoheme ferro-lyase) A8GAV7 HEMH_SERP5 hemH Spro_1143 Serratia proteamaculans (strain 568) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000826; (Protoheme ferro-lyase) Q12KR9 HEMH_SHEDO hemH Sden_2678 Shewanella denitrificans (strain OS217/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000302; ATCC BAA-1090/DSM 15013) (Protoheme ferro-lyase) B2U4S8 HEMH_SHIB3 hemH SbBS512_E0408 Shigella boydii serotype 18 (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001063; CDC 3083-94/BS512) (Protoheme ferro-lyase) Q325C1 HEMH_SHIBS hemH SBO_0375 Shigella boydii serotype 4 (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000036; Sb227) (Protoheme ferro-lyase) Q32J55 HEMH_SHIDS hemH SDY_0444 Shigella dysenteriae serotype 1 (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000034; Sd197) (Protoheme ferro-lyase) Q0T7B0 HEMH_SHIF8 hemH SFV_0448 Shigella flexneri serotype 5b (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000266; 8401) (Protoheme ferro-lyase) Q83SE5 HEMH_SHIFL hemH SF0420 S0427 Shigella flexneri 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE005674; AE014073; (Protoheme ferro-lyase) Q3Z4S4 HEMH_SHISS hemH SSON_0462 Shigella sonnei (strain Ss046) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000038; (Protoheme ferro-lyase) Q2NV56 HEMH_SODGM hemH SG0694 Sodalis glossinidius (strain morsitans) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AP008232; (Protoheme ferro-lyase) Q5HEU3 HEMH_STAAC hemH SACOL1888 Staphylococcus aureus (strain COL) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000046; (Protoheme ferro-lyase) P64124 HEMH_STAAM hemH SAV1833 Staphylococcus aureus (strain Mu50/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BA000017; ATCC 700699) (Protoheme ferro-lyase) P64125 HEMH_STAAN hemH SA1651 Staphylococcus aureus (strain N315) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BA000018; (Protoheme ferro-lyase) Q6GFM4 HEMH_STAAR hemH hemF SAR1924 Staphylococcus aureus (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX571856; MRSA252) (Protoheme ferro-lyase) Q6G8A3 HEMH_STAAS hemH SAS1754 Staphylococcus aureus (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX571857; MSSA476) (Protoheme ferro-lyase) P64126 HEMH_STAAW hemH MW1773 Staphylococcus aureus (strain MW2) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BA000033; (Protoheme ferro-lyase) Q5HNA5 HEMH_STAEQ hemH SERP1367 Staphylococcus epidermidis (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000029; ATCC 35984/RP62A) (Protoheme ferro-lyase) Q8CNS1 HEMH_STAES hemH SE_1512 Staphylococcus epidermidis (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE015929; ATCC 12228) (Protoheme ferro-lyase) Q49YM6 HEMH_STAS1 hemH SSP0966 Staphylococcus saprophyticus subsp. 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AP008934; saprophyticus (strain ATCC 15305/ (Protoheme ferro-lyase) DSM 20229) Q82KJ6 HEMH_STRAW hemH SAV_2407 Streptomyces avermitilis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BA000030; (Protoheme ferro-lyase) O50533 HEMH_STRCO hemH SCO5859 Streptomyces coelicolor 4.99.1.1 Probable ferrochelatase (EC 4.99.1.1) (Heme AL939125; SC9B10.26 synthase) (Protoheme ferro-lyase) B1VXN6 HEMH_STRGG hemH SGR_1673 Streptomyces griseus subsp. griseus 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AP009493; (strain JCM 4626/NBRC 13350) (Protoheme ferro-lyase) B4SNS0 HEMH_STRM5 hemH Smal_3991 Stenotrophomonas maltophilia (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001111; R551-3) (Protoheme ferro-lyase) Q8CWW4 HEMH_STRMU hemH hemZ SMU_2063 Streptococcus mutans 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE014133; (Protoheme ferro-lyase) Q04KS4 HEMH_STRP2 hemH SPD_0895 Streptococcus pneumoniae serotype 2 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000410: (strain D39/NCTC 7466) (Protoheme ferro-lyase) C1C6Y5 HEMH_STRP7 hemH SP70585_1048 Streptococcus pneumoniae (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000918; 70585) (Protoheme ferro-lyase) B1IBG8 HEMH_STRPI hemH SPH_1111 Streptococcus pneumoniae (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000936; Hungary19A-6) (Protoheme ferro-lyase) B8ZPG2 HEMH_STRPJ hemH SPN23F09340 Streptococcus pneumoniae (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) FM211187; ATCC 700669/Spain 23F-1) (Protoheme ferro-lyase) Q97R30 HEMH_STRPN hemH SP_1009 Streptococcus pneumoniae 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE005672; (Protoheme ferro-lyase) B2IPG3 HEMH_STRPS hemH SPCG_0985 Streptococcus pneumoniae (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001033; CGSP14) (Protoheme ferro-lyase) Q8DQ04 HEMH_STRR6 hemH spr0914 Streptococcus pneumoniae (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE007317; ATCC BAA-255/R6) (Protoheme ferro-lyase) C1CE03 HEMH_STRZJ hemH SPJ_0949 Streptococcus pneumoniae (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000919; JJA) (Protoheme ferro-lyase) C1CRD1 HEMH_STRZT hemH SPT_1062 Streptococcus pneumoniae (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000921; Taiwan19F-14) (Protoheme ferro-lyase) Q30S35 HEMH_SULDN hemH Suden_0918 Sulfurimonas denitrificans (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000153; ATCC 33889/DSM 1251) (Protoheme ferro-lyase) (Thiomicrospira denitrificans (strain ATCC 33889/DSM 1251)) B2V955 HEMH_SULSY hemH Sulfurihydrogenibium sp. (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001080; SYO3AOP1_0845 YO3AOP1) (Protoheme ferro-lyase) Q67T48 HEMH_SYMTH hemH STH160 Symbiobacterium thermophilum 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AP006840; (Protoheme ferro-lyase) Q31S00 HEMH_SYNE7 hemH Synechococcus elongatus (strain PCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000100; Synpcc7942_0137 7942) (Anacystis nidulans R2) (Protoheme ferro-lyase) Q2JVK5 HEMH_SYNJA hemH CYA_1034 Synechococcus sp. (strain JA-3-3Ab) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000239; (Cyanobacteria bacterium Yellowstone (Protoheme ferro-lyase) A-Prime) Q2JHZ4 HEMH_SYNJB hemH CYB_2857 Synechococcus sp. (strain JA-2-3B′a(2- 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000240; 13)) (Cyanobacteria bacterium (Protoheme ferro-lyase) Yellowstone B-Prime) B1XL79 HEMH_SYNP2 hemH Synechococcus sp. (strain ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000951; SYNPCC7002_A2589 27264/PCC 7002/PR-6) (Protoheme ferro-lyase) (Agmenellum quadruplicatum) Q5N2B2 HEMH_SYNP6 hemH syc1368_c Synechococcus sp. (strain ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AP008231; 27144/PCC 6301/SAUG 1402/1) (Protoheme ferro-lyase) (Anacystis nidulans) A5GJF5 HEMH_SYNPW hemH Synechococcus sp. (strain WH7803) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CT971583; SynWH7803_0644 (Protoheme ferro-lyase) Q7U5G0 HEMH_SYNPX hemH SYNW1747 Synechococcus sp. (strain WH8102) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX569693; (Protoheme ferro-lyase) A5GS98 HEMH_SYNR3 hemH Synechococcus sp. (strain RCC307) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CT978603; SynRCC307_0854 (Protoheme ferro-lyase) Q0I8L9 HEMH_SYNS3 hemH sync_2000 Synechococcus sp. (strain CC9311) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000435; (Protoheme ferro-lyase) Q3ALP2 HEMH_SYNSC hemH Syncc9605_0716 Synechococcus sp. (strain CC9605) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000110; (Protoheme ferro-lyase) P54225 HEMH_SYNY3 hemH slr0839 Synechocystis sp. (strain ATCC 27184/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BA000022; PCC 6803/N-1) (Protoheme ferro-lyase) C5BIF4 HEMH_TERTT hemH TERTU_4338 Teredinibacter tumerae (strain ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001614; 39867/T7901) (Protoheme ferro-lyase) Q9HLB8 HEMH_THEAC hemH Ta0311 Thermoplasma acidophilum (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AL445063; ATCC 25905/DSM 1728/JCM 9062/ (Protoheme ferro-lyase) NBRC 15155/AMRC-C165) Q8DGU6 HEMH_THEEB hemH tlr2216 Thermosynechococcus elongatus 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BA000039; (strain BP-1) (Protoheme ferro-lyase) Q72L32 HEMH_THET2 hemH TT_C0231 Thermus thermophilus (strain HB27/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE017221; ATCC BAA-163/DSM 7039) (Protoheme ferro-lyase) Q978U9 HEMH_THEVO hemH TV1316 Thermoplasma volcanium (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BA000011; TVG1357926 ATCC 51530/DSM 4299/IFO 15438/ (Protoheme ferro-lyase) JCM 9571/GSS1) Q31EG9 HEMH_THICR hemH Tcr_1864 Thiomicrospira crunogena (strain XCL- 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000109; 2) (Protoheme ferro-lyase) Q3SHA3 HEMH_THIDA hemH Tbd_2033 Thiobacillus denitrificans (strain ATCC 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000116; 25259) (Protoheme ferro-lyase) B8GU82 HEMH_THISH hemH Tgr7_0266 Thioalkalivibrio sp. (strain HL-EbGR7) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001339; (Protoheme ferro-lyase) Q10WR6 HEMH_TRIEI hemH Tery_4313 Trichodesmium erythraeum (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000393; IMS101) (Protoheme ferro-lyase) Q83H94 HEMH_TROW8 hemH TW747 Tropheryma whipplei (strain TW08/27) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX251412; (Whipple's bacillus) (Protoheme ferro-lyase) Q83FJ2 HEMH_TROWT hemH TWT_733 Tropheryma whipplei (strain Twist) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE014184; (Whipple's bacillus) (Protoheme ferro-lyase) Q9KTB6 HEMH_VIBCH hemH VC_0987 Vibrio cholerae 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE003852; (Protoheme ferro-lyase) Q5E6Q7 HEMH_VIBF1 hemH VF_0794 Vibrio fischeri (strain ATCC 700601/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000020; ES114) (Protoheme ferro-lyase) B5FBZ6 HEMH_VIBFM hemH VFMJ11_0831 Vibrio fischeri (strain MJ11) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001139; (Protoheme ferro-lyase) Q87RH3 HEMH_VIBPA hemH VP0823 Vibrio parahaemolyticus 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BA000031; (Protoheme ferro-lyase) B7VII4 HEMH_VIBSL hemH VS_2267 Vibrio splendidus (strain LGP32) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) FM954972; (Vibrio splendidus (strain Mel32)) (Protoheme ferro-lyase) Q8DFM2 HEMH_VIBVU hemH VV1_0187 Vibrio vulnificus 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE016795; (Protoheme ferro-lyase) Q7MMR4 HEMH_VIBVY hemH VV1003 Vibrio vulnificus (strain YJ016) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BA000037; (Protoheme ferro-lyase) Q8D226 HEMH_WIGBR hemH WIGBR5290 Wigglesworthia glossinidia brevipalpis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BA000021; (Protoheme ferro-lyase) Q73FY6 HEMH_WOLPM hemH WD_1186 Wolbachia pipientis wMel 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE017196; (Protoheme ferro-lyase) B3CLU1 HEMH_WOLPP hemH WP0751 Wolbachia pipientis subsp. Culex 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AM999887; pipiens (strain wPip) (Protoheme ferro-lyase) Q7M7P9 HEMH_WOLSU hemH WS2157 Wolinella succinogenes 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX571662; (Protoheme ferro-lyase) Q5GRR7 HEMH_WOLTR hemH Wbm0719 Wolbachia sp. subsp. Brugia malayi 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE017321; (strain TRS) (Protoheme ferro-lyase) C0R4L0 HEMH_WOLWR hemH WRi_011580 Wolbachia sp. subsp. Drosophila 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001391; simulans (strain wRi) (Protoheme ferro-lyase) Q8PEX0 HEMH_XANAC hemH XAC4220 Xanthomonas axonopodis pv. citri 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE008923; (Citrus canker) (Protoheme ferro-lyase) Q8P3H6 HEMH_XANCP hemH XCC4095 Xanthomonas campestris pv. 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE008922; campestris (Protoheme ferro-lyase) O57478 HEMH_XENLA fech Xenopus laevis (African clawed frog) 4.99.1.1 Ferrochelatase, mitochondrial (EC 4.99.1.1) (Heme AF036617; synthase) (Protoheme ferro-lyase) B2I7H7 HEMH_XYLF2 hemH XfasM23_1664 Xylella fastidiosa (strain M23) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001011; (Protoheme ferro-lyase) Q9PFU1 HEMH_XYLFA hemH XF_0566 Xylella fastidiosa 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE003849; (Protoheme ferro-lyase) B0U456 HEMH_XYLFM hemH Xfasm12_1736 Xylella fastidiosa (strain M12) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000941; (Protoheme ferro-lyase) Q87B82 HEMH_XYLFT hemH PD_1576 Xylella fastidiosa (strain Temecula1/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AE009442; ATCC 700964) (Protoheme ferro-lyase) P16622 HEMH_YEAST HEM15 YOR176W Saccharomyces cerevisiae (strain 4.99.1.1 Ferrochelatase, mitochondrial (EC 4.99.1.1) (Heme J05395; X54514; Z75084; ATCC 204508/S288c) (Baker's yeast) synthase) (Protoheme ferro-lyase) BK006948; A1JNA4 HEMH_YERE8 hemH YE3088 Yersinia enterocolitica serotype O:8/ 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AM286415; biotype 1B (strain 8081) (Protoheme ferro-lyase) P43413 HEMH_YEREN hemH Yersinia enterocolitica 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) Z47767; U46859; (Protoheme ferro-lyase) A7FL85 HEMH_YERP3 hemH Yersinia pseudotuberculosis serotype 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000720; YpsIP31758_3053 O:1b (strain IP 31758) (Protoheme ferro-lyase) Q1C4Q0 HEMH_YERPA hemH YPA_2610 Yersinia pestis bv. Antiqua (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000308; Antiqua) (Protoheme ferro-lyase) B2K6Z7 HEMH_YERPB hemH YPTS_1040 Yersinia pseudotuberculosis serotype 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP001048; IB (strain PB1/+) (Protoheme ferro-lyase) Q8ZC98 HEMH_YERPE hemH YPO3117 y1066 Yersinia pestis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AL590842; AE009952; AE017042; YP_0813 (Protoheme ferro-lyase) A9R0Q8 HEMH_YERPG hemH YpAngola_A2895 Yersinia pestis bv. Antiqua (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000901; Angola) (Protoheme ferro-lyase) Q1CL26 HEMH_YERPN hemH YPN_0972 Yersinia pestis bv. Antiqua (strain 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000305; ACNQ01000008; YP516_1054 Nepal516) (Protoheme ferro-lyase) A4TPA3 HEMH_YERPP hemH YPDSF_2753 Yersinia pestis (strain Pestoides F) 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000668; (Protoheme ferro-lyase) Q05338 HEMH_YERPS hemH YPTB0997 Yersinia pseudotuberculosis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) BX936398; AF461770; (Protoheme ferro-lyase) B1JHN0 HEMH_YERPY hemH YPK_3193 Yersinia pseudotuberculosis serotype 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) CP000950; O:3 (strain YPIII) (Protoheme ferro-lyase) P57779 HEMH_ZYMMO hemH ZMO0303 Zymomonas mobilis 4.99.1.1 Ferrochelatase (EC 4.99.1.1) (Heme synthase) AF212041; AE008692; (Protoheme ferro-lyase)

Suitable ferrochelatases of the present invention can also be identified by homology or sequence identity to the ferrochelatases disclosed in Table 3. Suitable ferrochelatases include those having an amino acid or nucleotide sequence identity of at least about 25 percent, more preferably at least about 30 to 40 percent, more preferably at least 50 to 60 percent, more preferably at least about 70 to 80 percent, most preferably at least about 85 to 95 percent as compared to a characterized ferrochelatase sequence of Table 3.

Mutant or modified ferrochelatase enzymes that retain their enzymatic activity (i.e., capable of catalyzing the insertion of ferrous iron into protoporphyrin IX to form protoheme (EC 4.99.1.1)) are also suitable for use in the present invention. Modifications or mutations to known ferrochelatase nucleotide sequences, such as nucleotide deletions, insertions, or substitutions that do not alter the coding sequence of the active region of the ferrochelatase enzyme or do not alter the activity of the encoded ferrochelatase enzyme are suitable for use in the methods of the present invention. For example, eukaryotic ferrochelatases possess an amino-terminus signal sequence that is lacking in prokaryote ferrochelatases (Dailey et al., “Ferrochelatase at the Millennium: Structures, Mechanisms, and [2Fe-2S] Clusters,” Cell. Mol. Life. Sci. 57:1909-1926 (2000) which is hereby incorporated by reference in its entirety). Therefore, recombinant eukaryotic ferrochelatase sequences containing modifications or deletions to this portion of the encoded protein that do not alter enzyme activity are suitable for use in the present invention. Likewise, eukaryotic ferrochelatases also possess a carboxyl-terminal extension sequence that is lacking in a majority of prokaryotic ferrochelatases (Dailey et al., “Ferrochelatase at the Millennium: Structures, Mechanisms, and [2Fe-2S] Clusters,” Cell. Mol. Life. Sci. 57:1909-1926 (2000), which is hereby incorporated by reference in its entirety). Recombinant eukaryotic ferrochelatase sequences containing modifications or deletions to this extension region are also suitable for use in the methods of the present invention. Similarly, recombinant ferrochelatase sequences containing single-base mutations that do not alter the conserved amino acid residues of the ferrochelatase protein acids (Dailey et al., “Ferrochelatase at the Millennium: Structures, Mechanisms, and [2Fe-2S] Clusters,” Cell. Mol. Life. Sci. 57:1909-1926 (2000), which is hereby incorporated by reference in its entirety) or the enzyme active site are also suitable for use in the present invention.

In accordance with this aspect of the invention, co-expression of the recombinant heme-binding protein and ferrochelatase protein, or polypeptide thereof, is carried out in the presence of one or more heme precursors, Suitable heme precursors include, without limitation, δ-amino levulinic acid, succinyl CoA, glycine, glutamate, glutamate-1 semialdehyde, porphobilinogen, hydroxymethylbilane, and protoporphyrin.

Co-expression of the recombinant heme-binding protein and ferrochelatase protein or polypeptide can be carried out in any one of the commonly known systems that are available in the art for heterologous protein expression, including, without limitation, eukaryotic and prokaryotic expression systems, and cell-free translation systems as described herein.

Techniques and protocols for manipulation of nucleic acids, including, for example, the preparation of nucleic acid constructs, mutagenesis, sequencing, introduction of DNA into cells and gene expression, and analysis of proteins, are described in detail in Current Protocols in Molecular Biology, Ausubel et al. eds., (1992), which is hereby incorporated by reference in its entirety.

Typically, a nucleic acid molecule encoding all or part of a protein of interest, i.e., a heme-binding protein, is obtained using methods such as those described herein. The protein-encoding nucleic acid sequence is cloned into an expression vector that is suitable for the particular host cell of interest using standard recombinant DNA procedures. Suitable expression vectors include those which contain replicon and control sequences that are derived from species compatible with the host cell.

Expression vectors include (among other elements) regulatory sequences (e.g., promoters) that can be operably linked to the desired protein-encoding nucleic acid molecule to cause the expression of such nucleic acid molecule in the host cell. Together, the regulatory sequences and the protein-encoding nucleic acid sequence are an expression construct. Expression vectors may also include an origin of replication, marker genes that provide phenotypic selection in transformed cells, one or more other promoters, and a polylinker region containing several restriction sites for insertion of heterologous nucleic acid sequences.

Expression vectors useful for expression of heterologous protein(s) in a multitude of host cells are well known in the art (Sambrook and Russell, Molecular Cloning: a Laboratory Manual 3rd ed. (2001), which is hereby incorporated by reference in its entirety), and some specific examples are provided herein. The host cell is transfected with (or infected with a virus containing) the expression vector using any method suitable for the particular host cell. Such transfection methods are also well known in the art and non-limiting exemplary methods are described herein. The transfected (also called, transformed) host cell is capable of expressing the protein encoded by the corresponding nucleic acid sequence in the expression construct. Transient or stable transfection of the host cell with one or more expression vectors is contemplated by the present disclosure.

In one embodiment of the present invention, the nucleotide sequence encoding the desired heme-binding protein is inserted into one expression vector and the nucleotide sequence encoding the ferrochelatase is inserted into a second expression vector. In this embodiment, the two expression vectors are co-transfected into an appropriate host cell for transcription and translation. In another embodiment of the invention, both nucleotide sequences are inserted into one expression vector and the single expression vector encodes both the recombinant heme-binding protein and the recombinant ferrochelatase.

Many different types of cells may be used to express heterologous proteins, such as bacterial, archaeal, yeast, fungal, insect, vertebrate (such as mammalian cells), and plant cells, including primary cells and immortal cell lines. Numerous representatives of each cell type are commonly used and are available from a wide variety of commercial sources, including, for example, the American Tissue Culture Collection (ATCC). Further details of some specific embodiments are discussed below.

Prokaryotes, such as bacteria, may be used as host cells. Prokaryotic expression systems are advantageous, at least, because of culture affordability, ease of genetic manipulation, and high yields of desired product(s). As described herein, E. coli BL21 (DE3) is a suitable prokaryotic host cell. Other suitable E. coli host cells include, without limitation, E. coli K12 strain 94 (ATCC No. 31,446), coli strain W3 110 (ATCC No, 27,325), E. coli X1776 (ATCC No, 31,537), E. coli B, and many other strains, such as HB101, JM101, NM522, NM538, NM539, B1-21, B1-21 (DE3) pLysS, Origami B, OmpT-defective CD41, CD43 (DE3), and phosphatidylenthanolamine (PE)-deficient AD93. Similarly, other species and genera of prokaryotes including Pseudomonas aeruginosa, Salmonella gastroenteritis (typhimirium), S. typhi, S. enteriditis, Shigella flexneri, S. sonnie, S. dysenteriae, Neisseria gonorrhoeae, N. meningitides, Haemophilus influenzae, H. pleuropneumoniae, Pasteurella haemolytica, P. multilocida, Legionella pneumophila, Treponema pallidum, T. denticola, T. orales, Borrelia burgdorferi, Borrelia spp., Leptospira interrogans, Klebsiella pneumoniae, Proteus vulgaris, P. morganii, P. mirabilis, Rickettsia prowazeki, R. typhi, R. richettsii, Porphyromonas (Bacteriodes) gingivalis, Chlamydia psittaci, C. pneumoniae, C. trachomatis, Campylobacter jejuni, C. intermedis, C. fetus, Helicobacter pylori, Francisella tularenisis, Vibrio cholerae, Vibrio parahaemolyticus, Bordetella pertussis, Burkholderie pseudomallei, Brucella abortus, B. susi, B. melitensis, B. canis, Spirillum minus, Pseudomonas mallei, Aeromonas hydrophila, A. salmonicida, Lactococcus lactis, and Yersinia pestis, may all be used as prokaryotic expression hosts.

Prokaryotic host cells or other host cells with rigid cell walls may be transformed using any method known in the art, including, for example, calcium phosphate precipitation, or electroporation. Representative prokaryote transformation techniques are described in Hanahan et al., “Plasmid Transformation of Escherichia coli and Other Bacteria,”Meth. Enzymol., 204:63-113 (1991), which is hereby incorporated by reference in its entirety.

Vectors typically used for transformation of E. coli include, without limitation, pBR322, pUC18, pUC19, pUC118, pUC119, Bluescript M13 and derivatives thereof. Numerous such plasmids are commercially available and are well known in the art.

Different genetic signals and processing events control many levels of gene expression (e.g., DNA transcription and messenger RNA (“mRNA”) translation). Transcription of DNA is dependent upon the presence of a promoter, which is a DNA sequence that directs the binding of RNA polymerase, and thereby promotes mRNA synthesis. Promoters vary in their strength (i.e., their ability to promote transcription). For the purposes of expressing a cloned gene, it is desirable to use strong promoters to obtain a high level of transcription and, hence, expression. Therefore, depending upon the host system utilized, any one of a number of suitable promoters may also be incorporated into the expression vector carrying the nucleic acid molecule(s) of the present invention. For instance, when using E. coli, its bacteriophages, or plasmids, promoters such as the T7 phage promoter, lac promoter, trp promoter, recA promoter, ribosomal RNA promoter, the P_(R) and P_(L) promoters of coliphage lambda and others, including but not limited, to lacUV5, ompF, lpp, and the like, may be used to direct high levels of transcription of adjacent DNA segments. Additionally, a hybrid trp-lacUV5 (tac) promoter or other E. coli promoters produced by recombinant DNA or other synthetic DNA techniques may be used to provide for transcription of the inserted gene.

Translation of mRNA in prokaryotes depends upon the presence of the proper prokaryotic signals, which differ from those of eukaryotes. Efficient translation of mRNA in prokaryotes requires a ribosome binding site called the Shine-Dalgarno (“SD”) sequence on the mRNA. This sequence is a short nucleotide sequence of mRNA that is located before the start codon, usually AUG, which encodes the amino-terminal methionine of the protein. The SD sequences are complementary to the 3′-end of the 16S rRNA (ribosomal RNA) and probably promote binding of mRNA to ribosomes by duplexing with the rRNA to allow correct positioning of the ribosome. For a review on maximizing gene expression, see Roberts and Lauer, Methods in Enzymology, 68:473 (1979), which is hereby incorporated by reference in its entirety.

Recombinant heme-binding proteins can also be produced in archaeal expression systems. Species that are suitable for serving as hosts include, without limitation, Methanosarcina acetivorans and Sulfolobus solfataricus (see e.g., Albers et al., “Production of Recombinant and Tagged Proteins in the Hyerthermophilic Archaeon Sulfolobus solfataricus,” Appl. Environ. Microbial. 72(1):102-11 (2006) and Jonuscheit et al., “A Reporter Gene System for the Hyperthermophilic Archaean Sulfolobus solfataricus based on a Selectable and Integrative Shuttle Vector,”Mol. Microbiol. 48(5):1241-52 (2003), which is hereby incorporated by reference in its entirety).

Fungal protein expression systems can also be utilized in the methods of the present invention to efficiently produce a functional recombinant heme-binding protein. Fungal species are considered safer than animal cells because they pose little risk of contamination by viruses, prions, or endotoxins. Additionally, fungal systems are more efficient and economical than mammalian expression systems. There are several fungal species which have been used as hosts for the expression of recombinant proteins, including, without limitation, yeast (e.g., Pichia pastoris, Kluyveromyces lactis, and Saccharomyces cerevisiae), soil fungus (e.g., Trichoderma reesei), and black mould fungus (e.g., Aspergillus niger).

Yeast strains and yeast-derived vectors are used commonly for the expression of heterologous proteins. For instance, Pichia pastoris expression systems, may be used to co-express a recombinant heme-binding protein of interest with ferrochelatase (see e.g., Ballew N., “Revolutionizing Protein Production in Fungi,” Innovations in Pharmaceutical Technology 70-76 (June 2004), which is hereby incorporated by reference in its entirety). Such systems include suitable Pichia pastoris strains, vectors, reagents, transformants, sequencing primers, and media. Available strains include KM71H (a prototrophic strain), SMD1168H (a prototrophic strain), and SMD1168 (a pep4 mutant strain) (Invitrogen).

Saccharomyces cerevisiae is also commonly used in heterologous expression systems. The plasmid YRp7 is commonly used as an expression vector in Saccharomyces (Stinchcomb et al., “Isolation and Characterization of a Yeast Chromosomal Replicator,” Nature 282:39-43 (1979); Kingsman et al., “Replication in Saccharomyces Cerevisiae of Plasmid pBR313 Carrying DNA from the Yeast trp1 Region,” Gene 7:141-152 (1979); and Tschemper et al., “Sequence of a Yeast DNA Fragment Containing a Chromosomal Replicator and the TRP1 Gene,” Gene 10:157-166 (1980), which are hereby incorporated by reference in their entirety). This plasmid contains the trp1 gene that provides a selection marker for a mutant strain of yeast lacking the ability to grow in tryptophan, such as strains ATCC No. 44,076 and PEP4-1 (Jones, E. W., “Proteinase Mutants of Saccharomyces cerevisiae,” Genetics 85:23-33 (1977), which is hereby incorporated by reference in its entirety). The presence of the trp1 lesion as a characteristic of the yeast host cell genome then provides an effective environment for detecting transformation by growth in the absence of tryptophan.

Yeast host cells can be transformed using the polyethylene glycol method, as described by Hinnen (Hinnen et al., “Transformation of Yeast,” Proc. Natl. Acad. Sci. U.S.A. 75:1929-1933 (1978), which is hereby incorporated by reference in its entirety). Additional yeast transformation protocols are set forth in Gietz et al., “Improved Method for High Efficiency Transformation of Intact Yeast Cells,” Nucl. Acids Res. 20(6):1425 (1992)) and Reeves et al. “A Yeast Intron as a Translation Terminator in a Plasmid Shuttle Vector,” Yeast Res. 4(6):573-597 (2004), which are hereby incorporated by reference in their entirety.

Suitable promoting sequences in yeast vectors include the promoters for 3-phosphoglycerate kinase (Hitzeman et al., “Isolation and Characterization of the Yeast 3-Phosphoglycerokinase Gene (PGK) by an Immunological Screening Technique,” J. Biol. Chem. 255:12073-12080 (1980), which is hereby incorporated by reference in its entirety) or other glycolytic enzymes (Hess et al., “Cooperation of Glycolytic Enzymes,” J. Adv. Enzyme Reg. 7:149-167 (1969) and Holland et al., “Isolation and Identification of Yeast Messenger Ribonucleic Acids Coding for Enolase, Glyceraldehyde-3-Phosphate Dehydrogenase, and Phosphoglycerate Kinase,” Biochem. 17:4900-4907 (1978), which are hereby incorporated by referenced in their entirety), such as enolase, glyceraldehyde-3-phosphate dehydrogenase, hexokinase, pyruvate decarboxylase, phosphofructokinase, glucose-6-phosphate isomerase, 3-phosphoglycerate mutase, pyruvate kinase, triosephosphate isomerase, phosphoglucose isomerase, and glucokinase. In the construction of suitable expression vectors, the termination sequences associated with these genes are also ligated into the expression vector 3′ of the sequence desired to be expressed to provide polyadenylation of the mRNA and termination. Other promoters that have the additional advantage of transcription controlled by growth conditions are the promoter region for alcohol dehydrogenase 2, isocytochrome C, acid phosphatase, degradative enzymes associated with nitrogen metabolism, and the aforementioned glyceraldehyde-3-phosphate dehydrogenase, and enzymes responsible for maltose and galactose utilization. Any plasmid vector containing yeast-compatible promoter, origin of replication and termination sequences is suitable.

Another representative eukaryotic expression system involves the recombinant baculoviruses, Autographa californica nuclear polyhedrosis virus (AcNPV; Summers and Smith, A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures Texas Agriculture Experiment Station. Bulletin No. 1555 (1987) and Luckow et al., “Trends in the Development of Baculovirus Expression Vectors,” Biotechnol. 6:47-55 (1987), which are hereby incorporated by referenced in their entirety) and Spodoptera frugiperda. Baculoviruses do not infect humans and can therefore be safely handled in large quantities.

A baculovirus expression vector is prepared as previously described using standard molecular biology techniques. The vector may comprise the polyhedron gene promoter region of a baculovirus, the baculovirus flanking sequences necessary for proper crossover during recombination (the flanking sequences comprise about 200-300 base pairs adjacent to the promoter sequence) and a bacterial origin of replication which permits the construct to replicate in bacteria. In particular examples, the vector is constructed so that a heme-binding protein and ferrochelatase nucleic acid sequences are operably linked to the polyhedron gene promoter (collectively, the “expression construct”) and the expression construct is flanked by the above-described baculovirus flanking sequences. Appropriate transfer vectors compatible with insect host cells are known in the art and include, without limitation, pVL1392, pVL1393, pAcGP67 and pAcSecG2T, which incorporate asecretory signal fused to the desired protein, and pAcGHLT and pAcHLT, which contain GST and 6×His tags (BD Biosciences, Franklin Lakes, N.J.).

Insect host cells (e.g., Spodoptera frugiperda cells) are infected with a recombinant baculovirus and cultured under conditions allowing expression of the baculovirus-encoded heme-binding proteins and ferrochelatase protein or polypeptide. When using insect cells, suitable baculovirus promoters include late promoters, such as 39K protein promoter or basic protein promoter, and very late promoters, such as the p10 and polyhedron promoters. In some cases it may be desirable to use transfer vectors containing multiple baculoviral promoters. The expressed heme-binding protein may be extracted from the insect cells using methods known in the art.

Mammalian host cells may also be used for heterologous expression of a heme-binding protein and ferrochelatase protein or polypeptide. Examples of suitable mammalian cell lines include, without limitation, monkey kidney CVI line transformed by SV40 (COS-7, ATCC CRL 1651); human embryonic kidney line 293S (Graham et al., “Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5,” J. Gen. Virol. 36:59-74 (1977), which is hereby incorporated by reference in its entirety); baby hamster kidney cells (BHK, ATCC CCL-10); Chinese hamster ovary cells (Urlab et al., “Isolation of Chinese Hamster Cell Mutants Deficient in Dihydrofolate Reductase Activity.” Proc. Natl. Acad. Sci. U.S.A. 77:4216-4220 (1980), which is hereby incorporated by reference in its entirety); mouse sertoli cells (TM4; Mather J. P., “Establishment and Characterization of Two Distinct Mouse Testicular Epithelial Cell Lines,” Biol. Reprod. 23:243-252 (1980), which is hereby incorporated by reference in its entirety); monkey kidney cells (CVI-76, ATCC CCL-70); African green monkey kidney cells (VERO-76, ATCC CRL-1587); human cervical carcinoma cells (HELA, ATCC CCL-2); canine kidney cells (MDCK, ATCC CCL-34); buffalo rat liver cells (BRL 3A, ATCC CRL-1442); human lung cells (W138, ATCC CCL-75); human liver cells (Hep G2, HB 8065); mouse mammary tumor cells (MMT 060562, ATCC CCL-51); rat hepatoma cells (HTC, MI.54; Baumann ct al., “Dexamethasone Regulates the Program of Secretory Glycoprotein Synthesis in Hepatoma Tissue Culture Cells,”J. Cell Biol. 85:1-8 (1980), which is hereby incorporated by reference in its entirety); and TRI cells (Mather et al., “Culture of Testicular Cells in Hormone-Supplemented Serum-Free Medium,” Annals N.Y. Acad. Sci. 383:44-68 (1982), which is hereby incorporated by reference in its entirety). Expression vectors for these cells ordinarily include (if necessary) DNA sequences for an origin of replication, a promoter located 5′ of the nucleic acid sequence to be expressed, a ribosome binding site, an RNA splice site, a polyadenylation site, and/or a transcription terminator site.

Promoters used in mammalian expression vectors can be of viral origin. Such viral promoters may be derived from polyoma virus, adenovirus 2, and simian virus 40 (SV40). The SV40 virus contains two promoters that are termed the early and late promoters. These promoters are useful because they are both easily obtained from the virus as one nucleic acid fragment that also contains the viral origin of replication (Fiers et al., “Complete Nucleotide Sequence of SV40 DNA,” Nature 273:113-120 (1978), which is hereby incorporated by reference in its entirety). Smaller or larger SV40 DNA fragments may also be used, provided they contain the approximately 250-bp sequence extending from the HindIII site toward the BglI site located in the viral origin of replication. Alternatively, promoters that are naturally associated with the foreign gene (homologous promoters) may be used provided that they are compatible with the host cell line selected for transformation.

An origin of replication may be obtained from an exogenous source, such as SV40 or other virus (e.g., polyoma virus, adenovirus, VSV, BPV) and inserted into the expression vector. Alternatively, the origin of replication may be provided by the host cell chromosomal replication mechanism.

Cell-free translation systems are known in the art, and can be used to synthesize heme-binding proteins using the methods of the present invention (see e.g., Kurland, “Translational Accuracy In vitro,” Cell 28:201-202 (1982) and Pavlov et al., “Rate of Translation of Natural mRNAs in an Optimized In vitro System,”Arch. Biochem. Biophys. 328:9-16 (1996); and Cell-Free Translation Systems, Spirin A. S., ed. (2002); Cell-Free Protein Expression, Swartz J. A., ed. (2003), which are hereby incorporated by reference in their entirety). The most frequently used cell-free translation systems consist of extracts from rabbit reticulocytes, wheat germ and E. coli. All are prepared as crude extracts containing all the macromolecular components (70S or 80S ribosomes, tRNAs, aminoacyl-tRNA synthetases, initiation, elongation and termination factors, etc.) required for translation of exogenous RNA. Each extract is supplemented with amino acids, energy sources (ATP, GTP), energy regenerating systems (creatine phosphate and creatine phosphokinase for eukaryotic systems, and phosphoenol pyruvate and pyruvate kinase for the E. coli lysate), and other co-factors (Mg²⁺, K⁺, etc.) that facilitate the function of the particular translation machinery.

Either DNA or RNA, in plasmid or linear form, can be used as the starting material for cell-free protein synthesis. However, DNA starting material is necessarily transcribed to RNA using a “coupled” or “linked” system. A “linked” system generally involves DNA transcription with a bacteriophage polymerase followed by translation in the rabbit reticulocyte lysate or wheat germ lysate. Unlike eukaryotic systems (such as, rabbit reticulocyte or wheat germ) where transcription and translation occur sequentially, transcription and translation occur simultaneously in E. coli cell free systems. Thus. E. coli translation systems are “coupled” and can be performed in the same tube using either a DNA or RNA template. Methods of using E. coli cell-free systems have been described in detail (see e.g., Kigawa et al., “Cell-Free Production and Stable-Isotope Labeling of Milligram Quantities of Proteins,” FEBS Lett. 442:15-19 (1999); Noren et al., “A General Method for Site-Specific Incorporation of Unnatural Amino Acids into Proteins,” Science 244:182-188 (1989); Hanes et al., “In vitro Selection and Evolution of Functional Proteins by Using Ribosome Display,” Proc. Natl. Acad. Sci. U.S.A. 94:4937-4942 (1997); Wilson et al., “The Use of mRNA Display to Select High-Affinity Protein-Binding Peptides,” Proc. Natl. Acad. Sci. U.S.A. 98:3750-3755 (2001); and Sawasaki et al., “A Cell-Free Protein Synthesis System for High-Throughput Proteomics,” Proc. Natl. Acad. Sci. U.S.A. 99(23):14652-14657 (2002), which are hereby incorporated by reference in their entirety). In the E. coli system, it may be advantageous to place a Shine-Dalgarno ribosome binding site upstream of the initiator codon in a DNA template. In particular examples, an E. coli S30 extract system allows expression from DNA vectors containing natural E. coli promoter sequences (such as lac or tac).

Another aspect of the present invention relates to a system for producing functional heme-binding proteins. This system comprises an expression system and one or more expression constructs encoding a recombinant heme-binding protein and a recombinant ferrochelatase.

Suitable expression systems are described above, including cell expression systems (e.g., bacterial, fungal, archaeal, and mammalian) and cell-free expression systems.

The expression constructs of the system will depend on the type of expression system. Suitable expression constructs are described supra. The expression constructs can be in linear form or contained in a plasmid or viral vector, and can further contain regulatory elements, such as a promoter sequence, a ribosome binding sequence, and a nucleic acid molecule encoding a termination sequence, to optimize protein expression. The system of the present invention can include one expression construct encoding both the recombinant heme-binding protein and ferrochelatase. Alternatively, the system can include a first expression construct encoding the recombinant heme-binding protein and a second expression construct encoding the recombinant ferrochelatase.

In accordance with this aspect of the invention, the system for producing functional heme-binding proteins further includes one or more heme precursors. Suitable precursors include δ-amino levulinic acid, succinyl CoA, glycine, glutamate, glutamate-1-semialdehyde, porphobilinogen, hydroxymethylbilane and protoporphyrin as described supra.

Another aspect of the present invention relates to a purified preparation of recombinant functional heme-binding protein.

In a preferred embodiment of the present invention, the purified preparation of functional recombinant heme-binding protein is prepared in accordance with the methods described supra. The purified preparation of recombinant heme-binding protein of the present invention has full heme incorporation and does not contain metal-free porphyrin. The purity of the preparation is assessed by the presence or absence of un-metallated heme, which can be measured by fluorescence spectroscopy and resonance Raman spectroscopy. In a preferred embodiment of the invention, the purified preparation of functional, recombinant heme-binding protein of the present invention is not fluorescent when excited at a wavelength of 397 nm and has a resonance Raman spectrum showing no evidence of free-base porphyrin incorporation (see Examples infra).

Purified preparations of heme-binding proteins of the present invention have a variety of therapeutic, research, and commercial utilities. With regard to therapeutic applications, purified preparations of hemoglobin are desired. Hemoglobin is a heme-binding protein responsible for carrying and delivering oxygen to tissues and organs in animals. Recombinant hemoglobin preparations are used as effective and safe oxygen carriers as an alternative to blood transfusion. A purified preparation of recombinant hemoglobin prepared in accordance with the methods of the present invention is fully functional due to full heme incorporation. Accordingly, the incorporation of a purified preparation of hemoglobin of the present invention into oxygen carrier and blood substitute technologies would improve the oxygen carrying capacity. Suitable oxygen carrier and blood substitute technologies include, without limitation, those disclosed in U.S. Pat. No. 4,412,989 to Iwashits et al., U.S. Pat. No. 6,022,849 to Olsen et al, U.S. Pat. No. 7,329,641 to Fronticellie et al., and U.S. Patent Publication No. 2006/0088583 to Takeoka et al., which are hereby incorporated by reference in their entirety.

Cytochrome P450s are a superfamily of enzymes that are critical to human drug metabolism. These proteins have been implicated in many clinical cases of adverse drug reaction and toxicity stemming from mechanism-based enzyme inhibition and drug-drug interactions. High-throughput assays that identify molecules that inhibit or induce CYP450 early in the drug development process are invaluable for guiding the elimination of candidate drugs that have unwanted metabolic properties and facilitating the production of better clinical candidates. Purified preparations of cytochrome P450 proteins of the present invention will benefit the variety of well established preclinical screening assays that are designed to predict drug metabolism and toxicity (see e.g., Trubetskoy et al., “Highly Minaturized Formats for In Vitro Drug Metabolism Assays Using Vivid Fluorescent Substrate and Recombinant Human Cytochrome P450 Enzymes,” J Bimolecular Screening 56-66 (2005); Donato et al., “Fluorescence-Based Assays for Screening Nine Cytochrome P450 (P450) Activities in Intact Cells Expression Individual Human P450 Enzymes,” Drug Metab. Disposition 32(7):699-706 (2004); Zhang et al., “Cytochrome P450 Reaction-Phenotyping: An Industrial Perspective,” Expert Opin, Drug Metab. Toxicol. 3(5):667-87 (2007); Buters et al., “A Highly Sensitive Tool for the Assay of Cytochrome P450 Enzyme Activity in Rat, Dog, and Man: Direct Fluorescence Monitoring of the Deethylation of 7-ethoxy-4-trifluoromethylcoumarin,” Biochem. Pharm. 46(20):1577-1584 (1993); and Yim et al., “A Continuous Spectrophotometric Assay for NADPH-cytochrome P450 Reductase Activity Using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide,” J. Biochem. Mol. Biol. 38(3):366-369 (2005), which are hereby incorporated by reference in their entirety).

Purified preparations of nitric oxide synthases of the present invention also have therapeutic utility with regard to the treatment of vascular diseases, cancer, microbial infections, tissue injury, and neurological pathologies, and for promoting wound healing (see e.g., U.S. Pat. No. 5,658,565 to Billiar et al., U.S. Patent Publication No. 20070071725 to Paterson et al., and U.S. Patent Publication No. 20100087370 to Jain et al., which are hereby incorporated by reference in their entirety).

Purified preparations of cyclooxygenases (e.g. COX-1, COX-2, COX-3) of the present invention also have therapeutic/commercial utility. These enzymes mediate the formation of prostanoids, including prostaglandins, prostacyclin, and thromboxane. Pharmacological inhibition of these enzymes provides relief from the symptoms of inflammation and pain. Accordingly, purified preparations of cyclooxygenases made in accordance with the present invention can be used to screen and identify, using methods described infra, more potent inhibitors than the currently available non-steroidal anti-inflammatory drugs.

In addition to therapeutic utility, purified preparations of heme-binding proteins also have biotechnological utilities. For example, lignin peroxidase is a heme-binding protein that degrades plant lignin, a heterogenous aromatic polymer that encases the cellulose fibers of lignocellulose (see Weng et al., “Emerging Strategies in Lignin Engineering and Degradation for Cellulosic Biofuel Production,” Curr. Opin. Biotech. 19:166-172 (2008), which is hereby incorporated by reference in its entirety). The lignocellulose content of plant biomass is a primary source of renewable carbon that can be used to produce bio-ethanol and chemical feedstocks for commercial use. A major obstacle to exploiting this renewable carbon source is the presence and degradation of lignin. Therefore, lignin degradation via lignin peroxidase, offers an attractive strategy for optimizing biofuel production (see e.g., U.S Patent Application Publication Nos. 2010/0291653 to Ness et al., 2010/0017916 to Pappan et al., 2005/0233423 to Berka et al., which are hereby incorporated by reference in their entirety). Accordingly, the present invention contemplates the use of a purified preparation of lignin peroxidase that does not contain metal-free porphyrin, for biofuel production.

Another aspect of the present invention relates to a method of identifying an agent that modulates activity of a heme-binding protein. This method involves providing a candidate agent and providing a recombinant functional heme-binding protein. This method further involves contacting the candidate agent with the recombinant functional heme-binding protein under conditions at which the functional heme-binding protein is active and comparing the activity of the functional heme-binding protein as a result of said contacting to the activity of the heme-binding protein alone, both under said conditions at which the heme-binding protein is active. A candidate agent that modulates the activity of a heme-binding protein is identified based on said comparing.

A related aspect of the present invention relates to a method of evaluating the metabolism of an agent by a heme-binding protein. This method involves providing a candidate agent and providing a recombinant functional heme-binding protein. This method further involves contacting the candidate agent with the recombinant functional heme-binding protein under conditions at which the functional heme-binding protein is active and comparing the activity of the functional heme-binding protein as a result of said contacting to the activity of the heme-binding protein alone, both under said conditions at which the heme-binding protein is active. The metabolism of the candidate agent by a heme-binding protein is evaluated based on said comparing.

In one embodiment of this aspect of the present invention, the above methods further involve providing a heme-binding protein substrate and/or one or more heme-binding protein co-factors, where the heme-binding protein substrate and/or heme-binding co-factor is present when the heme-binding protein is contacted with the candidate agent. The activity of the heme-binding protein in the presence of the one or more heme-binding cofactors with the heme-binding protein substrate is evaluated. In other words, the rate of metabolism or conversion of the heme-binding protein substrate by the heme-binding protein is a measure of heme-binding protein activity.

In accordance with this aspect of the present invention a decrease in the activity of the functional heme-binding protein in the presence of the candidate agent compared to in the absence of the candidate agent identifies an agent that modulates the heme-binding protein activity. In this regard, the candidate agent is an inhibitor of heme-binding protein activity. Activators of heme-binding protein activity can also be identified using this assay. An increase in heme-binding protein activity would identify candidate activators of heme-binding protein activity.

In accordance with aspects of the present invention directed to evaluating the metabolism of an agent by a heme-binding protein, an increase in the activity of a functional heme-binding protein in the presence of the candidate agent compared to in the absence of the candidate agent may identify an agent that is a substrate for heme-binding protein metabolism. Alternatively, a decrease in heme-binding protein activity may also indicate that the candidate agent is a substrate for the heme-binding protein activity (e.g., a substrate for cytochrome P450 metabolism). In this case, the observed decrease in heme-binding protein activity would result from competition for heme-protein binding between the heme-binding protein substrate and the candidate agent. In either case, metabolism of the candidate agent by the heme-binding protein can be further evaluated by analyzing the metabolic profile of the candidate agent using methods known in the art (e.g., high-performance liquid chromatography).

In accordance with these aspects of the present invention, the recombinant functional heme-binding protein does not contain metal-free porphyrin. Any of the heme-binding proteins described supra can be utilized in this aspect of the invention. In one embodiment of the present invention, the heme-binding protein is a cytochrome P450 protein. As noted above, the cytochrome P450 family of enzymes are the major catalysts for the oxidative metabolism of a vast array of hydrophobic chemicals. These enzymes are involved in the metabolism or biotransformation of endogenous as well as exogenous hydrophobic compounds. Since cytochrome P450-mediated metabolism influences drug clearance, toxicity, activation, and in some cases, adverse interaction with other drugs, accurately identifying agents that modulate cytochrome P450 activity or serve as substrates for metabolism is particularly important for early toxicological screening of candidate drugs. A number of cytochrome P450 screening assays are known in the art (see e.g., Donato et al., “Fluorescence-Based Assays for Screening Nine Cytochrome P450 (P450) Activities in Intact Cells Expression Individual Human P450 Enzymes,” Drug Metab. Disposition 32(7):699-706 (2004); Zhang et al., “Cytochrome P450 Reaction-Phenotyping: An Industrial Perspective,” Expert Opin. Drug Metab. Toxicol, 3(5):667-87 (2007); Buters et al., “A Highly Sensitive Tool for the Assay of Cytochrome P450 Enzyme Activity in Rat, Dog, and Man: Direct Fluorescence Monitoring of the Deethylation of 7-ethoxy-4-trifluoromethylcoumarin,” Biochem, Pharm. 46(20):1577-1584 (1993); and Yim et al., “A Continuous Spectrophotometric Assay for NADPH-cytochrome P450 Reductase Activity Using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide,” J. Biochem. Mol. Biol. 38(3066-369 (2005), which are hereby incorporated by reference in their entirety) and are commercially available, especially in high-throughput formats (see e.g. Promega. Invitrogen, and Agilent Technologies), all of which can be utilized in these aspects of the present invention, Incorporation of purified preparations of recombinant cytochrome P450 proteins of the present invention will enhance the accuracy of these and other P450 enzyme assays known and used in academic and pharmaceutical research arenas.

EXAMPLES

The following examples illustrate various methods for compositions in the treatment method of the invention. The examples are intended to illustrate, but in no way limit, the scope of the invention.

Materials and Methods for Examples 1-3:

Co-expression of Ferrochelatase with gsNOS. Ferrochelatase (FC) and gsNOS were expressed from the same pACYCduet vector (Novagen). To clone FC, genomic DNA was extracted from E. coli BL21(DE3) cells with the genomic DNA extraction kit from Epicenter. The FC gene was then PCR-amplified (Phusion polymerase, New England Biolabs) from the genomic DNA with primers that generated Nde1 and Xho1 sites at the 5′ and 3′ ends of the gene, respectively. A stop codon was introduced into the 3′ primer before the Xho1 site to prevent C-terminal attachment of the vector-supplied S-tag. The amplified FC gene was then cloned into the Nde1 and Xho1 sites in Multiple Cloning Site-2 of the pACYCdtiet vector. The gene for gsNOS was derived from a previous pET28a-gsNOS plasmid (Sudhamsu et al., “Structure and Reactivity of a Thermostable Prokaryotic Nitric-Oxide Synthase that Forms a Long-Lived Oxy-Heme Complex,” J. Biol. Chem. 281:9623-9632 (2006), which is hereby incorporated by reference in its entirety) by digesting the vector with Nco1 and Xho1 so as to include the His-tag and the thrombin cleavage site along with the coding sequence for gsNOS in the excised fragment. The Nco1-Xho1 fragment was then cloned into the pACYCduet-FC plasmid between the Nco1 and Sal1 sites. Sal1 and Xho1 produce compatible cohesive ends and thereby allow the His-tag, thrombin cleavage site and gsNOS fragment to be cloned between the Nco1 and Sal1 sites of Multiple cloning site-1 of the pACYCduct-FC plasmid. The resulting pACYCduet plasmid allows over-expression of gsNOS with a cleavable His-tag and FC with no tag, GsNOS was expressed and purified as reported before (Sudhamsu et al., “Structure and Reactivity of a Thermostable Prokaryotic Nitric-Oxide Synthase that Forms a Long-Lived Oxy-Heme Complex,” J. Biol. Chem. 281:9623-9632 (2006), which is hereby incorporated by reference in its entirety). Co-expression of gsNOS and FC was also performed similarly to expression of gsNOS alone, although, a lesser amount of δ-ALA was added at the time of induction (10 mg/L versus 25 mg/L for gsNOS), and the growth media was supplemented with 100 μM FeCl₃. The antibiotics, chloramphenicol (34 μg/L) and kanamycin (50 μg/L), were added to the growth media of pACYCduet-gsNOS-FC and pET28a-gsNOS plasmids, respectively.

Co-expression of Ferrochelatase with BP450 and HBPAS. For co-expression of BP450 and HBPAS the same procedure was used. BP450 (NCBI: CBG70284) was cloned into pET151/D-TOPO (Invitrogen), a directional cloning vector with an N-terminal 6×His-Tag followed by a TEV cleavage site and an ampicillin selectable marker. HBPAS (NCBI: NP_(—)248866) was cloned into pET28a (Novagen) using NdeI and HindIII restriction sites, which included an N-terminal 6×His-Tag followed by a thrombin cleavage site and a kanamycin selectable maker. Competent E. coli BL21 (DE3) cells containing FC/pACYCduet were transformed with either BP450/pET151/D-TOPO or HBPAS/pET28. Cells were grown at 37° C. in Luria broth containing 20 ug/mL Cm and 100 ug/L Amp (BP450) or 50 ug/L Kan (HBPAS) to an OD=0.6-0.8, Prior to induction with IPTG, the temperature was reduced to 17° C. and 25 mg/L d-ALA was added to the growth media. Cells were harvested 18-20 hrs after induction. An identical procedure with cells lacking the FC plasmid was used to express BP450 and HBPAS without FC. Both proteins were purified using Ni-NTA (Qiagen) chromatography techniques following the manufacturer's protocol. Furthermore, the proteins were purified to >95% purity using size exclusion chromatography after the removal of 6×His.

Spectroscopy. Resonance Raman and UV-Visible spectra were recorded as described previously (Kabir et al., “Substrate-Ligand Interactions in Geobacillus Stearothermophilus Nitric Oxide Synthase,” Biochem. 47:12389-12397 (2008), which is hereby incorporated by reference in its entirety).

Materials. Sodium chloride was obtained from Mallinkrodt, Ferric Chloride, IPTG and TRIS were from Fisher Scientific, Kanamycin, and Chloramphenicol from USBiological. δ-ALA was obtained from Sigma-Aldrich.

Example 1 UV-Vis Spectroscopy and SDS-PAGE Analysis of gsNOS

GsNOS (Geobacillus stearothermophihis Nitric Oxide Synthase) is a thermophilic enzyme that forms a highly stable heme-oxygen complex (Sudhamsu et al., “Structure and Reactivity of a Thermostable Prokaryotic Nitric-Oxide Synthase that Forms a Long-Lived Oxy-Heme Complex,”J. Biol. Chem. 281:9623-9632 (2006), which is hereby incorporated by reference in its entirety) that has helped in identification of catalytic intermediates responsible for L-arginine oxidation to nitric oxide (Davydov et al., “EPR And ENDOR Characterization of the Reactive Intermediates in the Generation of NO by Cryoreduced Oxy-Nitric Oxide Synthase from G. Stearotherniophilus,”J. Am. Chem. Soc. 131:14493-14507 (2009), which is hereby incorporated by reference in its entirety). In the over-expression of heme proteins the heme precursor δ-ALA is routinely added to the growth media when protein production is induced. Such δ-ALA supplementation results in complete heme incorporation for two other bacterial NOS proteins: B. subtilis NOS (Pant et al., “Structure of a Nitric Oxide Synthase Heme Protein from Bacillus Subtilis,” Biochem. 41:11071-11079 (2002), which is hereby incorporated by reference in its entirety) and D. radiodurans NOS (Buddha et al., “Regioselective Nitration of Tryptophan by a Complex Between Bacterial Nitric-Oxide Synthase and Tryptophanyl-Trna Synthetase,” J. Biol. Chem. 279:49567-49570 (2004), which is hereby incorporated by reference in its entirety). However, in what follows, it is shown that gsNOS over-expressed and purified from E. coli consists of two species: native heme-containing gsNOS, and gsNOS with protoporphyrin IX (free-base porphyrin) bound instead of heme. It was hypothesized that co-expression of ferrochelatase, the enzyme that metallates porphyrin would ameliorate this problem.

A UV-Vis spectroscopic analysis of gsNOS over-expressed in E. coli shows that the amount of heme incorporated with the protein changes from batch to batch, with the ratio of Soret peak height (403 nm) to protein peak height (280 nm) (Abs₄₀₃/Abs₂₈₀) varying between 0.25-0.40. Co-expression of FC with gsNOS increased Abs₄₀₃/Abs₂₈₀ to 0.6 (FIG. 1) in several (>3) different protein expression trials. Thus, gsNOS co-expressed with FC increases heme content of the protein in a consistent fashion.

Purified gsNOS, when over-expressed in E. coli with 25 mg/L δ-ALA added at the time of induction, results in two bands of −42 kDa on SDS-PAGE (FIG. 1, Inset, lane A). Both bands shift on His-tag cleavage (FIG. 1, Inset, lane B), which indicates that both species represent recombinant gsNOS, with an intact N-terminus, but different gel mobilities. MALDI mass spectrometry of the sample shows only one sharp peak at a mass consistent with that of full-length gsNOS, which rules out proteolysis as the factor distinguishing the proteins represented in the two bands. Thus, the apparent difference in molecular weight on the gel of the two species (˜3 kDa) may rather stem from a net charge difference. GsNOS, when co-expressed with FC from E. coli BL21 (DE3) cells, results in only a single species on SDS-PAGE (which corresponds to the lower band of the two observed previously; FIG. 1 Inset, lane C). On His-tag cleavage, this band shifts as a single species (FIG. 1, Inset, lane D). Protoporphyrin IX is the penultimate product in the heme biosynthesis pathway. FC catalyzes the last step in heme biosynthesis, i.e., insertion of an iron atom into protoporphyrin IX. Thus, the presence of two bands in the absence of FC co-expression suggests that protoporphyrin IX, rather than heme, has been incorporated into a substantial fraction of the sample.

Example 2 Resonance Raman and Fluorescence Analysis of gsNOS

Confirmation of porphyrin incorporation into gsNOS came from resonance Raman studies of gsNOS in the presence of substrate L-arginine. A sample of gsNOS (expressed without FC) in the presence of substrate L-arginine, shows vibrational frequencies at 662 cm⁻¹, 738 cm⁻¹, 783 cm⁻¹, 1360 cm⁻¹ and 1543 cm⁻¹, apart from the typical vibrational frequencies that have been previously observed (Santolini et al., “Resonance Raman Study of Bacillus Subtilis NO Synthase-Like Protein: Similarities and Differences with Mammalian NO Synthases,” Biochem. 45:1480-1489 (2006) and Rousseau et al., “Ligand-Protein Interactions in Nitric Oxide Synthase,” J. Inorganic Biochem. 99:306-323 (2005), which are hereby incorporated by reference in their entirety) for other NOSs (FIG. 2B). These additional bands disappear after exposure to laser (FIG. 2C) and the subsequent difference spectrum (before and after laser exposure) highlights the original, additional resonances (FIG. 2D). The frequencies of these vibrations indicates the presence of free-base porphyrin in the protein sample (Blackwood et al., “Alternative Modes of Substrate Distortion in Enzyme and Antibody Catalyzed Ferrochelation Reactions,” Biochem. 37:779-782 (1998) and Lu et al., “Binding of Protoporphyrin IX and Metal Derivatives to the Active Site of Wild-Type Mouse Ferrochelatase at Low Porphyrin-to-Protein Ratios,” Biochem. 41:8253-8262 (2002), which are hereby incorporated by reference in their entirety). Furthermore, the enhanced photosensitivity of porphyrin compared to heme explains why the bands disappear after exposure (Blackwood et al., “Alternative Modes of Substrate Distortion in Enzyme and Antibody Catalyzed Ferrochelation Reactions,” Biochem. 37:779-782 (1998) and Lu et al., “Binding of Protoporphyrin IX and Metal Derivatives to the Active Site of Wild-Type Mouse Ferrochelatase at Low Porphyrin-to-Protein Ratios,” Biochem. 41:8253-8262 (2002), which are hereby incorporated by reference in their entirety). The presence of free base porphyrin is corroborated by the fluorescence spectrum of gsNOS (FIG. 2E), which when measured with excitation at 397 nm shows definitive characteristics of un-metallated heme (Lozovaya et al., “Protoporphyrin-IX as a Possible Ancient Photosensitizer—Spectral and Photochemical Studies,” Origins Of Life And Evolution Of The Biosphere 20:321-330 (1990), which is hereby incorporate by reference in its entirety). Iron-bound heme is not fluorescent when excited at this wavelength. Incorporation of free-base porphyrin suggests that the last step of heme biosynthesis, i.e., Fe metallation to heme, which is performed by FC, cannot keep pace with protein folding and porphyrin incorporation. Co-expression of FC from E. coli with gsNOS and addition of 10 mg/L of δ-ALA of (60 μM, ˜$0.50 per liter) generates a sample that is non-fluorescent and whose resonance Raman spectrum shows no evidence of porphyrin (FIG. 2A). Thus, under these conditions, the protein is fully heme incorporated.

Whether foregoing the addition of δ-ALA was also tested, while co-expressing FC would result in complete heme incorporation of gsNOS. The purified protein, which was checked for heme content by both SDS-PAGE and by UV-Vis spectroscopy had a higher degree of home incorporation than under conditions of only adding δ-ALA (heme:protein ratio ˜0.5); however, heme incorporation was not complete, Addition of a small amount of δ-ALA, (10 mg/L) is sufficient to make up for the slow rate of δ-ALA biosynthesis and produce fully incorporated protein in the presence of FC.

Example 3 FC-Assisted Heme Incorporation for a Bacterial P450 (BP450) and a Heme-Binding PAS Protein (HBPAS)

In addition to gsNOS, FC also increases heme content to saturating levels in two other unrelated proteins: BP450, a Cys-ligated heme protein and HBPAS: a His-ligated heme protein. Both of these proteins, when over-expressed in E. coli, are produced with partial heme incorporation, UV-Vis spectra of purified BP450 and BP450 co-expressed with FC are strikingly different, with the increased intensity of the Soret peak indicative of greater heme content in the material produced along with FC (FIG. 3), When analyzed by SDS-PAGE, BP450 again also shows two bands (FIG. 3, Inset, lane A), which both shift on His-tag cleavage (FIG. 3, Inset, lane B). Like gsNOS, on co-expression with FC (FIG. 3, Inset, lane C) BP450 produces only one band, which shifts on His-tag cleavage (FIG. 3, Inset, lane D). In contrast, HBPAS always results in a single band on a SDS-PAGE gel, but shows absorption for four Q-bands in the UV-Vis spectrum when produced without FC (FIG. 4, thin line). Pure heme proteins show only two such bands. The extra band(s) are representative of protein bound protoporphyrin IX (Lozovaya et al., “Protoporphyrin-IX as a Possible Ancient Photosensitizer—Spectral and Photochemical Studies,” Origins Of Life And Evolution Of The Biosphere 20:321-330 (1990), which is hereby incorporated by reference in its entirety). Co-expression of FC results in an increase in the Abs_((Soret))/Abs₍₂₈₀₎ and the extra Q-bands disappear (FIG. 4, thick line). The fluorescence spectrum of HBPAS without co-expression of FC is similar to that of gsNOS expressed without FC, but this fluorescence, attributable to protoporphyrin IX, also disappears on co-expression with FC.

Discussion of Examples 1-3

The production of δ-ALA is a rate-limiting step for heme biosynthesis (Ades, I. Z., “Heme Production in Animal-Tissues—The Regulation of Biogenesis of Delta-Aminolevulinate Synthase,” Internat. Biochem. 22:565-578 (1990); Woodard et al., “Regulation of Heine-Biosynthesis in Escherichia-Coli,” Archives Biochem. Biophys. 316:110-115 (1995); Gibson et al., “Is Delta-Aminolevulinic Acid Dehydratase Rate Limiting in Heme Biosynthesis Following Exposure of Cells to Delta-Aminolevulinic Acid?” Photochem. Photobiol. 73:312-317 (2001); and Heinemann et al., “The Biochemistry of Heme Biosynthesis,” Archives Biochem. Biophys. 474:238-251 (2008), which are hereby incorporated by reference in their entirety) and δ-ALA synthesis is itself slowed by heme feedback inhibition. Thus, as has been well recognized (Ades, I. Z., “Heme Production in Animal-Tissues—The Regulation of Biogenesis of Delta-Aminolevulinate Synthase,”Internat. J. Biochem. 22:565-578 (1990); Woodard et al., “Regulation of Heme-Biosynthesis in Escherichia-Coli,” Archives Biochem. Biophys. 316:110-115 (1995); Gibson et al., “Is Delta-Aminolevulinic Acid Dehydratase Rate Limiting in Heme Biosynthesis Following Exposure of Cells to Delta-Aminolevulinic Acid?” Photochem. Photobtol. 73:312-317 (2001); and Heinemann et al., “The Biochemistry of Heme Biosynthesis,”Archives Biochem. Biophys. 474:238-251 (2008), which are hereby incorporated by reference in their entirety), feeding with δ-ALA greatly aids recombinant heme protein production in E. coli. However, as demonstrated herein, under conditions of augmentation with δ-ALA, ferrous iron insertion into protoporphyrin IX becomes rate-limiting. Co-expression with ferrochelatase along with the addition of a small amount of δ-ALA, is sufficient to produce fully incorporated heme protein. This method is applicable for both Cys-ligated and His-ligated heme proteins. In the case of the two Cys-ligated proteins, porphyrin substitution could be observed on an SDS-PAGE gel as two closely spaced bands and also by fluorescence spectroscopy. In the one His-ligated heme protein example, UV-Visible and fluorescence spectra were effective indicators of insufficient porphyrin metallation, but only one band was observed by SDS-PAGE even with less than full porphyrin content. This is probably because the heme or porphyrin does not remain associated with the PAS protein during electrophoresis, unlike the other two cases.

In conclusion, the simple and inexpensive method of co-expressing ferrochelatase is effective at producing fully incorporated heme proteins in E. coli.

Example 4 FC-Assisted Heme Incorporation for Full-Length NOS

Co-expression of FC and full-length NOS was carried out as described supra. Under normal conditions of expression the Soret band indicative of heme incorporation is barely visible above the flavin absorption of the reductase component (gray trace), however, upon co-expression with ferrochelatase a strong Soret absorption peak (at ˜416 nm), indicative of heme incorporation becomes apparent (black trace). Total yields of full-length NOS also increase in the presence of ferrochelatase as indicated by the overall greater cofactor absorption profile.

Although preferred embodiments have been depicted and described in detail herein, it will be apparent to those skilled in the relevant art that various modifications, additions, substitutions, and the like can be made without departing from the spirit of the invention and these are therefore considered to be within the scope of the invention as defined in the claims which follow. 

What is claimed is:
 1. A method of producing a functional recombinant heme-binding protein comprising: providing an Escherichia coli host cell that expresses endogenous ferrochelatase protein; co-expressing in said host cell a non-native recombinant heme-binding protein and a recombinant ferrochelatase protein or a polypeptide thereof which retains iron insertion activity, wherein said host cell expresses both the endogenous ferrochelatase protein and the recombinant ferrochelatase protein or polypeptide thereof; and culturing said host cell under conditions effective for complete heme incorporation into the recombinantly produced heme-binding protein, thereby producing a functional heme-binding protein that does not contain metal-free porphyrin.
 2. The method of claim 1, wherein said co-expressing is carried out in the presence of one or more heme precursors.
 3. The method of claim 2, wherein the heme precursor is selected from the group consisting of δ-amino levulinic acid, succinyl CoA, glycine, glutamate, glutamate-1-semialdehyde, porphobilinogen, hydroxymethylbilane, and protoporphyrin.
 4. The method of claim 1 further comprising: providing one or more expression vectors encoding the recombinant heme-binding protein and the recombinant ferrochelatase.
 5. The method of claim 4, wherein the one or more expression vectors comprise one expression vector encoding both the recombinant heme-binding protein and the recombinant ferrochelatase.
 6. The method of claim 4, wherein the one or more expression vectors comprise a first expression vector encoding the recombinant heme-binding protein and a second expression vector encoding ferrochelatase.
 7. The method of claim 4, wherein the one or more expression vectors is selected from the group consisting of a bacterial expression vector, fungal expression vector, baculoviral expression vector, plant expression vector, archaeal expression vector, and mammalian expression vector.
 8. The method of claim 1, wherein the heme-binding protein is a mammalian heme-binding protein.
 9. The method of claim 1, wherein the heme-binding protein is a bacterial heme-binding protein.
 10. The method of claim 1, wherein the heme-binding protein is a member of a class of heme-binding proteins selected from the group consisting of globins, cytochromes, bacterioferritins, hydroxylamine oxidoreductases, nitrophorins, peroxidases, cyclooxygenases, catalases, cytochromes P-450s, chloroperoxidases, PAS-domain heme sensors, H-NOX heme sensors, and nitric oxide synthases.
 11. A system for producing functional heme-binding proteins comprising: one or more expression constructs encoding a non-native recombinant heme-binding protein and a recombinant ferrochelatase and an Escherichia coli host cell that expresses endogenous ferrochelatase protein and contains said one or more expression constructs.
 12. The system of claim 11 further comprising: one or more heme precursors.
 13. The system of claim 12, wherein the one or more heme precursors is selected from the group consisting of δ-amino levulinic acid, succinyl CoA, glycine, glutamate, glutamate-1-semialdehyde, porphobilinogen, hydroxymethylbilane, and protoporphyrin.
 14. The system of claim 11, wherein the one or more expression constructs further comprise a promoter sequence, a nucleic acid encoding a ribosome binding sequence, and a nucleic acid encoding a termination sequence.
 15. The system of claim 11, wherein the one or more expression constructs is selected from the group consisting of a linear DNA construct, a plasmid vector, and a viral vector.
 16. The system of claim 11, wherein the one or more expression constructs comprise one expression construct encoding both the recombinant heme-binding protein and the recombinant ferrochelatase.
 17. The system of claim 11, wherein the one or more expression constructs comprise a first expression construct encoding the recombinant heme-binding protein and a second expression construct encoding the recombinant ferrochelatase.
 18. The system of claim 11, wherein the one or more expression constructs is selected from the group consisting of a bacterial expression vector, fungal expression vector, baculoviral expression vector, plant expression vector, archaeal expression vector, and mammalian expression vector.
 19. The system of claim 11, wherein the heme-binding protein is a mammalian heme-binding protein.
 20. The system of claim 11, wherein the heme-binding protein is a bacterial heme-binding protein.
 21. The system of claim 11, wherein the heme-binding protein is a member of a class of heme-binding proteins selected from the group consisting of cytochromes, bacterioferritins, hydroxylamine oxidoreductases, nitrophorins, peroxidases, cyclooxygenases, catalases, cytochromes P-450s, chloroperoxidases, globins, PAS-domain heme sensors, H-NOX heme sensors, and nitric oxide synthases.
 22. The system of claim 11, wherein the system produces a functional heme-binding protein that does not contain metal-free porphyrin.
 23. A method of producing a functional recombinant heme-binding protein comprising: providing a bacterial or yeast host cell that expresses endogenous ferrochelatase protein; co-expressing in said host cell (i) a non-native recombinant heme-binding protein that is a member of a class of heme-binding proteins selected from the group consisting of cytochrome P450s, PAS-domain heme sensors, and nitric oxide synthases, and (ii) a recombinant ferrochelatase protein or a polypeptide thereof which retains iron insertion activity, wherein said host cell expresses both the endogenous ferrochelatase protein and the recombinant ferrochelatase protein or polypeptide thereof; and culturing said host cell under conditions effective for complete heme incorporation into the recombinantly produced heme-binding protein, thereby producing a functional heme-binding protein that does not contain metal-free porphyrin.
 24. The method of claim 23, wherein said co-expressing is carried out in the presence of one or more heme precursors.
 25. The method of claim 24, wherein the heme precursor is selected from the group consisting of δ-amino levulinic acid, succinyl CoA, glycine, glutamate, glutamate-1-semialdehyde, porphobilinogen, hydroxymethylbilane, and protoporphyrin.
 26. The method of claim 23 further comprising: providing one or more expression vectors encoding the recombinant heme-binding protein and the recombinant ferrochelatase.
 27. The method of claim 26, wherein the one or more expression vectors comprise one expression vector encoding both the recombinant heme-binding protein and the recombinant ferrochelatase.
 28. The method of claim 26, wherein the one or more expression vectors comprise a first expression vector encoding the recombinant heme-binding protein and a second expression vector encoding ferrochelatase.
 29. The method of claim 26, wherein the one or more expression vectors is selected from the group consisting of a bacterial expression vector, fungal expression vector, baculoviral expression vector, plant expression vector, archaeal expression vector, and mammalian expression vector.
 30. The method of claim 23, wherein the heme-binding protein is a mammalian heme-binding protein.
 31. The method of claim 23, wherein the heme-binding protein is a bacterial heme-binding protein.
 32. A system for producing functional heme-binding proteins comprising: one or more expression constructs encoding (i) a non-native recombinant heme-binding protein that is a member of a class of heme-binding proteins selected from the group consisting of cytochrome P450s, PAS-domain heme sensors, and nitric oxide synthases, and (ii) a recombinant ferrochelatase and a bacterial or yeast cell that expresses endogenous ferrochelatase protein and contains said one or more expression constructs.
 33. The system of claim 32 further comprising: one or more heme precursors.
 34. The system of claim 32, wherein the one or more heme precursors is selected from the group consisting of δ-amino levulinic acid, succinyl CoA, glycine, glutamate, glutamate-1-semialdehyde, porphobilinogen, hydroxymethylbilane, and protoporphyrin.
 35. The system of claim 32, wherein the one or more expression constructs further comprise a promoter sequence, a nucleic acid encoding a ribosome binding sequence, and a nucleic acid encoding a termination sequence.
 36. The system of claim 32, wherein the one or more expression constructs is selected from the group consisting of a linear DNA construct, a plasmid vector, and a viral vector.
 37. The system of claim 32, wherein the one or more expression constructs comprise one expression construct encoding both the recombinant heme-binding protein and the recombinant ferrochelatase.
 38. The system of claim 32, wherein the one or more expression constructs comprise a first expression construct encoding the recombinant heme-binding protein and a second expression construct encoding the recombinant ferrochelatase.
 39. The system of claim 32, wherein the one or more expression constructs is selected from the group consisting of a bacterial expression vector, fungal expression vector, baculoviral expression vector, plant expression vector, archaeal expression vector, and mammalian expression vector.
 40. The system of claim 32, wherein the heme-binding protein is a mammalian heme-binding protein.
 41. The system of claim 32, wherein the heme-binding protein is a bacterial heme-binding protein.
 42. The system of claim 32, wherein the system produces a functional heme-binding protein that does not contain metal-free porphyrin. 